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Additional data for the mouse TRPV6 expression atlas
To identify TRPV6 expression in the whole mouse with a cellular resolution we took advantage of TRPV6-IRES-Cre knock-in mice crossed with the enhanced ROSA26-τGFP reporter line. In the resulting TRPV6-IC/eR26-τGFP animals, TRPV6-expressing cells are labeled with τGFP. Data were collected from organs...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065733/ https://www.ncbi.nlm.nih.gov/pubmed/35516004 http://dx.doi.org/10.1016/j.dib.2022.108201 |
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author | Wartenberg, Philipp Lux, Femke Busch, Kai Fecher-Trost, Claudia Flockerzi, Veit Krasteva-Christ, Gabriela Boehm, Ulrich Weissgerber, Petra |
author_facet | Wartenberg, Philipp Lux, Femke Busch, Kai Fecher-Trost, Claudia Flockerzi, Veit Krasteva-Christ, Gabriela Boehm, Ulrich Weissgerber, Petra |
author_sort | Wartenberg, Philipp |
collection | PubMed |
description | To identify TRPV6 expression in the whole mouse with a cellular resolution we took advantage of TRPV6-IRES-Cre knock-in mice crossed with the enhanced ROSA26-τGFP reporter line. In the resulting TRPV6-IC/eR26-τGFP animals, TRPV6-expressing cells are labeled with τGFP. Data were collected from organs prepared from fixed experimental adult and juvenile TRPV6-IC/eR26τGFP and Cre-negative eR26-τGFP control animals of both sexes. Organ cryosections from each age and sex were stained for GFP and imaged with a slide scanner. Here, we describe reporter gene expression in a large number of tissues. We also document the absence of τGFP signal in the corresponding Cre-negative control tissues, including controls for the TRPV6 expression data described in [1]. The data reported here and in [1] constitute the TRPV6 expression atlas for the mouse. Our data offer a wealth of information to enable investigation of the functional role of TRPV6 channels in different tissues. |
format | Online Article Text |
id | pubmed-9065733 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-90657332022-05-04 Additional data for the mouse TRPV6 expression atlas Wartenberg, Philipp Lux, Femke Busch, Kai Fecher-Trost, Claudia Flockerzi, Veit Krasteva-Christ, Gabriela Boehm, Ulrich Weissgerber, Petra Data Brief Data Article To identify TRPV6 expression in the whole mouse with a cellular resolution we took advantage of TRPV6-IRES-Cre knock-in mice crossed with the enhanced ROSA26-τGFP reporter line. In the resulting TRPV6-IC/eR26-τGFP animals, TRPV6-expressing cells are labeled with τGFP. Data were collected from organs prepared from fixed experimental adult and juvenile TRPV6-IC/eR26τGFP and Cre-negative eR26-τGFP control animals of both sexes. Organ cryosections from each age and sex were stained for GFP and imaged with a slide scanner. Here, we describe reporter gene expression in a large number of tissues. We also document the absence of τGFP signal in the corresponding Cre-negative control tissues, including controls for the TRPV6 expression data described in [1]. The data reported here and in [1] constitute the TRPV6 expression atlas for the mouse. Our data offer a wealth of information to enable investigation of the functional role of TRPV6 channels in different tissues. Elsevier 2022-04-22 /pmc/articles/PMC9065733/ /pubmed/35516004 http://dx.doi.org/10.1016/j.dib.2022.108201 Text en © 2022 The Author(s). Published by Elsevier Inc. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Wartenberg, Philipp Lux, Femke Busch, Kai Fecher-Trost, Claudia Flockerzi, Veit Krasteva-Christ, Gabriela Boehm, Ulrich Weissgerber, Petra Additional data for the mouse TRPV6 expression atlas |
title | Additional data for the mouse TRPV6 expression atlas |
title_full | Additional data for the mouse TRPV6 expression atlas |
title_fullStr | Additional data for the mouse TRPV6 expression atlas |
title_full_unstemmed | Additional data for the mouse TRPV6 expression atlas |
title_short | Additional data for the mouse TRPV6 expression atlas |
title_sort | additional data for the mouse trpv6 expression atlas |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065733/ https://www.ncbi.nlm.nih.gov/pubmed/35516004 http://dx.doi.org/10.1016/j.dib.2022.108201 |
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