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Exosome-derived small non-coding RNAs reveal immune response upon grafting transplantation in Pinctada fucata (Mollusca)
Exosomes, a subset of small extracellular vesicles, carry various nucleic acids, proteins, lipids, amino acids and metabolites. They function as a mode of intercellular communication and molecular transfer. Exosome cargo molecules, including small non-coding RNAs (sncRNAs), are involved in the immun...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065966/ https://www.ncbi.nlm.nih.gov/pubmed/35506205 http://dx.doi.org/10.1098/rsob.210317 |
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author | Huang, Songqian Nishiumi, Shinya Asaduzzaman, Md Pan, Yida Liu, Guanting Yoshitake, Kazutoshi Maeyama, Kaoru Kinoshita, Shigeharu Nagai, Kiyohito Watabe, Shugo Yoshida, Tetsuhiko Asakawa, Shuichi |
author_facet | Huang, Songqian Nishiumi, Shinya Asaduzzaman, Md Pan, Yida Liu, Guanting Yoshitake, Kazutoshi Maeyama, Kaoru Kinoshita, Shigeharu Nagai, Kiyohito Watabe, Shugo Yoshida, Tetsuhiko Asakawa, Shuichi |
author_sort | Huang, Songqian |
collection | PubMed |
description | Exosomes, a subset of small extracellular vesicles, carry various nucleic acids, proteins, lipids, amino acids and metabolites. They function as a mode of intercellular communication and molecular transfer. Exosome cargo molecules, including small non-coding RNAs (sncRNAs), are involved in the immune response in various organisms. However, the role of exosome-derived sncRNAs in immune responses in molluscs remains unclear. Here, we aimed to reveal the sncRNAs involved in the immune response during grafting transplantation by the pearl oyster Pinctada fucata. Exosomes were successfully extracted from the P. fucata haemolymph during graft transplantation. Abundant microRNAs (miRNAs) and PIWI-interacting RNAs (piRNAs) were simultaneously discovered in P. fucata exosomes by small RNA sequencing. The expression patterns of the miRNAs and piRNAs at the grafting and initial stages were not substantially different, but varied significantly between the initial and later stages. Target prediction and functional analysis indicate that these miRNAs and piRNAs are related to immune response upon grafting transplantation, whereas piRNAs may also be associated with transposon silencing by targeting with genome transposon elements. This work provides the basis for a functional understanding of exosome-derived sncRNAs and helps to gain further insight into the PIWI/piRNA pathway function outside of germline cells in molluscs. |
format | Online Article Text |
id | pubmed-9065966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-90659662022-05-09 Exosome-derived small non-coding RNAs reveal immune response upon grafting transplantation in Pinctada fucata (Mollusca) Huang, Songqian Nishiumi, Shinya Asaduzzaman, Md Pan, Yida Liu, Guanting Yoshitake, Kazutoshi Maeyama, Kaoru Kinoshita, Shigeharu Nagai, Kiyohito Watabe, Shugo Yoshida, Tetsuhiko Asakawa, Shuichi Open Biol Research Exosomes, a subset of small extracellular vesicles, carry various nucleic acids, proteins, lipids, amino acids and metabolites. They function as a mode of intercellular communication and molecular transfer. Exosome cargo molecules, including small non-coding RNAs (sncRNAs), are involved in the immune response in various organisms. However, the role of exosome-derived sncRNAs in immune responses in molluscs remains unclear. Here, we aimed to reveal the sncRNAs involved in the immune response during grafting transplantation by the pearl oyster Pinctada fucata. Exosomes were successfully extracted from the P. fucata haemolymph during graft transplantation. Abundant microRNAs (miRNAs) and PIWI-interacting RNAs (piRNAs) were simultaneously discovered in P. fucata exosomes by small RNA sequencing. The expression patterns of the miRNAs and piRNAs at the grafting and initial stages were not substantially different, but varied significantly between the initial and later stages. Target prediction and functional analysis indicate that these miRNAs and piRNAs are related to immune response upon grafting transplantation, whereas piRNAs may also be associated with transposon silencing by targeting with genome transposon elements. This work provides the basis for a functional understanding of exosome-derived sncRNAs and helps to gain further insight into the PIWI/piRNA pathway function outside of germline cells in molluscs. The Royal Society 2022-05-04 /pmc/articles/PMC9065966/ /pubmed/35506205 http://dx.doi.org/10.1098/rsob.210317 Text en © 2022 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Research Huang, Songqian Nishiumi, Shinya Asaduzzaman, Md Pan, Yida Liu, Guanting Yoshitake, Kazutoshi Maeyama, Kaoru Kinoshita, Shigeharu Nagai, Kiyohito Watabe, Shugo Yoshida, Tetsuhiko Asakawa, Shuichi Exosome-derived small non-coding RNAs reveal immune response upon grafting transplantation in Pinctada fucata (Mollusca) |
title | Exosome-derived small non-coding RNAs reveal immune response upon grafting transplantation in Pinctada fucata (Mollusca) |
title_full | Exosome-derived small non-coding RNAs reveal immune response upon grafting transplantation in Pinctada fucata (Mollusca) |
title_fullStr | Exosome-derived small non-coding RNAs reveal immune response upon grafting transplantation in Pinctada fucata (Mollusca) |
title_full_unstemmed | Exosome-derived small non-coding RNAs reveal immune response upon grafting transplantation in Pinctada fucata (Mollusca) |
title_short | Exosome-derived small non-coding RNAs reveal immune response upon grafting transplantation in Pinctada fucata (Mollusca) |
title_sort | exosome-derived small non-coding rnas reveal immune response upon grafting transplantation in pinctada fucata (mollusca) |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9065966/ https://www.ncbi.nlm.nih.gov/pubmed/35506205 http://dx.doi.org/10.1098/rsob.210317 |
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