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Preparation of DNA functional phosphorescent quantum dots and application in melamine detection in milk
Bio-functionalization of quantum dots (QDs) is of important value in practical applications. With single-stranded DNA (ssDNA) rich in thymine T and thioguanine G taken as the template, a new-type nanocomposite material (ssDNA-PQDs) synthesized from low-toxicity T-ssDNA functionalized Mn–ZnS and room...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066003/ https://www.ncbi.nlm.nih.gov/pubmed/35521351 http://dx.doi.org/10.1039/c9ra03919h |
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author | Miao, Yanming Wang, Ruirui Sun, Xiaojie Yan, Guiqin |
author_facet | Miao, Yanming Wang, Ruirui Sun, Xiaojie Yan, Guiqin |
author_sort | Miao, Yanming |
collection | PubMed |
description | Bio-functionalization of quantum dots (QDs) is of important value in practical applications. With single-stranded DNA (ssDNA) rich in thymine T and thioguanine G taken as the template, a new-type nanocomposite material (ssDNA-PQDs) synthesized from low-toxicity T-ssDNA functionalized Mn–ZnS and room-temperature phosphorescent (RTP) QDs (PQDs) was prepared in this paper by optimizing synthesis conditions, and these ssDNA-PQDs could emit orange RTP signals at 590 nm. As these ssDNA-PQDs are rich in T sequences and T sequences can bond with melamine through the hydrogen-bond interaction, ssDNA-PQDs experience aggregation, thus causing phosphorescent exciton energy transfer (PEET) between ssDNA-PQDs of different particle sizes and their RTP quenching. Based on this principle, an RTP detection method for melamine was established. The linear range and detection limit of the detection method are 0.005–6 mM and 0.0016 mM respectively. As this method is based on the RTP nature of ssDNA-PQDs, it can avoid disturbance from background fluorescence and scattered light of the biological fluid, and it is very suitable for melamine detection in the biological fluid milk. |
format | Online Article Text |
id | pubmed-9066003 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90660032022-05-04 Preparation of DNA functional phosphorescent quantum dots and application in melamine detection in milk Miao, Yanming Wang, Ruirui Sun, Xiaojie Yan, Guiqin RSC Adv Chemistry Bio-functionalization of quantum dots (QDs) is of important value in practical applications. With single-stranded DNA (ssDNA) rich in thymine T and thioguanine G taken as the template, a new-type nanocomposite material (ssDNA-PQDs) synthesized from low-toxicity T-ssDNA functionalized Mn–ZnS and room-temperature phosphorescent (RTP) QDs (PQDs) was prepared in this paper by optimizing synthesis conditions, and these ssDNA-PQDs could emit orange RTP signals at 590 nm. As these ssDNA-PQDs are rich in T sequences and T sequences can bond with melamine through the hydrogen-bond interaction, ssDNA-PQDs experience aggregation, thus causing phosphorescent exciton energy transfer (PEET) between ssDNA-PQDs of different particle sizes and their RTP quenching. Based on this principle, an RTP detection method for melamine was established. The linear range and detection limit of the detection method are 0.005–6 mM and 0.0016 mM respectively. As this method is based on the RTP nature of ssDNA-PQDs, it can avoid disturbance from background fluorescence and scattered light of the biological fluid, and it is very suitable for melamine detection in the biological fluid milk. The Royal Society of Chemistry 2019-07-05 /pmc/articles/PMC9066003/ /pubmed/35521351 http://dx.doi.org/10.1039/c9ra03919h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Miao, Yanming Wang, Ruirui Sun, Xiaojie Yan, Guiqin Preparation of DNA functional phosphorescent quantum dots and application in melamine detection in milk |
title | Preparation of DNA functional phosphorescent quantum dots and application in melamine detection in milk |
title_full | Preparation of DNA functional phosphorescent quantum dots and application in melamine detection in milk |
title_fullStr | Preparation of DNA functional phosphorescent quantum dots and application in melamine detection in milk |
title_full_unstemmed | Preparation of DNA functional phosphorescent quantum dots and application in melamine detection in milk |
title_short | Preparation of DNA functional phosphorescent quantum dots and application in melamine detection in milk |
title_sort | preparation of dna functional phosphorescent quantum dots and application in melamine detection in milk |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066003/ https://www.ncbi.nlm.nih.gov/pubmed/35521351 http://dx.doi.org/10.1039/c9ra03919h |
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