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Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction

Photobiomodulation (PBM) has emerged as an alternative therapy involved in modulating a variety of biological effects. In this study, we verified whether PBM can affect cardiac physiological activity in mice through noninvasive irradiation using light-emitting diodes at a wavelength of 630 nm (LED-R...

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Autores principales: Gao, Xinlu, Li, Hanjing, Zhang, Wenwen, Wang, Xiuxiu, Sun, Hongyue, Cao, Yang, Zhao, Yiming, Ji, Haoyu, Yang, Fan, Ma, Wenya, Liu, Yu, Yang, Baofeng, Cai, Benzhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066112/
https://www.ncbi.nlm.nih.gov/pubmed/35541894
http://dx.doi.org/10.7150/ijbs.71440
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author Gao, Xinlu
Li, Hanjing
Zhang, Wenwen
Wang, Xiuxiu
Sun, Hongyue
Cao, Yang
Zhao, Yiming
Ji, Haoyu
Yang, Fan
Ma, Wenya
Liu, Yu
Yang, Baofeng
Cai, Benzhi
author_facet Gao, Xinlu
Li, Hanjing
Zhang, Wenwen
Wang, Xiuxiu
Sun, Hongyue
Cao, Yang
Zhao, Yiming
Ji, Haoyu
Yang, Fan
Ma, Wenya
Liu, Yu
Yang, Baofeng
Cai, Benzhi
author_sort Gao, Xinlu
collection PubMed
description Photobiomodulation (PBM) has emerged as an alternative therapy involved in modulating a variety of biological effects. In this study, we verified whether PBM can affect cardiac physiological activity in mice through noninvasive irradiation using light-emitting diodes at a wavelength of 630 nm (LED-Red). We found that the PBM involved in regulating the repair of injured myocardium is wavelength-limited. LED-Red caused cardiomyocytes (CMs) that had exited the cell cycle to divide and proliferate again, and the cell proliferation ratio increased significantly with the accumulation of intracellular photopower. In addition, LED-Red promoted myocardial revascularization and myocardial regeneration, reduced the area of fibrosis in mice with myocardial infarction (MI), and thus improved cardiac contractile function. In regard to the mechanism, miRNA sequencing analysis showed that low-power LED-Red irradiation could induce differential changes in miRNAs in CMs. Among them, miR-136-5p was identified as a cardiac photo-sensitive miRNA and was obviously inhibited after stimulation, which produced a proliferation-promoting effect on CMs. Subsequent luciferase reporter assays confirmed the involvement of Ino80 as a binding target of miR-136-5p in the regulatory process of CM proliferation. Similarly, LED-Red irradiation elevated intracellular Ino80 expression. After knockdown of Ino80, the proliferation-promoting effect of LED-Red on CMs was inhibited. Collectively, this study demonstrates that LED-Red can promote CM proliferation by inhibiting cardiac photo-sensitive miRNA- miR-136-5p expression through targeting Ino80. The findings provided a new potential strategy for the treatment of ischemic cardiomyopathy (ICD).
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spelling pubmed-90661122022-05-09 Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction Gao, Xinlu Li, Hanjing Zhang, Wenwen Wang, Xiuxiu Sun, Hongyue Cao, Yang Zhao, Yiming Ji, Haoyu Yang, Fan Ma, Wenya Liu, Yu Yang, Baofeng Cai, Benzhi Int J Biol Sci Research Paper Photobiomodulation (PBM) has emerged as an alternative therapy involved in modulating a variety of biological effects. In this study, we verified whether PBM can affect cardiac physiological activity in mice through noninvasive irradiation using light-emitting diodes at a wavelength of 630 nm (LED-Red). We found that the PBM involved in regulating the repair of injured myocardium is wavelength-limited. LED-Red caused cardiomyocytes (CMs) that had exited the cell cycle to divide and proliferate again, and the cell proliferation ratio increased significantly with the accumulation of intracellular photopower. In addition, LED-Red promoted myocardial revascularization and myocardial regeneration, reduced the area of fibrosis in mice with myocardial infarction (MI), and thus improved cardiac contractile function. In regard to the mechanism, miRNA sequencing analysis showed that low-power LED-Red irradiation could induce differential changes in miRNAs in CMs. Among them, miR-136-5p was identified as a cardiac photo-sensitive miRNA and was obviously inhibited after stimulation, which produced a proliferation-promoting effect on CMs. Subsequent luciferase reporter assays confirmed the involvement of Ino80 as a binding target of miR-136-5p in the regulatory process of CM proliferation. Similarly, LED-Red irradiation elevated intracellular Ino80 expression. After knockdown of Ino80, the proliferation-promoting effect of LED-Red on CMs was inhibited. Collectively, this study demonstrates that LED-Red can promote CM proliferation by inhibiting cardiac photo-sensitive miRNA- miR-136-5p expression through targeting Ino80. The findings provided a new potential strategy for the treatment of ischemic cardiomyopathy (ICD). Ivyspring International Publisher 2022-04-18 /pmc/articles/PMC9066112/ /pubmed/35541894 http://dx.doi.org/10.7150/ijbs.71440 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Gao, Xinlu
Li, Hanjing
Zhang, Wenwen
Wang, Xiuxiu
Sun, Hongyue
Cao, Yang
Zhao, Yiming
Ji, Haoyu
Yang, Fan
Ma, Wenya
Liu, Yu
Yang, Baofeng
Cai, Benzhi
Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction
title Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction
title_full Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction
title_fullStr Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction
title_full_unstemmed Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction
title_short Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction
title_sort photobiomodulation drives mir-136-5p expression to promote injury repair after myocardial infarction
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066112/
https://www.ncbi.nlm.nih.gov/pubmed/35541894
http://dx.doi.org/10.7150/ijbs.71440
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