Enzymatic characterization and molecular mechanism of a novel aspartokinase mutant M372I/T379W from Corynebacterium pekinense

A novel aspartokinase mutant M372I/T379W from Corynebacterium pekinense was constructed by using site-directed mutagenesis. The enzyme was then purified, characterized, and its molecular mechanism was comprehensively analyzed. Compared with wild-type AK, the catalytic activity of M372I/T379W AK was...

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Detalles Bibliográficos
Autores principales: Gao, Yunna, Han, Caijing, Liu, Chunlei, Wang, Ji, Zhao, Lan, Fang, Li, Min, Weihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066179/
https://www.ncbi.nlm.nih.gov/pubmed/35521304
http://dx.doi.org/10.1039/c9ra03293b

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066179/
https://www.ncbi.nlm.nih.gov/pubmed/35521304
http://dx.doi.org/10.1039/c9ra03293b

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