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Long-read sequencing infers a mechanism for copy number variation of template for alternative lengthening of telomeres in a wild C. elegans strain

Template for alternative lengthening of telomeres 1 (TALT1) is a specific sequence used to protect chromosomal ends from telomere damage first identified in the CB4856 strain of Caenorhabditis elegans . Here we assembled the genome of DL226, a wild strain with one more copy of TALT1-like sequences i...

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Detalles Bibliográficos
Autores principales: Lee, Bo Yun, Kim, Jun, Lee, Junho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066243/
https://www.ncbi.nlm.nih.gov/pubmed/35622501
http://dx.doi.org/10.17912/micropub.biology.000563
Descripción
Sumario:Template for alternative lengthening of telomeres 1 (TALT1) is a specific sequence used to protect chromosomal ends from telomere damage first identified in the CB4856 strain of Caenorhabditis elegans . Here we assembled the genome of DL226, a wild strain with one more copy of TALT1-like sequences in its genome compared to those of CB4856, using long-read DNA sequencing. We found that a five-copy array of short telomeric repeats and TALT1s present in CB4856 were changed to a six-copy array due to the duplication of the third copy; there was an additional damage-repair trace in the new short telomeric repeat near the newly replicated TALT1.