A real-time ratiometric fluorescent probe for imaging of SO(2) derivatives in mitochondria of living cells

A real-time ratiometric fluorescent probe (IN-CZ) for highly selective detection of sulfite was designed and synthesized, which is based on modulating the intramolecular charge transfer (ICT) of the hemicyanine dye platform. The mechanism of using the probe is mainly through the Michael addition tha...

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Autores principales: Shi, Junwei, Shu, Wei, Tian, Yong, Wu, Yulong, Jing, Jing, Zhang, Rubo, Zhang, Xiaoling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066615/
https://www.ncbi.nlm.nih.gov/pubmed/35519500
http://dx.doi.org/10.1039/c9ra03207j
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author Shi, Junwei
Shu, Wei
Tian, Yong
Wu, Yulong
Jing, Jing
Zhang, Rubo
Zhang, Xiaoling
author_facet Shi, Junwei
Shu, Wei
Tian, Yong
Wu, Yulong
Jing, Jing
Zhang, Rubo
Zhang, Xiaoling
author_sort Shi, Junwei
collection PubMed
description A real-time ratiometric fluorescent probe (IN-CZ) for highly selective detection of sulfite was designed and synthesized, which is based on modulating the intramolecular charge transfer (ICT) of the hemicyanine dye platform. The mechanism of using the probe is mainly through the Michael addition that occurs between IN-CZ and sulfite with a detection limit of 2.99 × 10(−5) M. IN-CZ displays a fast response (within 1 minute) and is highly selective for SO(3)(2−)/HSO(3)(−) over ROS, biologically relevant ions, biological mercaptans and other reactive species. More importantly, IN-CZ was suitable for ratiometric fluorescence imaging in living cells, by real-time monitoring of SO(3)(2−)/HSO(3)(−) changes in mitochondria targeted in living cells.
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spelling pubmed-90666152022-05-04 A real-time ratiometric fluorescent probe for imaging of SO(2) derivatives in mitochondria of living cells Shi, Junwei Shu, Wei Tian, Yong Wu, Yulong Jing, Jing Zhang, Rubo Zhang, Xiaoling RSC Adv Chemistry A real-time ratiometric fluorescent probe (IN-CZ) for highly selective detection of sulfite was designed and synthesized, which is based on modulating the intramolecular charge transfer (ICT) of the hemicyanine dye platform. The mechanism of using the probe is mainly through the Michael addition that occurs between IN-CZ and sulfite with a detection limit of 2.99 × 10(−5) M. IN-CZ displays a fast response (within 1 minute) and is highly selective for SO(3)(2−)/HSO(3)(−) over ROS, biologically relevant ions, biological mercaptans and other reactive species. More importantly, IN-CZ was suitable for ratiometric fluorescence imaging in living cells, by real-time monitoring of SO(3)(2−)/HSO(3)(−) changes in mitochondria targeted in living cells. The Royal Society of Chemistry 2019-07-18 /pmc/articles/PMC9066615/ /pubmed/35519500 http://dx.doi.org/10.1039/c9ra03207j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Shi, Junwei
Shu, Wei
Tian, Yong
Wu, Yulong
Jing, Jing
Zhang, Rubo
Zhang, Xiaoling
A real-time ratiometric fluorescent probe for imaging of SO(2) derivatives in mitochondria of living cells
title A real-time ratiometric fluorescent probe for imaging of SO(2) derivatives in mitochondria of living cells
title_full A real-time ratiometric fluorescent probe for imaging of SO(2) derivatives in mitochondria of living cells
title_fullStr A real-time ratiometric fluorescent probe for imaging of SO(2) derivatives in mitochondria of living cells
title_full_unstemmed A real-time ratiometric fluorescent probe for imaging of SO(2) derivatives in mitochondria of living cells
title_short A real-time ratiometric fluorescent probe for imaging of SO(2) derivatives in mitochondria of living cells
title_sort real-time ratiometric fluorescent probe for imaging of so(2) derivatives in mitochondria of living cells
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9066615/
https://www.ncbi.nlm.nih.gov/pubmed/35519500
http://dx.doi.org/10.1039/c9ra03207j
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