Impact of Aligner, Normalization Method, and Sequencing Depth on TempO-seq Accuracy

High-throughput transcriptomics has advanced through the introduction of TempO-seq, a targeted alternative to traditional RNA-seq. TempO-seq platforms use 50 nucleotide probes, each specifically designed to target a known transcript, thus allowing for reduced sequencing depth per sample compared wit...

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Detalles Bibliográficos
Autores principales: Everett, Logan J, Mav, Deepak, Phadke, Dhiral P, Balik-Meisner, Michele R, Shah, Ruchir R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2022
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9067045/
https://www.ncbi.nlm.nih.gov/pubmed/35515009
http://dx.doi.org/10.1177/11779322221095216

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