Cargando…

Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China

Foodborne diseases have become a serious havoc, where antimicrobial resistance is throwing significant challenges on daily basis. With the increase of drug-resistant bacteria and food-borne infection associated with Vibrio parahaemolyticus, new and effective strategies were needed to control the eme...

Descripción completa

Detalles Bibliográficos
Autores principales: Tian, Fengjuan, Li, Jing, Hu, Yunjia, Zhao, Feiyang, Ren, Huiying, Pan, Qiang, Nazir, Amina, Li, Fei, Tong, Yigang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9067683/
https://www.ncbi.nlm.nih.gov/pubmed/35507581
http://dx.doi.org/10.1371/journal.pone.0266683
_version_ 1784700058808090624
author Tian, Fengjuan
Li, Jing
Hu, Yunjia
Zhao, Feiyang
Ren, Huiying
Pan, Qiang
Nazir, Amina
Li, Fei
Tong, Yigang
author_facet Tian, Fengjuan
Li, Jing
Hu, Yunjia
Zhao, Feiyang
Ren, Huiying
Pan, Qiang
Nazir, Amina
Li, Fei
Tong, Yigang
author_sort Tian, Fengjuan
collection PubMed
description Foodborne diseases have become a serious havoc, where antimicrobial resistance is throwing significant challenges on daily basis. With the increase of drug-resistant bacteria and food-borne infection associated with Vibrio parahaemolyticus, new and effective strategies were needed to control the emergence of vibriosis. Lytic bacteriophages come up as a promising way to resist the pathogenic population in various applications. In this study, a V. parahaemolyticus specific phage vB_VpS_PG28 was isolated from sewage in the seafood market. Results showed vB_VpS_PG28, is strictly a lytic bacteriophage and has a relatively large burst size of 103 plaque-forming units per infected cell. Comparative genomic and bioinformatic analyses proved that vB_VpS_PG28 is a new bacteriophage that had a homologous relation with Vibrio phages of family Siphoviridae, especially with phage VH2_2019, but transmission electron microscopy of vB_VpS_PG28 morphology characterized its morphology is similar to that of Myoviridae family. In silico analysis indicated that the vB_VpS_PG28 genome consists of 82712 bp (48.08% GC content) encoding 114 putative ORFs without tRNA,and any gene associated with resistance or virulence factors has not been found. The bacteriophage in the present study has shown significant outcomes in order to control bacterial growth under in vitro conditions. Thus, we are suggesting a beneficiary agent against foodborne pathogens. Further, to ensure the safe usage of phage oral toxicity testing is recommended.
format Online
Article
Text
id pubmed-9067683
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-90676832022-05-05 Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China Tian, Fengjuan Li, Jing Hu, Yunjia Zhao, Feiyang Ren, Huiying Pan, Qiang Nazir, Amina Li, Fei Tong, Yigang PLoS One Research Article Foodborne diseases have become a serious havoc, where antimicrobial resistance is throwing significant challenges on daily basis. With the increase of drug-resistant bacteria and food-borne infection associated with Vibrio parahaemolyticus, new and effective strategies were needed to control the emergence of vibriosis. Lytic bacteriophages come up as a promising way to resist the pathogenic population in various applications. In this study, a V. parahaemolyticus specific phage vB_VpS_PG28 was isolated from sewage in the seafood market. Results showed vB_VpS_PG28, is strictly a lytic bacteriophage and has a relatively large burst size of 103 plaque-forming units per infected cell. Comparative genomic and bioinformatic analyses proved that vB_VpS_PG28 is a new bacteriophage that had a homologous relation with Vibrio phages of family Siphoviridae, especially with phage VH2_2019, but transmission electron microscopy of vB_VpS_PG28 morphology characterized its morphology is similar to that of Myoviridae family. In silico analysis indicated that the vB_VpS_PG28 genome consists of 82712 bp (48.08% GC content) encoding 114 putative ORFs without tRNA,and any gene associated with resistance or virulence factors has not been found. The bacteriophage in the present study has shown significant outcomes in order to control bacterial growth under in vitro conditions. Thus, we are suggesting a beneficiary agent against foodborne pathogens. Further, to ensure the safe usage of phage oral toxicity testing is recommended. Public Library of Science 2022-05-04 /pmc/articles/PMC9067683/ /pubmed/35507581 http://dx.doi.org/10.1371/journal.pone.0266683 Text en © 2022 Tian et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Tian, Fengjuan
Li, Jing
Hu, Yunjia
Zhao, Feiyang
Ren, Huiying
Pan, Qiang
Nazir, Amina
Li, Fei
Tong, Yigang
Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China
title Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China
title_full Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China
title_fullStr Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China
title_full_unstemmed Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China
title_short Characterization and complete genome sequence analysis of a newly isolatedphage against Vibrio parahaemolyticus from sick shrimp in Qingdao, China
title_sort characterization and complete genome sequence analysis of a newly isolatedphage against vibrio parahaemolyticus from sick shrimp in qingdao, china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9067683/
https://www.ncbi.nlm.nih.gov/pubmed/35507581
http://dx.doi.org/10.1371/journal.pone.0266683
work_keys_str_mv AT tianfengjuan characterizationandcompletegenomesequenceanalysisofanewlyisolatedphageagainstvibrioparahaemolyticusfromsickshrimpinqingdaochina
AT lijing characterizationandcompletegenomesequenceanalysisofanewlyisolatedphageagainstvibrioparahaemolyticusfromsickshrimpinqingdaochina
AT huyunjia characterizationandcompletegenomesequenceanalysisofanewlyisolatedphageagainstvibrioparahaemolyticusfromsickshrimpinqingdaochina
AT zhaofeiyang characterizationandcompletegenomesequenceanalysisofanewlyisolatedphageagainstvibrioparahaemolyticusfromsickshrimpinqingdaochina
AT renhuiying characterizationandcompletegenomesequenceanalysisofanewlyisolatedphageagainstvibrioparahaemolyticusfromsickshrimpinqingdaochina
AT panqiang characterizationandcompletegenomesequenceanalysisofanewlyisolatedphageagainstvibrioparahaemolyticusfromsickshrimpinqingdaochina
AT naziramina characterizationandcompletegenomesequenceanalysisofanewlyisolatedphageagainstvibrioparahaemolyticusfromsickshrimpinqingdaochina
AT lifei characterizationandcompletegenomesequenceanalysisofanewlyisolatedphageagainstvibrioparahaemolyticusfromsickshrimpinqingdaochina
AT tongyigang characterizationandcompletegenomesequenceanalysisofanewlyisolatedphageagainstvibrioparahaemolyticusfromsickshrimpinqingdaochina