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Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage
Many eukaryotic viruses assemble mature particles within distinct subcellular compartments, but bacteriophages are generally assumed to assemble randomly throughout the host cell cytoplasm. Here, we show that viral particles of Pseudomonas nucleus-forming jumbo phage PhiPA3 assemble into a unique st...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9067925/ https://www.ncbi.nlm.nih.gov/pubmed/35507660 http://dx.doi.org/10.1126/sciadv.abj9670 |
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author | Chaikeeratisak, Vorrapon Khanna, Kanika Nguyen, Katrina T. Egan, MacKennon E. Enustun, Eray Armbruster, Emily Lee, Jina Pogliano, Kit Villa, Elizabeth Pogliano, Joe |
author_facet | Chaikeeratisak, Vorrapon Khanna, Kanika Nguyen, Katrina T. Egan, MacKennon E. Enustun, Eray Armbruster, Emily Lee, Jina Pogliano, Kit Villa, Elizabeth Pogliano, Joe |
author_sort | Chaikeeratisak, Vorrapon |
collection | PubMed |
description | Many eukaryotic viruses assemble mature particles within distinct subcellular compartments, but bacteriophages are generally assumed to assemble randomly throughout the host cell cytoplasm. Here, we show that viral particles of Pseudomonas nucleus-forming jumbo phage PhiPA3 assemble into a unique structure inside cells we term phage bouquets. We show that after capsids complete DNA packaging at the surface of the phage nucleus, tails assemble and attach to capsids, and these particles accumulate over time in a spherical pattern, with tails oriented inward and the heads outward to form bouquets at specific subcellular locations. Bouquets localize at the same fixed distance from the phage nucleus even when it is mispositioned, suggesting an active mechanism for positioning. These results mark the discovery of a pathway for organizing mature viral particles inside bacteria and demonstrate that nucleus-forming jumbo phages, like most eukaryotic viruses, are highly spatially organized during all stages of their lytic cycle. |
format | Online Article Text |
id | pubmed-9067925 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-90679252022-05-13 Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage Chaikeeratisak, Vorrapon Khanna, Kanika Nguyen, Katrina T. Egan, MacKennon E. Enustun, Eray Armbruster, Emily Lee, Jina Pogliano, Kit Villa, Elizabeth Pogliano, Joe Sci Adv Earth, Environmental, Ecological, and Space Sciences Many eukaryotic viruses assemble mature particles within distinct subcellular compartments, but bacteriophages are generally assumed to assemble randomly throughout the host cell cytoplasm. Here, we show that viral particles of Pseudomonas nucleus-forming jumbo phage PhiPA3 assemble into a unique structure inside cells we term phage bouquets. We show that after capsids complete DNA packaging at the surface of the phage nucleus, tails assemble and attach to capsids, and these particles accumulate over time in a spherical pattern, with tails oriented inward and the heads outward to form bouquets at specific subcellular locations. Bouquets localize at the same fixed distance from the phage nucleus even when it is mispositioned, suggesting an active mechanism for positioning. These results mark the discovery of a pathway for organizing mature viral particles inside bacteria and demonstrate that nucleus-forming jumbo phages, like most eukaryotic viruses, are highly spatially organized during all stages of their lytic cycle. American Association for the Advancement of Science 2022-05-04 /pmc/articles/PMC9067925/ /pubmed/35507660 http://dx.doi.org/10.1126/sciadv.abj9670 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Earth, Environmental, Ecological, and Space Sciences Chaikeeratisak, Vorrapon Khanna, Kanika Nguyen, Katrina T. Egan, MacKennon E. Enustun, Eray Armbruster, Emily Lee, Jina Pogliano, Kit Villa, Elizabeth Pogliano, Joe Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage |
title | Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage |
title_full | Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage |
title_fullStr | Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage |
title_full_unstemmed | Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage |
title_short | Subcellular organization of viral particles during maturation of nucleus-forming jumbo phage |
title_sort | subcellular organization of viral particles during maturation of nucleus-forming jumbo phage |
topic | Earth, Environmental, Ecological, and Space Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9067925/ https://www.ncbi.nlm.nih.gov/pubmed/35507660 http://dx.doi.org/10.1126/sciadv.abj9670 |
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