Cargando…

Cysteine metabolic engineering and selective disulfide reduction produce superior antibody-drug-conjugates

Next-generation site-specific cysteine-based antibody–drug-conjugates (ADCs) broaden therapeutic index by precise drug-antibody attachments. However, manufacturing such ADCs for clinical validation requires complex full reduction and reoxidation processes, impacting product quality. To overcome this...

Descripción completa

Detalles Bibliográficos
Autores principales:	Procopio-Melino, Renée, Kotch, Frank W., Prashad, Amar S., Gomes, Jose M., Wang, Wenge, Arve, Bo, Dawdy, Andrew, Chen, Lawrence, Sperry, Justin, Hosselet, Christine, He, Tao, Kriz, Ronald, Lin, Laura, Marquette, Kimberly, Tchistiakova, Lioudmila, Somers, Will, Rouse, Jason C., Zhong, Xiaotian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068625/ https://www.ncbi.nlm.nih.gov/pubmed/35508689 http://dx.doi.org/10.1038/s41598-022-11344-z

Ejemplares similares

Implications of receptor-mediated endocytosis and intracellular trafficking dynamics in the development of antibody drug conjugates
por: Ritchie, Michael, et al.
Publicado: (2013)

A rapid on-line method for mass spectrometric confirmation of a cysteine-conjugated antibody-drug-conjugate structure using multidimensional chromatography
por: Birdsall, Robert E, et al.
Publicado: (2015)

Cysteine oxidation and disulfide formation in the ribosomal exit tunnel
por: Schulte, Linda, et al.
Publicado: (2020)

Optimization of Tubulysin Antibody–Drug Conjugates: A Case Study in Addressing ADC Metabolism
por: Tumey, L. Nathan, et al.
Publicado: (2016)

Disulfide Bond Formation and Cysteine Exclusion in Gram-positive Bacteria
por: Daniels, Robert, et al.
Publicado: (2010)

Diethynyl Phosphinates for Cysteine‐Selective Protein Labeling and Disulfide Rebridging
por: Stieger, Christian E., et al.
Publicado: (2021)

Influence of disulfide bond isoforms on drug conjugation sites in cysteine-linked IgG2 antibody-drug conjugates
por: Liu-Shin, Lily, et al.
Publicado: (2018)

Evaluation of single‐use tangential flow filtration technology for purification of activated polysaccharides used in conjugate vaccine manufacturing
por: Motevalian, Seyed Pouria, et al.
Publicado: (2021)

DISULFIND: a disulfide bonding state and cysteine connectivity prediction server
por: Ceroni, Alessio, et al.
Publicado: (2006)

Residual Dipolar Couplings for Resolving Cysteine Bridges in Disulfide-Rich Peptides
por: Ramanujam, Venkatraman, et al.
Publicado: (2020)

Dithiol Based on l-Cysteine and Cysteamine as a Disulfide-Reducing Agent
por: Bartoccini, Francesca, et al.
Publicado: (2022)

Data on chromatographic isolation of cysteine mixed-disulfide conjugates of Allium thiosulfinates and their role in cellular thiol redox modulation
por: Tocmo, Restituto, et al.
Publicado: (2018)

Cysteine Disulfides (Cys-ss-X) as Sensitive Plasma Biomarkers of Oxidative Stress
por: Fu, Xiaoyun, et al.
Publicado: (2019)

Ginsentides: Cysteine and Glycine-rich Peptides from the Ginseng Family with Unusual Disulfide Connectivity
por: Tam, James P., et al.
Publicado: (2018)

Intermediate Detection in the Casiopeina–Cysteine Interaction Ending in the Disulfide Bond Formation and Copper Reduction
por: Ramírez-Palma, Lillian G., et al.
Publicado: (2021)

Chemoselective cysteine or disulfide modification via single atom substitution in chloromethyl acryl reagents
por: Xu, Lujuan, et al.
Publicado: (2021)

Cysteine Specific Targeting of the Functionally Distinct Peroxiredoxin and Glutaredoxin Proteins by the Investigational Disulfide BNP7787
por: Parker, Aulma R., et al.
Publicado: (2015)

Identification of Sulfenylated Cysteines in Arabidopsis thaliana Proteins Using a Disulfide-Linked Peptide Reporter
por: Wei, Bo, et al.
Publicado: (2020)

Specific chiroptical sensing of cysteine via ultrasound-assisted formation of disulfide bonds in aqueous solution
por: Zhang, Jun-Yao, et al.
Publicado: (2022)

Cysteine-specific protein multi-functionalization and disulfide bridging using 3-bromo-5-methylene pyrrolones
por: Zhang, Yingqian, et al.
Publicado: (2020)

Cysteine [2,4] Disulfide Bond as a New Modifiable Site of α-Conotoxin TxIB
por: Zhang, Baojian, et al.
Publicado: (2021)

Cysteine-Induced Hybridization of 2D Molybdenum Disulfide Films for Efficient and Stable Hydrogen Evolution Reaction
por: Jagminas, Arunas, et al.
Publicado: (2021)

The function of SARS-CoV-2 spike protein is impaired by disulfide-bond disruption with mutation at cysteine-488 and by thiol-reactive N-acetyl-cysteine and glutathione
por: Murae, Mana, et al.
Publicado: (2022)

An Evolutionary View on Disulfide Bond Connectivities Prediction Using Phylogenetic Trees and a Simple Cysteine Mutation Model
por: Raimondi, Daniele, et al.
Publicado: (2015)

Cysteines and Disulfide Bonds as Structure-Forming Units: Insights From Different Domains of Life and the Potential for Characterization by NMR
por: Wiedemann, Christoph, et al.
Publicado: (2020)

Cysteine in cell culture media induces acidic IgG1 species by disrupting the disulfide bond network
por: Prade, Elke, et al.
Publicado: (2020)

Superior Adsorption and Regenerable Dye Adsorbent Based on Flower-Like Molybdenum Disulfide Nanostructure
por: Han, Sancan, et al.
Publicado: (2017)

DBCP: a web server for disulfide bonding connectivity pattern prediction without the prior knowledge of the bonding state of cysteines
por: Lin, Hsuan-Hung, et al.
Publicado: (2010)

Elimination of a Free Cysteine by Creation of a Disulfide Bond Increases the Activity and Stability of Candida boidinii Formate Dehydrogenase
por: Zheng, Junxian, et al.
Publicado: (2016)

Computational analysis of prodomain cysteines in human TGF-β proteins reveals frequent loss of disulfide-dependent regulation in tumors
por: Daly, Samantha M, et al.
Publicado: (2022)

Changes in complementarity-determining regions significantly alter IgG binding to the neonatal Fc receptor (FcRn) and pharmacokinetics
por: Piche-Nicholas, Nicole M., et al.
Publicado: (2017)

Corrigendum: Superior Adsorption and Regenerable Dye Adsorbent Based on Flower-Like Molybdenum Disulfide Nanostructure
por: Han, Sancan, et al.
Publicado: (2017)

Export of a Cysteine-Free Misfolded Secretory Protein from the Endoplasmic Reticulum for Degradation Requires Interaction with Protein Disulfide Isomerase
por: Gillece, Pauline, et al.
Publicado: (1999)

Cysteines and Disulfide-Bridged Macrocyclic Mimics of Teixobactin Analogues and Their Antibacterial Activity Evaluation against Methicillin-Resistant Staphylococcus Aureus (MRSA)
por: Malkawi, Ruba, et al.
Publicado: (2018)

Characterization of a novel cysteine-less Cu/Zn-superoxide dismutase in Paenibacillus lautus missing a conserved disulfide bond
por: Furukawa, Yoshiaki, et al.
Publicado: (2023)

Two Highly Conserved Cysteine Residues in HPV16 L2 Form an Intramolecular Disulfide Bond and Are Critical for Infectivity in Human Keratinocytes
por: Campos, Samuel K., et al.
Publicado: (2009)

N-Acetyl-Cysteine Regenerates Albumin Cys34 by a Thiol-Disulfide Breaking Mechanism: An Explanation of Its Extracellular Antioxidant Activity
por: Altomare, Alessandra, et al.
Publicado: (2020)

Tumor Cell-Surface Binding of Immune Stimulating Polymeric Glyco-Adjuvant via Cysteine-Reactive Pyridyl Disulfide Promotes Antitumor Immunity
por: Slezak, Anna J., et al.
Publicado: (2022)

Homogeneous antibody fragment conjugation by disulfide bridging introduces ‘spinostics'
por: Schumacher, Felix F., et al.
Publicado: (2013)

Cysteine-to-lysine transfer antibody fragment conjugation
por: Forte, Nafsika, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_pgc63c7neesm47nhf0o6kkkp3a