Impact of transforming growth factor beta 1 on normal and thyroid cancer side population cells

PURPOSE: To determine the impact of exogenous transforming growth factor beta 1 (TGF-β1) on side population (SP) cells isolated from normal, papillary thyroid cancer and anaplastic thyroid cancer cell lines and from human thyroid tissues. METHODS: All cell populations were stained with Hoechst 33342...

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Autores principales: Latar, Nani Md, Mahkamova, Kamilla, Elson, Joanna, Karnik, Isha, Sutherland, Rachel, Aspinall, Sebastian, Meeson, Annette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068642/
https://www.ncbi.nlm.nih.gov/pubmed/35118633
http://dx.doi.org/10.1007/s12020-022-02990-4
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author Latar, Nani Md
Mahkamova, Kamilla
Elson, Joanna
Karnik, Isha
Sutherland, Rachel
Aspinall, Sebastian
Meeson, Annette
author_facet Latar, Nani Md
Mahkamova, Kamilla
Elson, Joanna
Karnik, Isha
Sutherland, Rachel
Aspinall, Sebastian
Meeson, Annette
author_sort Latar, Nani Md
collection PubMed
description PURPOSE: To determine the impact of exogenous transforming growth factor beta 1 (TGF-β1) on side population (SP) cells isolated from normal, papillary thyroid cancer and anaplastic thyroid cancer cell lines and from human thyroid tissues. METHODS: All cell populations were stained with Hoechst 33342 and analysed using dual wavelength flow cytometry to identify SP cells. This SP assay was used to assess the impact of TGF-β1 treatment and withdrawal of treatment on SP percentages. Semi-quantitative and quantitative PCR were used for molecular analysis of cells pre and post TGF-β1 treatment. RESULTS: All cell lines expressed mRNA for both TGFB1 and its receptors, as well as showing variable expression of CDH1 and CDH2, with expressing of CDH1 being highest and CDH2 being lowest in the normal cell line. Exposure to exogenous TGF-β1 resulted in a reduction in mRNA expression of ABCG2 compared to controls which was significant between control and treated cancer cell lines. SP cells were isolated from primary human thyroid tissues, with numbers being significantly higher in papillary thyroid cancers. Exposure to TGF-β1 decreased the SP percentage in both thyroid cancer cell lines and completely abrogated these cells in the primary papillary thyroid cancer cultures. On withdrawal of TGF-β1 the SP phenotype was restored in the cancer cell lines and SP percentages increased to above that of untreated cells. CONCLUSIONS: TGF-β1 exposure transiently regulates thyroid cancer SP cells, leading to a reduction in SP percentages, while withdrawal of TGF-β1 results in restoration of the SP phenotype.
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spelling pubmed-90686422022-05-07 Impact of transforming growth factor beta 1 on normal and thyroid cancer side population cells Latar, Nani Md Mahkamova, Kamilla Elson, Joanna Karnik, Isha Sutherland, Rachel Aspinall, Sebastian Meeson, Annette Endocrine Original Article PURPOSE: To determine the impact of exogenous transforming growth factor beta 1 (TGF-β1) on side population (SP) cells isolated from normal, papillary thyroid cancer and anaplastic thyroid cancer cell lines and from human thyroid tissues. METHODS: All cell populations were stained with Hoechst 33342 and analysed using dual wavelength flow cytometry to identify SP cells. This SP assay was used to assess the impact of TGF-β1 treatment and withdrawal of treatment on SP percentages. Semi-quantitative and quantitative PCR were used for molecular analysis of cells pre and post TGF-β1 treatment. RESULTS: All cell lines expressed mRNA for both TGFB1 and its receptors, as well as showing variable expression of CDH1 and CDH2, with expressing of CDH1 being highest and CDH2 being lowest in the normal cell line. Exposure to exogenous TGF-β1 resulted in a reduction in mRNA expression of ABCG2 compared to controls which was significant between control and treated cancer cell lines. SP cells were isolated from primary human thyroid tissues, with numbers being significantly higher in papillary thyroid cancers. Exposure to TGF-β1 decreased the SP percentage in both thyroid cancer cell lines and completely abrogated these cells in the primary papillary thyroid cancer cultures. On withdrawal of TGF-β1 the SP phenotype was restored in the cancer cell lines and SP percentages increased to above that of untreated cells. CONCLUSIONS: TGF-β1 exposure transiently regulates thyroid cancer SP cells, leading to a reduction in SP percentages, while withdrawal of TGF-β1 results in restoration of the SP phenotype. Springer US 2022-02-03 2022 /pmc/articles/PMC9068642/ /pubmed/35118633 http://dx.doi.org/10.1007/s12020-022-02990-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Latar, Nani Md
Mahkamova, Kamilla
Elson, Joanna
Karnik, Isha
Sutherland, Rachel
Aspinall, Sebastian
Meeson, Annette
Impact of transforming growth factor beta 1 on normal and thyroid cancer side population cells
title Impact of transforming growth factor beta 1 on normal and thyroid cancer side population cells
title_full Impact of transforming growth factor beta 1 on normal and thyroid cancer side population cells
title_fullStr Impact of transforming growth factor beta 1 on normal and thyroid cancer side population cells
title_full_unstemmed Impact of transforming growth factor beta 1 on normal and thyroid cancer side population cells
title_short Impact of transforming growth factor beta 1 on normal and thyroid cancer side population cells
title_sort impact of transforming growth factor beta 1 on normal and thyroid cancer side population cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068642/
https://www.ncbi.nlm.nih.gov/pubmed/35118633
http://dx.doi.org/10.1007/s12020-022-02990-4
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