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NMR assignment of non-modified tRNA(Ile) from Escherichia coli
tRNAs are L-shaped RNA molecules of ~ 80 nucleotides that are responsible for decoding the mRNA and for the incorporation of the correct amino acid into the growing peptidyl-chain at the ribosome. They occur in all kingdoms of life and both their functions, and their structure are highly conserved....
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068674/ https://www.ncbi.nlm.nih.gov/pubmed/35275364 http://dx.doi.org/10.1007/s12104-022-10075-6 |
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author | de Jesus, Vanessa Biedenbänder, Thomas Vögele, Jennifer Wöhnert, Jens Fürtig, Boris |
author_facet | de Jesus, Vanessa Biedenbänder, Thomas Vögele, Jennifer Wöhnert, Jens Fürtig, Boris |
author_sort | de Jesus, Vanessa |
collection | PubMed |
description | tRNAs are L-shaped RNA molecules of ~ 80 nucleotides that are responsible for decoding the mRNA and for the incorporation of the correct amino acid into the growing peptidyl-chain at the ribosome. They occur in all kingdoms of life and both their functions, and their structure are highly conserved. The L-shaped tertiary structure is based on a cloverleaf-like secondary structure that consists of four base paired stems connected by three to four loops. The anticodon base triplet, which is complementary to the sequence of the mRNA, resides in the anticodon loop whereas the amino acid is attached to the sequence CCA at the 3′-terminus of the molecule. tRNAs exhibit very stable secondary and tertiary structures and contain up to 10% modified nucleotides. However, their structure and function can also be maintained in the absence of nucleotide modifications. Here, we present the assignments of nucleobase resonances of the non-modified 77 nt tRNA(Ile) from the gram-negative bacterium Escherichia coli. We obtained assignments for all imino resonances visible in the spectra of the tRNA as well as for additional exchangeable and non-exchangeable protons and for heteronuclei of the nucleobases. Based on these assignments we could determine the chemical shift differences between modified and non-modified tRNA(Ile) as a first step towards the analysis of the effect of nucleotide modifications on tRNA’s structure and dynamics. |
format | Online Article Text |
id | pubmed-9068674 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-90686742022-05-07 NMR assignment of non-modified tRNA(Ile) from Escherichia coli de Jesus, Vanessa Biedenbänder, Thomas Vögele, Jennifer Wöhnert, Jens Fürtig, Boris Biomol NMR Assign Article tRNAs are L-shaped RNA molecules of ~ 80 nucleotides that are responsible for decoding the mRNA and for the incorporation of the correct amino acid into the growing peptidyl-chain at the ribosome. They occur in all kingdoms of life and both their functions, and their structure are highly conserved. The L-shaped tertiary structure is based on a cloverleaf-like secondary structure that consists of four base paired stems connected by three to four loops. The anticodon base triplet, which is complementary to the sequence of the mRNA, resides in the anticodon loop whereas the amino acid is attached to the sequence CCA at the 3′-terminus of the molecule. tRNAs exhibit very stable secondary and tertiary structures and contain up to 10% modified nucleotides. However, their structure and function can also be maintained in the absence of nucleotide modifications. Here, we present the assignments of nucleobase resonances of the non-modified 77 nt tRNA(Ile) from the gram-negative bacterium Escherichia coli. We obtained assignments for all imino resonances visible in the spectra of the tRNA as well as for additional exchangeable and non-exchangeable protons and for heteronuclei of the nucleobases. Based on these assignments we could determine the chemical shift differences between modified and non-modified tRNA(Ile) as a first step towards the analysis of the effect of nucleotide modifications on tRNA’s structure and dynamics. Springer Netherlands 2022-03-11 2022 /pmc/articles/PMC9068674/ /pubmed/35275364 http://dx.doi.org/10.1007/s12104-022-10075-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article de Jesus, Vanessa Biedenbänder, Thomas Vögele, Jennifer Wöhnert, Jens Fürtig, Boris NMR assignment of non-modified tRNA(Ile) from Escherichia coli |
title | NMR assignment of non-modified tRNA(Ile) from Escherichia coli |
title_full | NMR assignment of non-modified tRNA(Ile) from Escherichia coli |
title_fullStr | NMR assignment of non-modified tRNA(Ile) from Escherichia coli |
title_full_unstemmed | NMR assignment of non-modified tRNA(Ile) from Escherichia coli |
title_short | NMR assignment of non-modified tRNA(Ile) from Escherichia coli |
title_sort | nmr assignment of non-modified trna(ile) from escherichia coli |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068674/ https://www.ncbi.nlm.nih.gov/pubmed/35275364 http://dx.doi.org/10.1007/s12104-022-10075-6 |
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