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MALDI mass spectrometry imaging workflow for the aquatic model organisms Danio rerio and Daphnia magna

Lipids play various essential roles in the physiology of animals. They are also highly dependent on cellular metabolism or status. It is therefore crucial to understand to which extent animals can stabilize their lipid composition in the presence of external stressors, such as chemicals that are rel...

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Autores principales: Schirmer, Elisabeth, Ritschar, Sven, Ochs, Matthias, Laforsch, Christian, Schuster, Stefan, Römpp, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068711/
https://www.ncbi.nlm.nih.gov/pubmed/35508492
http://dx.doi.org/10.1038/s41598-022-09659-y
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author Schirmer, Elisabeth
Ritschar, Sven
Ochs, Matthias
Laforsch, Christian
Schuster, Stefan
Römpp, Andreas
author_facet Schirmer, Elisabeth
Ritschar, Sven
Ochs, Matthias
Laforsch, Christian
Schuster, Stefan
Römpp, Andreas
author_sort Schirmer, Elisabeth
collection PubMed
description Lipids play various essential roles in the physiology of animals. They are also highly dependent on cellular metabolism or status. It is therefore crucial to understand to which extent animals can stabilize their lipid composition in the presence of external stressors, such as chemicals that are released into the environment. We developed a MALDI MS imaging workflow for two important aquatic model organisms, the zebrafish (Danio rerio) and water flea (Daphnia magna). Owing to the heterogeneous structure of these organisms, developing a suitable sample preparation workflow is a highly non-trivial but crucial part of this work and needs to be established first. Relevant parameters and practical considerations in order to preserve tissue structure and composition in tissue sections are discussed for each application. All measurements were based on high mass accuracy enabling reliable identification of imaged compounds. In zebrafish we demonstrate that a detailed mapping between histology and simultaneously determined lipid composition is possible at various scales, from extended structures such as the brain or gills down to subcellular structures such as a single axon in the central nervous system. For D. magna we present for the first time a MALDI MSI workflow, that demonstrably maintains tissue integrity during cryosectioning of non-preserved samples, and allows the mapping of lipids in the entire body and the brood chamber inside the carapace. In conclusion, the lipid signatures that we were able to detect with our method provide an ideal basis to analyze changes caused by pollutants in two key aquatic model organisms.
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spelling pubmed-90687112022-05-05 MALDI mass spectrometry imaging workflow for the aquatic model organisms Danio rerio and Daphnia magna Schirmer, Elisabeth Ritschar, Sven Ochs, Matthias Laforsch, Christian Schuster, Stefan Römpp, Andreas Sci Rep Article Lipids play various essential roles in the physiology of animals. They are also highly dependent on cellular metabolism or status. It is therefore crucial to understand to which extent animals can stabilize their lipid composition in the presence of external stressors, such as chemicals that are released into the environment. We developed a MALDI MS imaging workflow for two important aquatic model organisms, the zebrafish (Danio rerio) and water flea (Daphnia magna). Owing to the heterogeneous structure of these organisms, developing a suitable sample preparation workflow is a highly non-trivial but crucial part of this work and needs to be established first. Relevant parameters and practical considerations in order to preserve tissue structure and composition in tissue sections are discussed for each application. All measurements were based on high mass accuracy enabling reliable identification of imaged compounds. In zebrafish we demonstrate that a detailed mapping between histology and simultaneously determined lipid composition is possible at various scales, from extended structures such as the brain or gills down to subcellular structures such as a single axon in the central nervous system. For D. magna we present for the first time a MALDI MSI workflow, that demonstrably maintains tissue integrity during cryosectioning of non-preserved samples, and allows the mapping of lipids in the entire body and the brood chamber inside the carapace. In conclusion, the lipid signatures that we were able to detect with our method provide an ideal basis to analyze changes caused by pollutants in two key aquatic model organisms. Nature Publishing Group UK 2022-05-04 /pmc/articles/PMC9068711/ /pubmed/35508492 http://dx.doi.org/10.1038/s41598-022-09659-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Schirmer, Elisabeth
Ritschar, Sven
Ochs, Matthias
Laforsch, Christian
Schuster, Stefan
Römpp, Andreas
MALDI mass spectrometry imaging workflow for the aquatic model organisms Danio rerio and Daphnia magna
title MALDI mass spectrometry imaging workflow for the aquatic model organisms Danio rerio and Daphnia magna
title_full MALDI mass spectrometry imaging workflow for the aquatic model organisms Danio rerio and Daphnia magna
title_fullStr MALDI mass spectrometry imaging workflow for the aquatic model organisms Danio rerio and Daphnia magna
title_full_unstemmed MALDI mass spectrometry imaging workflow for the aquatic model organisms Danio rerio and Daphnia magna
title_short MALDI mass spectrometry imaging workflow for the aquatic model organisms Danio rerio and Daphnia magna
title_sort maldi mass spectrometry imaging workflow for the aquatic model organisms danio rerio and daphnia magna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068711/
https://www.ncbi.nlm.nih.gov/pubmed/35508492
http://dx.doi.org/10.1038/s41598-022-09659-y
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