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Abnormal male reproduction and embryonic development induced by downregulation of a phospholipid fatty acid-introducing enzyme Lpgat1 in zebrafish
Phospholipids in the membrane consist of diverse pairs of fatty acids bound to a glycerol backbone. The biological significance of the diversity, however, remains mostly unclear. Part of this diversity is due to lysophospholipid acyltransferases (LPLATs), which introduce a fatty acid into lysophosph...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068807/ https://www.ncbi.nlm.nih.gov/pubmed/35508627 http://dx.doi.org/10.1038/s41598-022-11002-4 |
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author | Shibata, Takeaki Kawana, Hiroki Nishino, Yuri Ito, Yoshiko Sato, Hiroyasu Onishi, Hirofumi Kano, Kuniyuki Inoue, Asuka Taketomi, Yoshitaka Murakami, Makoto Kofuji, Satoshi Nishina, Hiroshi Miyazawa, Atsuo Kono, Nozomu Aoki, Junken |
author_facet | Shibata, Takeaki Kawana, Hiroki Nishino, Yuri Ito, Yoshiko Sato, Hiroyasu Onishi, Hirofumi Kano, Kuniyuki Inoue, Asuka Taketomi, Yoshitaka Murakami, Makoto Kofuji, Satoshi Nishina, Hiroshi Miyazawa, Atsuo Kono, Nozomu Aoki, Junken |
author_sort | Shibata, Takeaki |
collection | PubMed |
description | Phospholipids in the membrane consist of diverse pairs of fatty acids bound to a glycerol backbone. The biological significance of the diversity, however, remains mostly unclear. Part of this diversity is due to lysophospholipid acyltransferases (LPLATs), which introduce a fatty acid into lysophospholipids. The human genome has 14 LPLATs and most of them are highly conserved in vertebrates. Here, we analyzed the function of one of these enzymes, lysophosphatidylglycerol acyltransferase 1 (Lpgat1), in zebrafish. We found that the reproduction of heterozygous (lpgat1(+/−)) male mutants was abnormal. Crosses between heterozygous males and wild-type females produced many eggs with no obvious cleavage, whereas eggs produced by crosses between heterozygous females and wild-type males cleaved normally. Consistent with this, spermatozoa from heterozygous males had reduced motility and abnormal morphology. We also found that the occurrence of lpgat1 homozygous (lpgat1(−/−)) mutants was far lower than expected. In addition, downregulation of lpgat1 by morpholino antisense oligonucleotides resulted in severe developmental defects. Lipidomic analysis revealed that selective phospholipid species with stearic acid and docosahexaenoic acid were reduced in homozygous larvae and spermatozoa from heterozygotes. These results suggest that the specific phospholipid molecular species produced by Lpgat1 have an essential role in sperm fertilization and in embryonic development. |
format | Online Article Text |
id | pubmed-9068807 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90688072022-05-05 Abnormal male reproduction and embryonic development induced by downregulation of a phospholipid fatty acid-introducing enzyme Lpgat1 in zebrafish Shibata, Takeaki Kawana, Hiroki Nishino, Yuri Ito, Yoshiko Sato, Hiroyasu Onishi, Hirofumi Kano, Kuniyuki Inoue, Asuka Taketomi, Yoshitaka Murakami, Makoto Kofuji, Satoshi Nishina, Hiroshi Miyazawa, Atsuo Kono, Nozomu Aoki, Junken Sci Rep Article Phospholipids in the membrane consist of diverse pairs of fatty acids bound to a glycerol backbone. The biological significance of the diversity, however, remains mostly unclear. Part of this diversity is due to lysophospholipid acyltransferases (LPLATs), which introduce a fatty acid into lysophospholipids. The human genome has 14 LPLATs and most of them are highly conserved in vertebrates. Here, we analyzed the function of one of these enzymes, lysophosphatidylglycerol acyltransferase 1 (Lpgat1), in zebrafish. We found that the reproduction of heterozygous (lpgat1(+/−)) male mutants was abnormal. Crosses between heterozygous males and wild-type females produced many eggs with no obvious cleavage, whereas eggs produced by crosses between heterozygous females and wild-type males cleaved normally. Consistent with this, spermatozoa from heterozygous males had reduced motility and abnormal morphology. We also found that the occurrence of lpgat1 homozygous (lpgat1(−/−)) mutants was far lower than expected. In addition, downregulation of lpgat1 by morpholino antisense oligonucleotides resulted in severe developmental defects. Lipidomic analysis revealed that selective phospholipid species with stearic acid and docosahexaenoic acid were reduced in homozygous larvae and spermatozoa from heterozygotes. These results suggest that the specific phospholipid molecular species produced by Lpgat1 have an essential role in sperm fertilization and in embryonic development. Nature Publishing Group UK 2022-05-04 /pmc/articles/PMC9068807/ /pubmed/35508627 http://dx.doi.org/10.1038/s41598-022-11002-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Shibata, Takeaki Kawana, Hiroki Nishino, Yuri Ito, Yoshiko Sato, Hiroyasu Onishi, Hirofumi Kano, Kuniyuki Inoue, Asuka Taketomi, Yoshitaka Murakami, Makoto Kofuji, Satoshi Nishina, Hiroshi Miyazawa, Atsuo Kono, Nozomu Aoki, Junken Abnormal male reproduction and embryonic development induced by downregulation of a phospholipid fatty acid-introducing enzyme Lpgat1 in zebrafish |
title | Abnormal male reproduction and embryonic development induced by downregulation of a phospholipid fatty acid-introducing enzyme Lpgat1 in zebrafish |
title_full | Abnormal male reproduction and embryonic development induced by downregulation of a phospholipid fatty acid-introducing enzyme Lpgat1 in zebrafish |
title_fullStr | Abnormal male reproduction and embryonic development induced by downregulation of a phospholipid fatty acid-introducing enzyme Lpgat1 in zebrafish |
title_full_unstemmed | Abnormal male reproduction and embryonic development induced by downregulation of a phospholipid fatty acid-introducing enzyme Lpgat1 in zebrafish |
title_short | Abnormal male reproduction and embryonic development induced by downregulation of a phospholipid fatty acid-introducing enzyme Lpgat1 in zebrafish |
title_sort | abnormal male reproduction and embryonic development induced by downregulation of a phospholipid fatty acid-introducing enzyme lpgat1 in zebrafish |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068807/ https://www.ncbi.nlm.nih.gov/pubmed/35508627 http://dx.doi.org/10.1038/s41598-022-11002-4 |
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