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Remodeling of Hepatocyte Mitochondrial Metabolism and De Novo Lipogenesis During the Embryonic-to-Neonatal Transition in Chickens

Embryonic-to-neonatal development in chicken is characterized by high rates of lipid oxidation in the late-term embryonic liver and high rates of de novo lipogenesis in the neonatal liver. This rapid remodeling of hepatic mitochondrial and cytoplasmic networks occurs without symptoms of hepatocellul...

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Autores principales: Surugihalli, Chaitra, Farley, Linda S., Beckford, Ronique C., Kamkrathok, Boonyarit, Liu, Hsiao-Ching, Muralidaran, Vaishna, Patel, Kruti, Porter, Tom E., Sunny, Nishanth E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068877/
https://www.ncbi.nlm.nih.gov/pubmed/35530509
http://dx.doi.org/10.3389/fphys.2022.870451
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author Surugihalli, Chaitra
Farley, Linda S.
Beckford, Ronique C.
Kamkrathok, Boonyarit
Liu, Hsiao-Ching
Muralidaran, Vaishna
Patel, Kruti
Porter, Tom E.
Sunny, Nishanth E.
author_facet Surugihalli, Chaitra
Farley, Linda S.
Beckford, Ronique C.
Kamkrathok, Boonyarit
Liu, Hsiao-Ching
Muralidaran, Vaishna
Patel, Kruti
Porter, Tom E.
Sunny, Nishanth E.
author_sort Surugihalli, Chaitra
collection PubMed
description Embryonic-to-neonatal development in chicken is characterized by high rates of lipid oxidation in the late-term embryonic liver and high rates of de novo lipogenesis in the neonatal liver. This rapid remodeling of hepatic mitochondrial and cytoplasmic networks occurs without symptoms of hepatocellular stress. Our objective was to characterize the metabolic phenotype of the embryonic and neonatal liver and explore whether these metabolic signatures are preserved in primary cultured hepatocytes. Plasma and liver metabolites were profiled using mass spectrometry based metabolomics on embryonic day 18 (ed18) and neonatal day 3 (nd3). Hepatocytes from ed18 and nd3 were isolated and cultured, and treated with insulin, glucagon, growth hormone and corticosterone to define hormonal responsiveness and determine their impacts on mitochondrial metabolism and lipogenesis. Metabolic profiling illustrated the clear transition from the embryonic liver relying on lipid oxidation to the neonatal liver upregulating de novo lipogenesis. This metabolic phenotype was conserved in the isolated hepatocytes from the embryos and the neonates. Cultured hepatocytes from the neonatal liver also maintained a robust response to insulin and glucagon, as evidenced by their contradictory effects on lipid oxidation and lipogenesis. In summary, primary hepatocytes from the embryonic and neonatal chicken could be a valuable tool to investigate mechanisms regulating hepatic mitochondrial metabolism and de novo lipogenesis.
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spelling pubmed-90688772022-05-05 Remodeling of Hepatocyte Mitochondrial Metabolism and De Novo Lipogenesis During the Embryonic-to-Neonatal Transition in Chickens Surugihalli, Chaitra Farley, Linda S. Beckford, Ronique C. Kamkrathok, Boonyarit Liu, Hsiao-Ching Muralidaran, Vaishna Patel, Kruti Porter, Tom E. Sunny, Nishanth E. Front Physiol Physiology Embryonic-to-neonatal development in chicken is characterized by high rates of lipid oxidation in the late-term embryonic liver and high rates of de novo lipogenesis in the neonatal liver. This rapid remodeling of hepatic mitochondrial and cytoplasmic networks occurs without symptoms of hepatocellular stress. Our objective was to characterize the metabolic phenotype of the embryonic and neonatal liver and explore whether these metabolic signatures are preserved in primary cultured hepatocytes. Plasma and liver metabolites were profiled using mass spectrometry based metabolomics on embryonic day 18 (ed18) and neonatal day 3 (nd3). Hepatocytes from ed18 and nd3 were isolated and cultured, and treated with insulin, glucagon, growth hormone and corticosterone to define hormonal responsiveness and determine their impacts on mitochondrial metabolism and lipogenesis. Metabolic profiling illustrated the clear transition from the embryonic liver relying on lipid oxidation to the neonatal liver upregulating de novo lipogenesis. This metabolic phenotype was conserved in the isolated hepatocytes from the embryos and the neonates. Cultured hepatocytes from the neonatal liver also maintained a robust response to insulin and glucagon, as evidenced by their contradictory effects on lipid oxidation and lipogenesis. In summary, primary hepatocytes from the embryonic and neonatal chicken could be a valuable tool to investigate mechanisms regulating hepatic mitochondrial metabolism and de novo lipogenesis. Frontiers Media S.A. 2022-04-21 /pmc/articles/PMC9068877/ /pubmed/35530509 http://dx.doi.org/10.3389/fphys.2022.870451 Text en Copyright © 2022 Surugihalli, Farley, Beckford, Kamkrathok, Liu, Muralidaran, Patel, Porter and Sunny. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Surugihalli, Chaitra
Farley, Linda S.
Beckford, Ronique C.
Kamkrathok, Boonyarit
Liu, Hsiao-Ching
Muralidaran, Vaishna
Patel, Kruti
Porter, Tom E.
Sunny, Nishanth E.
Remodeling of Hepatocyte Mitochondrial Metabolism and De Novo Lipogenesis During the Embryonic-to-Neonatal Transition in Chickens
title Remodeling of Hepatocyte Mitochondrial Metabolism and De Novo Lipogenesis During the Embryonic-to-Neonatal Transition in Chickens
title_full Remodeling of Hepatocyte Mitochondrial Metabolism and De Novo Lipogenesis During the Embryonic-to-Neonatal Transition in Chickens
title_fullStr Remodeling of Hepatocyte Mitochondrial Metabolism and De Novo Lipogenesis During the Embryonic-to-Neonatal Transition in Chickens
title_full_unstemmed Remodeling of Hepatocyte Mitochondrial Metabolism and De Novo Lipogenesis During the Embryonic-to-Neonatal Transition in Chickens
title_short Remodeling of Hepatocyte Mitochondrial Metabolism and De Novo Lipogenesis During the Embryonic-to-Neonatal Transition in Chickens
title_sort remodeling of hepatocyte mitochondrial metabolism and de novo lipogenesis during the embryonic-to-neonatal transition in chickens
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068877/
https://www.ncbi.nlm.nih.gov/pubmed/35530509
http://dx.doi.org/10.3389/fphys.2022.870451
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