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Condensed Mitochondria Assemble Into the Acrosomal Matrix During Spermiogenesis

Mammalian spermatogenesis is associated with the transient appearance of condensed mitochondria, a singularity of germ cells with unknown function. Using proteomic analysis, respirometry, and electron microscopy with tomography, we studied the development of condensed mitochondria. Condensed mitocho...

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Autores principales: Ren, Mindong, Xu, Yang, Phoon, Colin K. L., Erdjument-Bromage, Hediye, Neubert, Thomas A., Rajan, Sujith, Hussain, M. Mahmood, Schlame, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068883/
https://www.ncbi.nlm.nih.gov/pubmed/35531097
http://dx.doi.org/10.3389/fcell.2022.867175
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author Ren, Mindong
Xu, Yang
Phoon, Colin K. L.
Erdjument-Bromage, Hediye
Neubert, Thomas A.
Rajan, Sujith
Hussain, M. Mahmood
Schlame, Michael
author_facet Ren, Mindong
Xu, Yang
Phoon, Colin K. L.
Erdjument-Bromage, Hediye
Neubert, Thomas A.
Rajan, Sujith
Hussain, M. Mahmood
Schlame, Michael
author_sort Ren, Mindong
collection PubMed
description Mammalian spermatogenesis is associated with the transient appearance of condensed mitochondria, a singularity of germ cells with unknown function. Using proteomic analysis, respirometry, and electron microscopy with tomography, we studied the development of condensed mitochondria. Condensed mitochondria arose from orthodox mitochondria during meiosis by progressive contraction of the matrix space, which was accompanied by an initial expansion and a subsequent reduction of the surface area of the inner membrane. Compared to orthodox mitochondria, condensed mitochondria respired more actively, had a higher concentration of respiratory enzymes and supercomplexes, and contained more proteins involved in protein import and expression. After the completion of meiosis, the abundance of condensed mitochondria declined, which coincided with the onset of the biogenesis of acrosomes. Immuno-electron microscopy and the analysis of sub-cellular fractions suggested that condensed mitochondria or their fragments were translocated into the lumen of the acrosome. Thus, it seems condensed mitochondria are formed from orthodox mitochondria by extensive transformations in order to support the formation of the acrosomal matrix.
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spelling pubmed-90688832022-05-05 Condensed Mitochondria Assemble Into the Acrosomal Matrix During Spermiogenesis Ren, Mindong Xu, Yang Phoon, Colin K. L. Erdjument-Bromage, Hediye Neubert, Thomas A. Rajan, Sujith Hussain, M. Mahmood Schlame, Michael Front Cell Dev Biol Cell and Developmental Biology Mammalian spermatogenesis is associated with the transient appearance of condensed mitochondria, a singularity of germ cells with unknown function. Using proteomic analysis, respirometry, and electron microscopy with tomography, we studied the development of condensed mitochondria. Condensed mitochondria arose from orthodox mitochondria during meiosis by progressive contraction of the matrix space, which was accompanied by an initial expansion and a subsequent reduction of the surface area of the inner membrane. Compared to orthodox mitochondria, condensed mitochondria respired more actively, had a higher concentration of respiratory enzymes and supercomplexes, and contained more proteins involved in protein import and expression. After the completion of meiosis, the abundance of condensed mitochondria declined, which coincided with the onset of the biogenesis of acrosomes. Immuno-electron microscopy and the analysis of sub-cellular fractions suggested that condensed mitochondria or their fragments were translocated into the lumen of the acrosome. Thus, it seems condensed mitochondria are formed from orthodox mitochondria by extensive transformations in order to support the formation of the acrosomal matrix. Frontiers Media S.A. 2022-04-21 /pmc/articles/PMC9068883/ /pubmed/35531097 http://dx.doi.org/10.3389/fcell.2022.867175 Text en Copyright © 2022 Ren, Xu, Phoon, Erdjument-Bromage, Neubert, Rajan, Hussain and Schlame. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Ren, Mindong
Xu, Yang
Phoon, Colin K. L.
Erdjument-Bromage, Hediye
Neubert, Thomas A.
Rajan, Sujith
Hussain, M. Mahmood
Schlame, Michael
Condensed Mitochondria Assemble Into the Acrosomal Matrix During Spermiogenesis
title Condensed Mitochondria Assemble Into the Acrosomal Matrix During Spermiogenesis
title_full Condensed Mitochondria Assemble Into the Acrosomal Matrix During Spermiogenesis
title_fullStr Condensed Mitochondria Assemble Into the Acrosomal Matrix During Spermiogenesis
title_full_unstemmed Condensed Mitochondria Assemble Into the Acrosomal Matrix During Spermiogenesis
title_short Condensed Mitochondria Assemble Into the Acrosomal Matrix During Spermiogenesis
title_sort condensed mitochondria assemble into the acrosomal matrix during spermiogenesis
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9068883/
https://www.ncbi.nlm.nih.gov/pubmed/35531097
http://dx.doi.org/10.3389/fcell.2022.867175
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