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Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum

We characterized a negative sense single-stranded RNA mycovirus, Fusarium oxysporum mymonavirus 1 (FoMyV1), isolated from the phytopathogenic fungus Fusarium oxysporum. The genome of FoMyV1 is 10,114 nt, including five open reading frames (ORFs1–5) that are non-overlapping and linearly arranged. The...

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Autores principales: Wang, Jing, Li, Chengjun, Song, Pengyu, Qiu, Rui, Song, Ruifang, Li, Xiaojie, Ni, Yunxia, Zhao, Hui, Liu, Hongyan, Li, Shujun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069137/
https://www.ncbi.nlm.nih.gov/pubmed/35531277
http://dx.doi.org/10.3389/fmicb.2022.870204
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author Wang, Jing
Li, Chengjun
Song, Pengyu
Qiu, Rui
Song, Ruifang
Li, Xiaojie
Ni, Yunxia
Zhao, Hui
Liu, Hongyan
Li, Shujun
author_facet Wang, Jing
Li, Chengjun
Song, Pengyu
Qiu, Rui
Song, Ruifang
Li, Xiaojie
Ni, Yunxia
Zhao, Hui
Liu, Hongyan
Li, Shujun
author_sort Wang, Jing
collection PubMed
description We characterized a negative sense single-stranded RNA mycovirus, Fusarium oxysporum mymonavirus 1 (FoMyV1), isolated from the phytopathogenic fungus Fusarium oxysporum. The genome of FoMyV1 is 10,114 nt, including five open reading frames (ORFs1–5) that are non-overlapping and linearly arranged. The largest, ORF5, encodes a large polypeptide L containing a conserved regions corresponding to Mononegavirales RNA-dependent RNA polymerase and mRNA-capping enzyme region V; the putative functions of the remaining four ORFs are unknown. The L protein encoded by ORF5 shared a high amino acid identity of 65% with that of Hubei rhabdo-like virus 4, a mymonavirus that associated with arthropods. However, the L protein of FoMyV1 also showed amino acid similarity (27–36%) with proteins of mynonaviruses that infect the phytopathogenic fungi Sclerotinia sclerotiorum and Botrytis cineaea. Phylogenetic analysis based on L protein showed that FoMyV1 is clustered with the members of the genus Hubramonavirus in the family Mymonaviridae. Moreover, we found that FoMyV1 could successfully transfer by hyphal anastomosis to a virus-free strain. FoMyV1 reduced the vegetative growth and conidium production of its fungal host but did not alter its virulence. To the best of our knowledge, this is not only the first mymonavirus described in the species F. oxysporum, but also the first Hubramonavirus species found to infect a fungus. However, the incidence of FoMyV1 infections in the tested F. oxysporum strains was only 1%.
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spelling pubmed-90691372022-05-05 Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum Wang, Jing Li, Chengjun Song, Pengyu Qiu, Rui Song, Ruifang Li, Xiaojie Ni, Yunxia Zhao, Hui Liu, Hongyan Li, Shujun Front Microbiol Microbiology We characterized a negative sense single-stranded RNA mycovirus, Fusarium oxysporum mymonavirus 1 (FoMyV1), isolated from the phytopathogenic fungus Fusarium oxysporum. The genome of FoMyV1 is 10,114 nt, including five open reading frames (ORFs1–5) that are non-overlapping and linearly arranged. The largest, ORF5, encodes a large polypeptide L containing a conserved regions corresponding to Mononegavirales RNA-dependent RNA polymerase and mRNA-capping enzyme region V; the putative functions of the remaining four ORFs are unknown. The L protein encoded by ORF5 shared a high amino acid identity of 65% with that of Hubei rhabdo-like virus 4, a mymonavirus that associated with arthropods. However, the L protein of FoMyV1 also showed amino acid similarity (27–36%) with proteins of mynonaviruses that infect the phytopathogenic fungi Sclerotinia sclerotiorum and Botrytis cineaea. Phylogenetic analysis based on L protein showed that FoMyV1 is clustered with the members of the genus Hubramonavirus in the family Mymonaviridae. Moreover, we found that FoMyV1 could successfully transfer by hyphal anastomosis to a virus-free strain. FoMyV1 reduced the vegetative growth and conidium production of its fungal host but did not alter its virulence. To the best of our knowledge, this is not only the first mymonavirus described in the species F. oxysporum, but also the first Hubramonavirus species found to infect a fungus. However, the incidence of FoMyV1 infections in the tested F. oxysporum strains was only 1%. Frontiers Media S.A. 2022-04-21 /pmc/articles/PMC9069137/ /pubmed/35531277 http://dx.doi.org/10.3389/fmicb.2022.870204 Text en Copyright © 2022 Wang, Li, Song, Qiu, Song, Li, Ni, Zhao, Liu and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Jing
Li, Chengjun
Song, Pengyu
Qiu, Rui
Song, Ruifang
Li, Xiaojie
Ni, Yunxia
Zhao, Hui
Liu, Hongyan
Li, Shujun
Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum
title Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum
title_full Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum
title_fullStr Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum
title_full_unstemmed Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum
title_short Molecular and Biological Characterization of the First Mymonavirus Identified in Fusarium oxysporum
title_sort molecular and biological characterization of the first mymonavirus identified in fusarium oxysporum
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069137/
https://www.ncbi.nlm.nih.gov/pubmed/35531277
http://dx.doi.org/10.3389/fmicb.2022.870204
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