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Single‐cell profiling reveals novel cellular heterogeneity of monocytes during Hymenoptera venom allergy
BACKGROUND: Hymenoptera stings can induce dysregulated inflammation and immediate hypersensitivity reactions including anaphylaxis. However, the molecular mechanisms underlying peripheral immune responses during Hymenoptera venom allergy (HVA) remain elusive. METHODS: Here we determined the single‐c...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069361/ https://www.ncbi.nlm.nih.gov/pubmed/35540108 http://dx.doi.org/10.1002/clt2.12151 |
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author | Chao, Wen‐Cheng Liao, Wen‐Ting Wang, Jing‐Rong Sung, Hsiao‐Ni Chen, Hsin‐Hua Lin, Fang‐Ping Huang, Jou‐Yu Liu, Kuan‐Ting Ko, Tai‐Ming |
author_facet | Chao, Wen‐Cheng Liao, Wen‐Ting Wang, Jing‐Rong Sung, Hsiao‐Ni Chen, Hsin‐Hua Lin, Fang‐Ping Huang, Jou‐Yu Liu, Kuan‐Ting Ko, Tai‐Ming |
author_sort | Chao, Wen‐Cheng |
collection | PubMed |
description | BACKGROUND: Hymenoptera stings can induce dysregulated inflammation and immediate hypersensitivity reactions including anaphylaxis. However, the molecular mechanisms underlying peripheral immune responses during Hymenoptera venom allergy (HVA) remain elusive. METHODS: Here we determined the single‐cell transcriptomic profiling from highly heterogeneous peripheral blood cells in patients with HVA through unbiased single‐cell RNA sequencing and multiple models of computational analyses. RESULTS: Through clustering analysis by uniform manifold approximation and projection, we revealed an increased number of monocytes in the acute phase and identified innate immune responses, leukocyte activation, and cellular detoxification as the main involved biological processes. We used filter analysis to identify that CLU that encodes clusterin was highly expressed in monocytes, and the co‐expressed genes of CLU further supported the key role of monocyte. We further used pseudo‐temporal ordering of cells and scRNA velocity analysis to delineate disease‐associated monocyte lineages and states in patients with HVA. CONCLUSIONS: Our comprehensive molecular profiling of blood samples from patients with HVA revealed previously unknown molecular changes, providing important insights into the mechanism of venom allergy and potential therapeutic targets. |
format | Online Article Text |
id | pubmed-9069361 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90693612022-05-09 Single‐cell profiling reveals novel cellular heterogeneity of monocytes during Hymenoptera venom allergy Chao, Wen‐Cheng Liao, Wen‐Ting Wang, Jing‐Rong Sung, Hsiao‐Ni Chen, Hsin‐Hua Lin, Fang‐Ping Huang, Jou‐Yu Liu, Kuan‐Ting Ko, Tai‐Ming Clin Transl Allergy Letter BACKGROUND: Hymenoptera stings can induce dysregulated inflammation and immediate hypersensitivity reactions including anaphylaxis. However, the molecular mechanisms underlying peripheral immune responses during Hymenoptera venom allergy (HVA) remain elusive. METHODS: Here we determined the single‐cell transcriptomic profiling from highly heterogeneous peripheral blood cells in patients with HVA through unbiased single‐cell RNA sequencing and multiple models of computational analyses. RESULTS: Through clustering analysis by uniform manifold approximation and projection, we revealed an increased number of monocytes in the acute phase and identified innate immune responses, leukocyte activation, and cellular detoxification as the main involved biological processes. We used filter analysis to identify that CLU that encodes clusterin was highly expressed in monocytes, and the co‐expressed genes of CLU further supported the key role of monocyte. We further used pseudo‐temporal ordering of cells and scRNA velocity analysis to delineate disease‐associated monocyte lineages and states in patients with HVA. CONCLUSIONS: Our comprehensive molecular profiling of blood samples from patients with HVA revealed previously unknown molecular changes, providing important insights into the mechanism of venom allergy and potential therapeutic targets. John Wiley and Sons Inc. 2022-05-05 /pmc/articles/PMC9069361/ /pubmed/35540108 http://dx.doi.org/10.1002/clt2.12151 Text en © 2022 The Authors. Clinical and Translational Allergy published by John Wiley & Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Letter Chao, Wen‐Cheng Liao, Wen‐Ting Wang, Jing‐Rong Sung, Hsiao‐Ni Chen, Hsin‐Hua Lin, Fang‐Ping Huang, Jou‐Yu Liu, Kuan‐Ting Ko, Tai‐Ming Single‐cell profiling reveals novel cellular heterogeneity of monocytes during Hymenoptera venom allergy |
title | Single‐cell profiling reveals novel cellular heterogeneity of monocytes during Hymenoptera venom allergy |
title_full | Single‐cell profiling reveals novel cellular heterogeneity of monocytes during Hymenoptera venom allergy |
title_fullStr | Single‐cell profiling reveals novel cellular heterogeneity of monocytes during Hymenoptera venom allergy |
title_full_unstemmed | Single‐cell profiling reveals novel cellular heterogeneity of monocytes during Hymenoptera venom allergy |
title_short | Single‐cell profiling reveals novel cellular heterogeneity of monocytes during Hymenoptera venom allergy |
title_sort | single‐cell profiling reveals novel cellular heterogeneity of monocytes during hymenoptera venom allergy |
topic | Letter |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069361/ https://www.ncbi.nlm.nih.gov/pubmed/35540108 http://dx.doi.org/10.1002/clt2.12151 |
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