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Raman cell imaging with boron cluster molecules conjugated with biomolecules

Raman spectroscopic measurements and theoretical calculation revealed that the Raman bands corresponding to the B–H stretching vibrations of two types of simple icosahedral boron clusters, ortho-carborane 3 and closo-dodecaborate 4 appeared at approximately 2450–2700 cm(−1), and did not overlap with...

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Autores principales: Mochizuki, Masahito, Sato, Shinichi, Asatyas, Syifa, Leśnikowski, Zbigniew J., Hayashi, Tomohiro, Nakamura, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069464/
https://www.ncbi.nlm.nih.gov/pubmed/35530627
http://dx.doi.org/10.1039/c9ra04228h
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author Mochizuki, Masahito
Sato, Shinichi
Asatyas, Syifa
Leśnikowski, Zbigniew J.
Hayashi, Tomohiro
Nakamura, Hiroyuki
author_facet Mochizuki, Masahito
Sato, Shinichi
Asatyas, Syifa
Leśnikowski, Zbigniew J.
Hayashi, Tomohiro
Nakamura, Hiroyuki
author_sort Mochizuki, Masahito
collection PubMed
description Raman spectroscopic measurements and theoretical calculation revealed that the Raman bands corresponding to the B–H stretching vibrations of two types of simple icosahedral boron clusters, ortho-carborane 3 and closo-dodecaborate 4 appeared at approximately 2450–2700 cm(−1), and did not overlap with those of cellular components. Although ortho-carborane 3 possesses a possible property as a Raman probe, it was difficult to measure Raman imaging in the cell due to its poor water solubility. In fact, ortho-carborane derivative 6, which internally has an alkyne moiety, exhibited very weak Raman signals of the C[triple bond, length as m-dash]C stretching and the B–H stretching vibrations were barely detected at a 400 ppm boron concentration in HeLa cells. In contrast, closo-dodecaborate derivatives such as BSH (5) were found to be a potential Raman imaging probe cluster for target molecules in the cell. BSH-conjugated cholesterol 7 (BSH-Chol) was synthesized and used in Raman imaging in cells. Raman imaging and spectral analysis revealed that BSH-based Raman tags provide a versatile platform for quantitative Raman imaging.
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spelling pubmed-90694642022-05-05 Raman cell imaging with boron cluster molecules conjugated with biomolecules Mochizuki, Masahito Sato, Shinichi Asatyas, Syifa Leśnikowski, Zbigniew J. Hayashi, Tomohiro Nakamura, Hiroyuki RSC Adv Chemistry Raman spectroscopic measurements and theoretical calculation revealed that the Raman bands corresponding to the B–H stretching vibrations of two types of simple icosahedral boron clusters, ortho-carborane 3 and closo-dodecaborate 4 appeared at approximately 2450–2700 cm(−1), and did not overlap with those of cellular components. Although ortho-carborane 3 possesses a possible property as a Raman probe, it was difficult to measure Raman imaging in the cell due to its poor water solubility. In fact, ortho-carborane derivative 6, which internally has an alkyne moiety, exhibited very weak Raman signals of the C[triple bond, length as m-dash]C stretching and the B–H stretching vibrations were barely detected at a 400 ppm boron concentration in HeLa cells. In contrast, closo-dodecaborate derivatives such as BSH (5) were found to be a potential Raman imaging probe cluster for target molecules in the cell. BSH-conjugated cholesterol 7 (BSH-Chol) was synthesized and used in Raman imaging in cells. Raman imaging and spectral analysis revealed that BSH-based Raman tags provide a versatile platform for quantitative Raman imaging. The Royal Society of Chemistry 2019-08-02 /pmc/articles/PMC9069464/ /pubmed/35530627 http://dx.doi.org/10.1039/c9ra04228h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Mochizuki, Masahito
Sato, Shinichi
Asatyas, Syifa
Leśnikowski, Zbigniew J.
Hayashi, Tomohiro
Nakamura, Hiroyuki
Raman cell imaging with boron cluster molecules conjugated with biomolecules
title Raman cell imaging with boron cluster molecules conjugated with biomolecules
title_full Raman cell imaging with boron cluster molecules conjugated with biomolecules
title_fullStr Raman cell imaging with boron cluster molecules conjugated with biomolecules
title_full_unstemmed Raman cell imaging with boron cluster molecules conjugated with biomolecules
title_short Raman cell imaging with boron cluster molecules conjugated with biomolecules
title_sort raman cell imaging with boron cluster molecules conjugated with biomolecules
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069464/
https://www.ncbi.nlm.nih.gov/pubmed/35530627
http://dx.doi.org/10.1039/c9ra04228h
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