An acetylcholinesterase biosensor with high stability and sensitivity based on silver nanowire–graphene–TiO(2) for the detection of organophosphate pesticides

An electrochemical acetylcholinesterase biosensor based on silver nanowire, graphene, TiO(2) sol–gel, chitosan and acetylcholinesterase has been fabricated successfully for the detection of organophosphate pesticides. The outstanding electrical properties of silver nanowires and graphene, and moreov...

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Autores principales: Zhang, Jianhua, Wang, Bo, Li, Yiru, Shu, Wenhao, Hu, Huaying, Yang, Lianqiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069864/
https://www.ncbi.nlm.nih.gov/pubmed/35528694
http://dx.doi.org/10.1039/c9ra02140j
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author Zhang, Jianhua
Wang, Bo
Li, Yiru
Shu, Wenhao
Hu, Huaying
Yang, Lianqiao
author_facet Zhang, Jianhua
Wang, Bo
Li, Yiru
Shu, Wenhao
Hu, Huaying
Yang, Lianqiao
author_sort Zhang, Jianhua
collection PubMed
description An electrochemical acetylcholinesterase biosensor based on silver nanowire, graphene, TiO(2) sol–gel, chitosan and acetylcholinesterase has been fabricated successfully for the detection of organophosphate pesticides. The outstanding electrical properties of silver nanowires and graphene, and moreover the self-assembly of these two nanomaterials make the biosensor highly sensitive. Simultaneously, the immobilization efficiency of the enzyme is greatly improved by the action of the TiO(2) fixed matrix. Under optimum conditions, the biosensor exhibited excellent performance for the detection of dichlorvos with a linearity in the range of 0.036 μM to 22.63 μM and the detection limit was found to be 7.4 nM. The biosensor was highly reproducible and stable during detection and storage.
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spelling pubmed-90698642022-05-05 An acetylcholinesterase biosensor with high stability and sensitivity based on silver nanowire–graphene–TiO(2) for the detection of organophosphate pesticides Zhang, Jianhua Wang, Bo Li, Yiru Shu, Wenhao Hu, Huaying Yang, Lianqiao RSC Adv Chemistry An electrochemical acetylcholinesterase biosensor based on silver nanowire, graphene, TiO(2) sol–gel, chitosan and acetylcholinesterase has been fabricated successfully for the detection of organophosphate pesticides. The outstanding electrical properties of silver nanowires and graphene, and moreover the self-assembly of these two nanomaterials make the biosensor highly sensitive. Simultaneously, the immobilization efficiency of the enzyme is greatly improved by the action of the TiO(2) fixed matrix. Under optimum conditions, the biosensor exhibited excellent performance for the detection of dichlorvos with a linearity in the range of 0.036 μM to 22.63 μM and the detection limit was found to be 7.4 nM. The biosensor was highly reproducible and stable during detection and storage. The Royal Society of Chemistry 2019-08-13 /pmc/articles/PMC9069864/ /pubmed/35528694 http://dx.doi.org/10.1039/c9ra02140j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Zhang, Jianhua
Wang, Bo
Li, Yiru
Shu, Wenhao
Hu, Huaying
Yang, Lianqiao
An acetylcholinesterase biosensor with high stability and sensitivity based on silver nanowire–graphene–TiO(2) for the detection of organophosphate pesticides
title An acetylcholinesterase biosensor with high stability and sensitivity based on silver nanowire–graphene–TiO(2) for the detection of organophosphate pesticides
title_full An acetylcholinesterase biosensor with high stability and sensitivity based on silver nanowire–graphene–TiO(2) for the detection of organophosphate pesticides
title_fullStr An acetylcholinesterase biosensor with high stability and sensitivity based on silver nanowire–graphene–TiO(2) for the detection of organophosphate pesticides
title_full_unstemmed An acetylcholinesterase biosensor with high stability and sensitivity based on silver nanowire–graphene–TiO(2) for the detection of organophosphate pesticides
title_short An acetylcholinesterase biosensor with high stability and sensitivity based on silver nanowire–graphene–TiO(2) for the detection of organophosphate pesticides
title_sort acetylcholinesterase biosensor with high stability and sensitivity based on silver nanowire–graphene–tio(2) for the detection of organophosphate pesticides
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9069864/
https://www.ncbi.nlm.nih.gov/pubmed/35528694
http://dx.doi.org/10.1039/c9ra02140j
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