Medium optimization based on comparative metabolomic analysis of chicken embryo fibroblast DF-1 cells

Chicken embryo fibroblast DF-1 cells are increasingly being used in the production of avian virus vaccines. However, the relatively low proliferative capacity does not meet the requirements of industrial production. In this study, we attempted to improve the proliferative capacity of DF-1 cells. The results of intracellular metabolomics showed that 28 types of metabolites could play roles in DF-1 cell growth based on the variance and timing analysis of intracellular metabolites from DF-1 cells grown in two media with distinct growth difference, DMEM/F12 (1 : 1) and DMEM. By examining the differences in the components in the two media, DOE was used to screen and optimize the growth medium for DF-1 cells. The maximum cell density was 40.72% higher, and the infectious bursal disease virus (IBDV) titer was 2.68 times higher, in the optimized medium than in the control. This study proposes a complete solution from metabolomics to media optimization.