Cargando…

Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline

X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implem...

Descripción completa

Detalles Bibliográficos
Autores principales: Sala, Simone, Zhang, Yuhe, De La Rosa, Nathaly, Dreier, Till, Kahnt, Maik, Langer, Max, Dahlin, Lars B., Bech, Martin, Villanueva-Perez, Pablo, Kalbfleisch, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070709/
https://www.ncbi.nlm.nih.gov/pubmed/35511013
http://dx.doi.org/10.1107/S1600577522001874
Descripción
Sumario:X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view.