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Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline

X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implem...

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Autores principales: Sala, Simone, Zhang, Yuhe, De La Rosa, Nathaly, Dreier, Till, Kahnt, Maik, Langer, Max, Dahlin, Lars B., Bech, Martin, Villanueva-Perez, Pablo, Kalbfleisch, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070709/
https://www.ncbi.nlm.nih.gov/pubmed/35511013
http://dx.doi.org/10.1107/S1600577522001874
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author Sala, Simone
Zhang, Yuhe
De La Rosa, Nathaly
Dreier, Till
Kahnt, Maik
Langer, Max
Dahlin, Lars B.
Bech, Martin
Villanueva-Perez, Pablo
Kalbfleisch, Sebastian
author_facet Sala, Simone
Zhang, Yuhe
De La Rosa, Nathaly
Dreier, Till
Kahnt, Maik
Langer, Max
Dahlin, Lars B.
Bech, Martin
Villanueva-Perez, Pablo
Kalbfleisch, Sebastian
author_sort Sala, Simone
collection PubMed
description X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view.
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spelling pubmed-90707092022-05-10 Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline Sala, Simone Zhang, Yuhe De La Rosa, Nathaly Dreier, Till Kahnt, Maik Langer, Max Dahlin, Lars B. Bech, Martin Villanueva-Perez, Pablo Kalbfleisch, Sebastian J Synchrotron Radiat Research Papers X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view. International Union of Crystallography 2022-03-16 /pmc/articles/PMC9070709/ /pubmed/35511013 http://dx.doi.org/10.1107/S1600577522001874 Text en © Simone Sala et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Papers
Sala, Simone
Zhang, Yuhe
De La Rosa, Nathaly
Dreier, Till
Kahnt, Maik
Langer, Max
Dahlin, Lars B.
Bech, Martin
Villanueva-Perez, Pablo
Kalbfleisch, Sebastian
Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline
title Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline
title_full Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline
title_fullStr Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline
title_full_unstemmed Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline
title_short Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline
title_sort dose-efficient multimodal microscopy of human tissue at a hard x-ray nanoprobe beamline
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070709/
https://www.ncbi.nlm.nih.gov/pubmed/35511013
http://dx.doi.org/10.1107/S1600577522001874
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