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Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline
X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implem...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
International Union of Crystallography
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070709/ https://www.ncbi.nlm.nih.gov/pubmed/35511013 http://dx.doi.org/10.1107/S1600577522001874 |
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author | Sala, Simone Zhang, Yuhe De La Rosa, Nathaly Dreier, Till Kahnt, Maik Langer, Max Dahlin, Lars B. Bech, Martin Villanueva-Perez, Pablo Kalbfleisch, Sebastian |
author_facet | Sala, Simone Zhang, Yuhe De La Rosa, Nathaly Dreier, Till Kahnt, Maik Langer, Max Dahlin, Lars B. Bech, Martin Villanueva-Perez, Pablo Kalbfleisch, Sebastian |
author_sort | Sala, Simone |
collection | PubMed |
description | X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view. |
format | Online Article Text |
id | pubmed-9070709 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | International Union of Crystallography |
record_format | MEDLINE/PubMed |
spelling | pubmed-90707092022-05-10 Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline Sala, Simone Zhang, Yuhe De La Rosa, Nathaly Dreier, Till Kahnt, Maik Langer, Max Dahlin, Lars B. Bech, Martin Villanueva-Perez, Pablo Kalbfleisch, Sebastian J Synchrotron Radiat Research Papers X-ray fluorescence microscopy performed at nanofocusing synchrotron beamlines produces quantitative elemental distribution maps at unprecedented resolution (down to a few tens of nanometres), at the expense of relatively long measuring times and high absorbed doses. In this work, a method was implemented in which fast low-dose in-line holography was used to produce quantitative electron density maps at the mesoscale prior to nanoscale X-ray fluorescence acquisition. These maps ensure more efficient fluorescence scans and the reduction of the total absorbed dose, often relevant for radiation-sensitive (e.g. biological) samples. This multimodal microscopy approach was demonstrated on human sural nerve tissue. The two imaging modes provide complementary information at a comparable resolution, ultimately limited by the focal spot size. The experimental setup presented allows the user to swap between them in a flexible and reproducible fashion, as well as to easily adapt the scanning parameters during an experiment to fine-tune resolution and field of view. International Union of Crystallography 2022-03-16 /pmc/articles/PMC9070709/ /pubmed/35511013 http://dx.doi.org/10.1107/S1600577522001874 Text en © Simone Sala et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
spellingShingle | Research Papers Sala, Simone Zhang, Yuhe De La Rosa, Nathaly Dreier, Till Kahnt, Maik Langer, Max Dahlin, Lars B. Bech, Martin Villanueva-Perez, Pablo Kalbfleisch, Sebastian Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline |
title | Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline |
title_full | Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline |
title_fullStr | Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline |
title_full_unstemmed | Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline |
title_short | Dose-efficient multimodal microscopy of human tissue at a hard X-ray nanoprobe beamline |
title_sort | dose-efficient multimodal microscopy of human tissue at a hard x-ray nanoprobe beamline |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070709/ https://www.ncbi.nlm.nih.gov/pubmed/35511013 http://dx.doi.org/10.1107/S1600577522001874 |
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