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Excretion and viability of SARS-CoV-2 in feces and its association with the clinical outcome of COVID-19
The main objective was to evaluate the viability of the SARS-CoV-2 viral particles excreted in stools. In addition, we aimed to identify clinical factors associated with the detection of SARS-CoV-2 RNA in feces, and to determine if its presence is associated with an unfavorable clinical outcome, def...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070969/ https://www.ncbi.nlm.nih.gov/pubmed/35513481 http://dx.doi.org/10.1038/s41598-022-11439-7 |
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author | Cerrada-Romero, Cristina Berastegui-Cabrera, Judith Camacho-Martínez, Pedro Goikoetxea-Aguirre, Josune Pérez-Palacios, Patricia Santibáñez, Sonia José Blanco-Vidal, María Valiente, Adoración Alba, Jorge Rodríguez-Álvarez, Regino Pascual, Álvaro Oteo, José Antonio Miguel Cisneros, José Pachón, Jerónimo Casas-Flecha, Inmaculada Cordero, Elisa Pozo, Francisco Sánchez-Céspedes, Javier |
author_facet | Cerrada-Romero, Cristina Berastegui-Cabrera, Judith Camacho-Martínez, Pedro Goikoetxea-Aguirre, Josune Pérez-Palacios, Patricia Santibáñez, Sonia José Blanco-Vidal, María Valiente, Adoración Alba, Jorge Rodríguez-Álvarez, Regino Pascual, Álvaro Oteo, José Antonio Miguel Cisneros, José Pachón, Jerónimo Casas-Flecha, Inmaculada Cordero, Elisa Pozo, Francisco Sánchez-Céspedes, Javier |
author_sort | Cerrada-Romero, Cristina |
collection | PubMed |
description | The main objective was to evaluate the viability of the SARS-CoV-2 viral particles excreted in stools. In addition, we aimed to identify clinical factors associated with the detection of SARS-CoV-2 RNA in feces, and to determine if its presence is associated with an unfavorable clinical outcome, defined as intensive care unit (ICU) admission and/or death. A prospective multicenter cohort study of COVID-19 adult patients, with confirmed SARS-CoV-2 infection by RT-PCR assay in nasopharyngeal (NP) swabs admitted to four hospitals in Spain, from March 2020 to February 2021. Sixty-two adult COVID-19 patients had stool samples collected at admission and/or during the follow up, with a total of 79 stool samples. SARS-CoV-2 RNA was detected in stool samples from 27 (43.5%) out of the 62 patients. Replicative virus, measured by the generation of cytopathic effect in cell culture and subsequent RT-PCR confirmation of a decrease in the Ct values, was not found in any of these stool samples. Fecal virus excretion was not associated with the presence of gastrointestinal symptoms, or with differences in the evolution of COVID-19 patients. Our results suggest that SARS-CoV-2 replicative capacity is null or very limited in stool samples, and thus, the fecal–oral transmission of SARS-CoV-2 as an alternative infection route is highly unlikely. In our study, the detection of SARS-CoV-2 RNA in feces at the beginning of the disease is not associated with any clinical factor nor with an unfavorable clinical outcome. |
format | Online Article Text |
id | pubmed-9070969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90709692022-05-06 Excretion and viability of SARS-CoV-2 in feces and its association with the clinical outcome of COVID-19 Cerrada-Romero, Cristina Berastegui-Cabrera, Judith Camacho-Martínez, Pedro Goikoetxea-Aguirre, Josune Pérez-Palacios, Patricia Santibáñez, Sonia José Blanco-Vidal, María Valiente, Adoración Alba, Jorge Rodríguez-Álvarez, Regino Pascual, Álvaro Oteo, José Antonio Miguel Cisneros, José Pachón, Jerónimo Casas-Flecha, Inmaculada Cordero, Elisa Pozo, Francisco Sánchez-Céspedes, Javier Sci Rep Article The main objective was to evaluate the viability of the SARS-CoV-2 viral particles excreted in stools. In addition, we aimed to identify clinical factors associated with the detection of SARS-CoV-2 RNA in feces, and to determine if its presence is associated with an unfavorable clinical outcome, defined as intensive care unit (ICU) admission and/or death. A prospective multicenter cohort study of COVID-19 adult patients, with confirmed SARS-CoV-2 infection by RT-PCR assay in nasopharyngeal (NP) swabs admitted to four hospitals in Spain, from March 2020 to February 2021. Sixty-two adult COVID-19 patients had stool samples collected at admission and/or during the follow up, with a total of 79 stool samples. SARS-CoV-2 RNA was detected in stool samples from 27 (43.5%) out of the 62 patients. Replicative virus, measured by the generation of cytopathic effect in cell culture and subsequent RT-PCR confirmation of a decrease in the Ct values, was not found in any of these stool samples. Fecal virus excretion was not associated with the presence of gastrointestinal symptoms, or with differences in the evolution of COVID-19 patients. Our results suggest that SARS-CoV-2 replicative capacity is null or very limited in stool samples, and thus, the fecal–oral transmission of SARS-CoV-2 as an alternative infection route is highly unlikely. In our study, the detection of SARS-CoV-2 RNA in feces at the beginning of the disease is not associated with any clinical factor nor with an unfavorable clinical outcome. Nature Publishing Group UK 2022-05-05 /pmc/articles/PMC9070969/ /pubmed/35513481 http://dx.doi.org/10.1038/s41598-022-11439-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Cerrada-Romero, Cristina Berastegui-Cabrera, Judith Camacho-Martínez, Pedro Goikoetxea-Aguirre, Josune Pérez-Palacios, Patricia Santibáñez, Sonia José Blanco-Vidal, María Valiente, Adoración Alba, Jorge Rodríguez-Álvarez, Regino Pascual, Álvaro Oteo, José Antonio Miguel Cisneros, José Pachón, Jerónimo Casas-Flecha, Inmaculada Cordero, Elisa Pozo, Francisco Sánchez-Céspedes, Javier Excretion and viability of SARS-CoV-2 in feces and its association with the clinical outcome of COVID-19 |
title | Excretion and viability of SARS-CoV-2 in feces and its association with the clinical outcome of COVID-19 |
title_full | Excretion and viability of SARS-CoV-2 in feces and its association with the clinical outcome of COVID-19 |
title_fullStr | Excretion and viability of SARS-CoV-2 in feces and its association with the clinical outcome of COVID-19 |
title_full_unstemmed | Excretion and viability of SARS-CoV-2 in feces and its association with the clinical outcome of COVID-19 |
title_short | Excretion and viability of SARS-CoV-2 in feces and its association with the clinical outcome of COVID-19 |
title_sort | excretion and viability of sars-cov-2 in feces and its association with the clinical outcome of covid-19 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9070969/ https://www.ncbi.nlm.nih.gov/pubmed/35513481 http://dx.doi.org/10.1038/s41598-022-11439-7 |
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