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Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme

We present a novel protocol for the synthesis of enzymatically active microgels. The protocol is based on the precipitation polymerization of N-isopropylacrylamide (NIPAm) in the presence of an enzyme and a protein binding comonomer. A basic investigation on the influence of different reaction param...

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Detalles Bibliográficos
Autores principales: Reinicke, Stefan, Fischer, Thilo, Bramski, Julia, Pietruszka, Jörg, Böker, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071056/
https://www.ncbi.nlm.nih.gov/pubmed/35529607
http://dx.doi.org/10.1039/c9ra04000e
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author Reinicke, Stefan
Fischer, Thilo
Bramski, Julia
Pietruszka, Jörg
Böker, Alexander
author_facet Reinicke, Stefan
Fischer, Thilo
Bramski, Julia
Pietruszka, Jörg
Böker, Alexander
author_sort Reinicke, Stefan
collection PubMed
description We present a novel protocol for the synthesis of enzymatically active microgels. The protocol is based on the precipitation polymerization of N-isopropylacrylamide (NIPAm) in the presence of an enzyme and a protein binding comonomer. A basic investigation on the influence of different reaction parameters such as monomer concentration and reaction temperature on the microgel size and size distribution is performed and immobilization yields are determined. Microgels exhibiting hydrodynamic diameters between 100 nm and 1 μm and narrow size distribution could be synthesized while about 31–44% of the enzyme present in the initial reaction mixture can be immobilized. Successful immobilization including a verification of enzymatic activity of the microgels is achieved for glucose oxidase (GOx) and 2-deoxy-d-ribose-5-phosphate aldolase (DERA). The thermoresponsive properties of the microgels are assessed and discussed in the light of activity evolution with temperature. The positive correlation of enzymatic activity with temperature for the GOx containing microgel originates from a direct interaction of the enzyme with the PNIPAm based polymer matrix whose magnitude is highly influenced by temperature.
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spelling pubmed-90710562022-05-06 Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme Reinicke, Stefan Fischer, Thilo Bramski, Julia Pietruszka, Jörg Böker, Alexander RSC Adv Chemistry We present a novel protocol for the synthesis of enzymatically active microgels. The protocol is based on the precipitation polymerization of N-isopropylacrylamide (NIPAm) in the presence of an enzyme and a protein binding comonomer. A basic investigation on the influence of different reaction parameters such as monomer concentration and reaction temperature on the microgel size and size distribution is performed and immobilization yields are determined. Microgels exhibiting hydrodynamic diameters between 100 nm and 1 μm and narrow size distribution could be synthesized while about 31–44% of the enzyme present in the initial reaction mixture can be immobilized. Successful immobilization including a verification of enzymatic activity of the microgels is achieved for glucose oxidase (GOx) and 2-deoxy-d-ribose-5-phosphate aldolase (DERA). The thermoresponsive properties of the microgels are assessed and discussed in the light of activity evolution with temperature. The positive correlation of enzymatic activity with temperature for the GOx containing microgel originates from a direct interaction of the enzyme with the PNIPAm based polymer matrix whose magnitude is highly influenced by temperature. The Royal Society of Chemistry 2019-09-09 /pmc/articles/PMC9071056/ /pubmed/35529607 http://dx.doi.org/10.1039/c9ra04000e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Reinicke, Stefan
Fischer, Thilo
Bramski, Julia
Pietruszka, Jörg
Böker, Alexander
Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme
title Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme
title_full Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme
title_fullStr Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme
title_full_unstemmed Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme
title_short Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme
title_sort biocatalytically active microgels by precipitation polymerization of n-isopropyl acrylamide in the presence of an enzyme
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071056/
https://www.ncbi.nlm.nih.gov/pubmed/35529607
http://dx.doi.org/10.1039/c9ra04000e
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