Cargando…
Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype
Lung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approac...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071164/ https://www.ncbi.nlm.nih.gov/pubmed/35512017 http://dx.doi.org/10.1371/journal.pone.0268073 |
_version_ | 1784700792543903744 |
---|---|
author | Ciereszko, Andrzej Dietrich, Mariola A. Słowińska, Mariola Nynca, Joanna Ciborowski, Michał Kaczmarek, Monika M. Myszczyński, Kamil Kiśluk, Joanna Majewska, Anna Michalska-Falkowska, Anna Kodzik, Natalia Reszeć, Joanna Sierko, Ewa Nikliński, Jacek |
author_facet | Ciereszko, Andrzej Dietrich, Mariola A. Słowińska, Mariola Nynca, Joanna Ciborowski, Michał Kaczmarek, Monika M. Myszczyński, Kamil Kiśluk, Joanna Majewska, Anna Michalska-Falkowska, Anna Kodzik, Natalia Reszeć, Joanna Sierko, Ewa Nikliński, Jacek |
author_sort | Ciereszko, Andrzej |
collection | PubMed |
description | Lung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approach, we sought to compare tissue samples from non-small-cell lung cancer (NSCLC) patients taken from the tumor center and tumor margin. Two subtypes of NSCLC, adenocarcinoma (ADC) and squamous cell carcinoma (SCC) were compared. Data are available via ProteomeXchange with identifier PXD032736 and PXD032962 for ADC and SCC, respectively. For ADC proteins, 26 significant canonical pathways were identified, including Rho signaling pathways, a semaphorin neuronal repulsive signaling pathway, and epithelial adherens junction signaling. For SCC proteins, nine significant canonical pathways were identified, including hypoxia-inducible factor-1α signaling, thyroid hormone biosynthesis, and phagosome maturation. Proteins differentiating the tumor center and tumor margin were linked to cancer invasion and progression, including cell migration, adhesion and invasion, cytoskeletal structure, protein folding, anaerobic metabolism, tumor angiogenesis, EMC transition, epithelial adherens junctions, and inflammatory responses. In conclusion, we identified several proteins that are important for the better characterization of tumor development and molecular specificity of both lung cancer subtypes. We also identified proteins that may be important as biomarkers and/or targets for anticancer therapy. |
format | Online Article Text |
id | pubmed-9071164 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-90711642022-05-06 Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype Ciereszko, Andrzej Dietrich, Mariola A. Słowińska, Mariola Nynca, Joanna Ciborowski, Michał Kaczmarek, Monika M. Myszczyński, Kamil Kiśluk, Joanna Majewska, Anna Michalska-Falkowska, Anna Kodzik, Natalia Reszeć, Joanna Sierko, Ewa Nikliński, Jacek PLoS One Research Article Lung cancer is responsible for the most cancer-related mortality worldwide and the mechanism of its development is poorly understood. Proteomics has become a powerful tool offering vital knowledge related to cancer development. Using a two-dimensional difference gel electrophoresis (2D-DIGE) approach, we sought to compare tissue samples from non-small-cell lung cancer (NSCLC) patients taken from the tumor center and tumor margin. Two subtypes of NSCLC, adenocarcinoma (ADC) and squamous cell carcinoma (SCC) were compared. Data are available via ProteomeXchange with identifier PXD032736 and PXD032962 for ADC and SCC, respectively. For ADC proteins, 26 significant canonical pathways were identified, including Rho signaling pathways, a semaphorin neuronal repulsive signaling pathway, and epithelial adherens junction signaling. For SCC proteins, nine significant canonical pathways were identified, including hypoxia-inducible factor-1α signaling, thyroid hormone biosynthesis, and phagosome maturation. Proteins differentiating the tumor center and tumor margin were linked to cancer invasion and progression, including cell migration, adhesion and invasion, cytoskeletal structure, protein folding, anaerobic metabolism, tumor angiogenesis, EMC transition, epithelial adherens junctions, and inflammatory responses. In conclusion, we identified several proteins that are important for the better characterization of tumor development and molecular specificity of both lung cancer subtypes. We also identified proteins that may be important as biomarkers and/or targets for anticancer therapy. Public Library of Science 2022-05-05 /pmc/articles/PMC9071164/ /pubmed/35512017 http://dx.doi.org/10.1371/journal.pone.0268073 Text en © 2022 Ciereszko et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Ciereszko, Andrzej Dietrich, Mariola A. Słowińska, Mariola Nynca, Joanna Ciborowski, Michał Kaczmarek, Monika M. Myszczyński, Kamil Kiśluk, Joanna Majewska, Anna Michalska-Falkowska, Anna Kodzik, Natalia Reszeć, Joanna Sierko, Ewa Nikliński, Jacek Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype |
title | Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype |
title_full | Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype |
title_fullStr | Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype |
title_full_unstemmed | Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype |
title_short | Application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype |
title_sort | application of two-dimensional difference gel electrophoresis to identify protein changes between center, margin, and adjacent non-tumor tissues obtained from non-small-cell lung cancer with adenocarcinoma or squamous cell carcinoma subtype |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071164/ https://www.ncbi.nlm.nih.gov/pubmed/35512017 http://dx.doi.org/10.1371/journal.pone.0268073 |
work_keys_str_mv | AT ciereszkoandrzej applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT dietrichmariolaa applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT słowinskamariola applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT nyncajoanna applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT ciborowskimichał applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT kaczmarekmonikam applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT myszczynskikamil applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT kislukjoanna applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT majewskaanna applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT michalskafalkowskaanna applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT kodziknatalia applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT reszecjoanna applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT sierkoewa applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype AT niklinskijacek applicationoftwodimensionaldifferencegelelectrophoresistoidentifyproteinchangesbetweencentermarginandadjacentnontumortissuesobtainedfromnonsmallcelllungcancerwithadenocarcinomaorsquamouscellcarcinomasubtype |