Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species

We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from p...

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Autores principales: Luiza-Batista, Camilla, Nardella, Flore, Thiberge, Sabine, Serra-Hassoun, Malika, Ferreira, Marcelo U, Scherf, Artur, Garcia, Sylvie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Major Articles and Brief Reports
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071310/
https://www.ncbi.nlm.nih.gov/pubmed/34453537
http://dx.doi.org/10.1093/infdis/jiab431
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author Luiza-Batista, Camilla
Nardella, Flore
Thiberge, Sabine
Serra-Hassoun, Malika
Ferreira, Marcelo U
Scherf, Artur
Garcia, Sylvie
author_facet Luiza-Batista, Camilla
Nardella, Flore
Thiberge, Sabine
Serra-Hassoun, Malika
Ferreira, Marcelo U
Scherf, Artur
Garcia, Sylvie
author_sort Luiza-Batista, Camilla
collection PubMed
description We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from patient isolates. ImageStream analysis of liver stage sporozoites using a combination of surface circumsporozoite protein (CSP), deoxyribonucleic acid, and 18S RNA labeling proved that the new Flow-FISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites.
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spelling pubmed-90713102022-05-06 Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species Luiza-Batista, Camilla Nardella, Flore Thiberge, Sabine Serra-Hassoun, Malika Ferreira, Marcelo U Scherf, Artur Garcia, Sylvie J Infect Dis Major Articles and Brief Reports We adapted the RNA FISH Stellaris method to specifically detect the expression of Plasmodium genes by flow cytometry and ImageStream (Flow-FISH). This new method accurately quantified the erythrocytic forms of (1) Plasmodium falciparum and Plasmodium vivax and (2) the sexual stages of P vivax from patient isolates. ImageStream analysis of liver stage sporozoites using a combination of surface circumsporozoite protein (CSP), deoxyribonucleic acid, and 18S RNA labeling proved that the new Flow-FISH is suitable for gene expression studies of transmission stages. This powerful multiparametric single-cell method offers a platform of choice for both applied and fundamental research on the biology of malaria parasites. Oxford University Press 2021-08-28 /pmc/articles/PMC9071310/ /pubmed/34453537 http://dx.doi.org/10.1093/infdis/jiab431 Text en © The Author(s) 2021. Published by Oxford University Press for the Infectious Diseases Society of America. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Major Articles and Brief Reports
Luiza-Batista, Camilla
Nardella, Flore
Thiberge, Sabine
Serra-Hassoun, Malika
Ferreira, Marcelo U
Scherf, Artur
Garcia, Sylvie
Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species
title Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species
title_full Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species
title_fullStr Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species
title_full_unstemmed Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species
title_short Flowcytometric and ImageStream RNA-FISH Gene Expression, Quantification and Phenotypic Characterization of Blood Stages and Sporozoites From Human Malaria Species
title_sort flowcytometric and imagestream rna-fish gene expression, quantification and phenotypic characterization of blood stages and sporozoites from human malaria species
topic Major Articles and Brief Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071310/
https://www.ncbi.nlm.nih.gov/pubmed/34453537
http://dx.doi.org/10.1093/infdis/jiab431
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