Programmable cleavage of linear double-stranded DNA by combined action of Argonaute CbAgo from Clostridium butyricum and nuclease deficient RecBC helicase from E. coli

Prokaryotic Argonautes (pAgos) use small nucleic acids as specificity guides to cleave single-stranded DNA at complementary sequences. DNA targeting function of pAgos creates attractive opportunities for DNA manipulations that require programmable DNA cleavage. Currently, the use of mesophilic pAgos...

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Detalles Bibliográficos
Autores principales: Vaiskunaite, Rita, Vainauskas, Jogirdas, Morris, Janna J L, Potapov, Vladimir, Bitinaite, Jurate
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071414/
https://www.ncbi.nlm.nih.gov/pubmed/35420131
http://dx.doi.org/10.1093/nar/gkac229

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