Development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels

New bench-stable reagents with two diazonium sites were designed and synthesized for protein crosslinking. Because of the faster diazonium-tyrosine coupling reaction, hydrogels from the crosslinking of tobacco mosaic virus and the reagent DDA-3 could be prepared within 1 min at room temperature. Fur...

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Detalles Bibliográficos
Autores principales: Ma, Dejun, Chen, Zhuoyue, Yi, Long, Xi, Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071817/
https://www.ncbi.nlm.nih.gov/pubmed/35528434
http://dx.doi.org/10.1039/c9ra05630k
Descripción
Sumario:New bench-stable reagents with two diazonium sites were designed and synthesized for protein crosslinking. Because of the faster diazonium-tyrosine coupling reaction, hydrogels from the crosslinking of tobacco mosaic virus and the reagent DDA-3 could be prepared within 1 min at room temperature. Furthermore, hydrogels with the introduction of disulfide bonds viaDDA-4 could be chemically degraded by dithiothreitol. Our results provided a facile approach for the direct construction of virus-based hydrogels.

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