Development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels

New bench-stable reagents with two diazonium sites were designed and synthesized for protein crosslinking. Because of the faster diazonium-tyrosine coupling reaction, hydrogels from the crosslinking of tobacco mosaic virus and the reagent DDA-3 could be prepared within 1 min at room temperature. Fur...

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Detalles Bibliográficos
Autores principales: Ma, Dejun, Chen, Zhuoyue, Yi, Long, Xi, Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071817/
https://www.ncbi.nlm.nih.gov/pubmed/35528434
http://dx.doi.org/10.1039/c9ra05630k
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author Ma, Dejun
Chen, Zhuoyue
Yi, Long
Xi, Zhen
author_facet Ma, Dejun
Chen, Zhuoyue
Yi, Long
Xi, Zhen
author_sort Ma, Dejun
collection PubMed
description New bench-stable reagents with two diazonium sites were designed and synthesized for protein crosslinking. Because of the faster diazonium-tyrosine coupling reaction, hydrogels from the crosslinking of tobacco mosaic virus and the reagent DDA-3 could be prepared within 1 min at room temperature. Furthermore, hydrogels with the introduction of disulfide bonds viaDDA-4 could be chemically degraded by dithiothreitol. Our results provided a facile approach for the direct construction of virus-based hydrogels.
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spelling pubmed-90718172022-05-06 Development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels Ma, Dejun Chen, Zhuoyue Yi, Long Xi, Zhen RSC Adv Chemistry New bench-stable reagents with two diazonium sites were designed and synthesized for protein crosslinking. Because of the faster diazonium-tyrosine coupling reaction, hydrogels from the crosslinking of tobacco mosaic virus and the reagent DDA-3 could be prepared within 1 min at room temperature. Furthermore, hydrogels with the introduction of disulfide bonds viaDDA-4 could be chemically degraded by dithiothreitol. Our results provided a facile approach for the direct construction of virus-based hydrogels. The Royal Society of Chemistry 2019-09-17 /pmc/articles/PMC9071817/ /pubmed/35528434 http://dx.doi.org/10.1039/c9ra05630k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Ma, Dejun
Chen, Zhuoyue
Yi, Long
Xi, Zhen
Development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels
title Development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels
title_full Development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels
title_fullStr Development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels
title_full_unstemmed Development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels
title_short Development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels
title_sort development of improved dual-diazonium reagents for faster crosslinking of tobacco mosaic virus to form hydrogels
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071817/
https://www.ncbi.nlm.nih.gov/pubmed/35528434
http://dx.doi.org/10.1039/c9ra05630k
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AT chenzhuoyue developmentofimproveddualdiazoniumreagentsforfastercrosslinkingoftobaccomosaicvirustoformhydrogels
AT yilong developmentofimproveddualdiazoniumreagentsforfastercrosslinkingoftobaccomosaicvirustoformhydrogels
AT xizhen developmentofimproveddualdiazoniumreagentsforfastercrosslinkingoftobaccomosaicvirustoformhydrogels

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