Cargando…
Microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis
Collagen is a promising material for corneal tissue engineering. The surface topography of collagen is critical for cornea reconstruction and regeneration. Herein, we fabricated collagen films with microgrooved surface to investigate the effect of collagen film topography on corneal cell migration a...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071845/ https://www.ncbi.nlm.nih.gov/pubmed/35528407 http://dx.doi.org/10.1039/c9ra04009a |
_version_ | 1784700919405871104 |
---|---|
author | Xiong, Sijia Gao, Huichang Qin, Lanfeng Jia, Yongguang Gao, Meng Ren, Li |
author_facet | Xiong, Sijia Gao, Huichang Qin, Lanfeng Jia, Yongguang Gao, Meng Ren, Li |
author_sort | Xiong, Sijia |
collection | PubMed |
description | Collagen is a promising material for corneal tissue engineering. The surface topography of collagen is critical for cornea reconstruction and regeneration. Herein, we fabricated collagen films with microgrooved surface to investigate the effect of collagen film topography on corneal cell migration and antifibrosis. We found that the patterned films with microscale grooves could greatly affect the orientation, proliferation, migration, and gene expression of rabbit corneal epithelial cells and keratocytes. Compared with the natural cornea, the optimized collagen films with microgroove pattern show similar swelling performance, optical clarity, and biodegradability, which could efficiently promote the epithelial cell migration, accelerate wound healing process, and inhibit the fibrosis of keratocytes. These results suggest that collagen films with microgroove pattern are promising in corneal tissue engineering. |
format | Online Article Text |
id | pubmed-9071845 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90718452022-05-06 Microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis Xiong, Sijia Gao, Huichang Qin, Lanfeng Jia, Yongguang Gao, Meng Ren, Li RSC Adv Chemistry Collagen is a promising material for corneal tissue engineering. The surface topography of collagen is critical for cornea reconstruction and regeneration. Herein, we fabricated collagen films with microgrooved surface to investigate the effect of collagen film topography on corneal cell migration and antifibrosis. We found that the patterned films with microscale grooves could greatly affect the orientation, proliferation, migration, and gene expression of rabbit corneal epithelial cells and keratocytes. Compared with the natural cornea, the optimized collagen films with microgroove pattern show similar swelling performance, optical clarity, and biodegradability, which could efficiently promote the epithelial cell migration, accelerate wound healing process, and inhibit the fibrosis of keratocytes. These results suggest that collagen films with microgroove pattern are promising in corneal tissue engineering. The Royal Society of Chemistry 2019-09-18 /pmc/articles/PMC9071845/ /pubmed/35528407 http://dx.doi.org/10.1039/c9ra04009a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Xiong, Sijia Gao, Huichang Qin, Lanfeng Jia, Yongguang Gao, Meng Ren, Li Microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis |
title | Microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis |
title_full | Microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis |
title_fullStr | Microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis |
title_full_unstemmed | Microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis |
title_short | Microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis |
title_sort | microgrooved collagen-based corneal scaffold for promoting collective cell migration and antifibrosis |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9071845/ https://www.ncbi.nlm.nih.gov/pubmed/35528407 http://dx.doi.org/10.1039/c9ra04009a |
work_keys_str_mv | AT xiongsijia microgroovedcollagenbasedcornealscaffoldforpromotingcollectivecellmigrationandantifibrosis AT gaohuichang microgroovedcollagenbasedcornealscaffoldforpromotingcollectivecellmigrationandantifibrosis AT qinlanfeng microgroovedcollagenbasedcornealscaffoldforpromotingcollectivecellmigrationandantifibrosis AT jiayongguang microgroovedcollagenbasedcornealscaffoldforpromotingcollectivecellmigrationandantifibrosis AT gaomeng microgroovedcollagenbasedcornealscaffoldforpromotingcollectivecellmigrationandantifibrosis AT renli microgroovedcollagenbasedcornealscaffoldforpromotingcollectivecellmigrationandantifibrosis |