Cargando…

Effect of the Ethanol Extract of Red Okra Pods (Abelmoschus esculentus (L.) Moench) to Inhibit Cervical Cancer Cells Growth through Cell Cycle-Associated Oncogenes

This study aims to evaluate the potency of ethanol extract of red okra pods (EEROP) in inhibiting growth of cervical cancer cells through repression of the cell cycle-associated oncogenes. The EEROP treatment was given to HeLa cells cultured with RPMI medium and incubated at 37°C with 5% CO(2). The...

Descripción completa

Detalles Bibliográficos
Autores principales: Nisa, Nabilatun, Wahyuningsih, Sri Puji Astuti, Darmanto, Win, Purnama, Putut Rakhmad, Dewi, Firli Rahmah Primula, Soegiarti, Tipuk, Karsari, Deya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072054/
https://www.ncbi.nlm.nih.gov/pubmed/35529172
http://dx.doi.org/10.1155/2022/1094771
_version_ 1784700969694527488
author Nisa, Nabilatun
Wahyuningsih, Sri Puji Astuti
Darmanto, Win
Purnama, Putut Rakhmad
Dewi, Firli Rahmah Primula
Soegiarti, Tipuk
Karsari, Deya
author_facet Nisa, Nabilatun
Wahyuningsih, Sri Puji Astuti
Darmanto, Win
Purnama, Putut Rakhmad
Dewi, Firli Rahmah Primula
Soegiarti, Tipuk
Karsari, Deya
author_sort Nisa, Nabilatun
collection PubMed
description This study aims to evaluate the potency of ethanol extract of red okra pods (EEROP) in inhibiting growth of cervical cancer cells through repression of the cell cycle-associated oncogenes. The EEROP treatment was given to HeLa cells cultured with RPMI medium and incubated at 37°C with 5% CO(2). The MTT method was used to measure HeLa cell growth and IC(50) values. The mRNA levels of the three cell cycle-associated oncogenes (MYC, TYMS, and MDM2) were evaluated by qRT-PCR to determine the effect of EEROP treatment on the cell cycle. The lowest percentage of viable cells at 24, 48, and 72 hours after EEROP treatment was in the dose of 1000 μg/mL with a growth percentage of 71.60% at 24 hours, 55.61% at 48 hours, and 46.97% at 72 hours. The IC(50) values were 2845, 1153, and 776.8 μg/mL for 24, 48, and 72 hours, respectively. The three oncogenes at a dose of 1000 μg/mL significantly decreased the lowest mRNA levels compared to other doses with MYC oncogene that experienced the greatest decrease. The mRNA level of dose 1000 μg/mL EEROP at the MYC oncogene was 0.014-fold changes, at the TYMS oncogene was 0.097-fold changes, and at the MDM2 oncogene was 0.028-fold changes. The EEROP has been shown to decrease the expression of three cell cycle-associated oncogenes. This is also supported by the growth of HeLa cells that did not increase throughout 24, 48, and 72 hours. However, further research is needed on the main active components in red okra that function as anticancer, so that in the future, okra can not only stop cancer cell growth but also induce cancer cell death.
format Online
Article
Text
id pubmed-9072054
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-90720542022-05-06 Effect of the Ethanol Extract of Red Okra Pods (Abelmoschus esculentus (L.) Moench) to Inhibit Cervical Cancer Cells Growth through Cell Cycle-Associated Oncogenes Nisa, Nabilatun Wahyuningsih, Sri Puji Astuti Darmanto, Win Purnama, Putut Rakhmad Dewi, Firli Rahmah Primula Soegiarti, Tipuk Karsari, Deya Scientifica (Cairo) Research Article This study aims to evaluate the potency of ethanol extract of red okra pods (EEROP) in inhibiting growth of cervical cancer cells through repression of the cell cycle-associated oncogenes. The EEROP treatment was given to HeLa cells cultured with RPMI medium and incubated at 37°C with 5% CO(2). The MTT method was used to measure HeLa cell growth and IC(50) values. The mRNA levels of the three cell cycle-associated oncogenes (MYC, TYMS, and MDM2) were evaluated by qRT-PCR to determine the effect of EEROP treatment on the cell cycle. The lowest percentage of viable cells at 24, 48, and 72 hours after EEROP treatment was in the dose of 1000 μg/mL with a growth percentage of 71.60% at 24 hours, 55.61% at 48 hours, and 46.97% at 72 hours. The IC(50) values were 2845, 1153, and 776.8 μg/mL for 24, 48, and 72 hours, respectively. The three oncogenes at a dose of 1000 μg/mL significantly decreased the lowest mRNA levels compared to other doses with MYC oncogene that experienced the greatest decrease. The mRNA level of dose 1000 μg/mL EEROP at the MYC oncogene was 0.014-fold changes, at the TYMS oncogene was 0.097-fold changes, and at the MDM2 oncogene was 0.028-fold changes. The EEROP has been shown to decrease the expression of three cell cycle-associated oncogenes. This is also supported by the growth of HeLa cells that did not increase throughout 24, 48, and 72 hours. However, further research is needed on the main active components in red okra that function as anticancer, so that in the future, okra can not only stop cancer cell growth but also induce cancer cell death. Hindawi 2022-04-26 /pmc/articles/PMC9072054/ /pubmed/35529172 http://dx.doi.org/10.1155/2022/1094771 Text en Copyright © 2022 Nabilatun Nisa et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nisa, Nabilatun
Wahyuningsih, Sri Puji Astuti
Darmanto, Win
Purnama, Putut Rakhmad
Dewi, Firli Rahmah Primula
Soegiarti, Tipuk
Karsari, Deya
Effect of the Ethanol Extract of Red Okra Pods (Abelmoschus esculentus (L.) Moench) to Inhibit Cervical Cancer Cells Growth through Cell Cycle-Associated Oncogenes
title Effect of the Ethanol Extract of Red Okra Pods (Abelmoschus esculentus (L.) Moench) to Inhibit Cervical Cancer Cells Growth through Cell Cycle-Associated Oncogenes
title_full Effect of the Ethanol Extract of Red Okra Pods (Abelmoschus esculentus (L.) Moench) to Inhibit Cervical Cancer Cells Growth through Cell Cycle-Associated Oncogenes
title_fullStr Effect of the Ethanol Extract of Red Okra Pods (Abelmoschus esculentus (L.) Moench) to Inhibit Cervical Cancer Cells Growth through Cell Cycle-Associated Oncogenes
title_full_unstemmed Effect of the Ethanol Extract of Red Okra Pods (Abelmoschus esculentus (L.) Moench) to Inhibit Cervical Cancer Cells Growth through Cell Cycle-Associated Oncogenes
title_short Effect of the Ethanol Extract of Red Okra Pods (Abelmoschus esculentus (L.) Moench) to Inhibit Cervical Cancer Cells Growth through Cell Cycle-Associated Oncogenes
title_sort effect of the ethanol extract of red okra pods (abelmoschus esculentus (l.) moench) to inhibit cervical cancer cells growth through cell cycle-associated oncogenes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072054/
https://www.ncbi.nlm.nih.gov/pubmed/35529172
http://dx.doi.org/10.1155/2022/1094771
work_keys_str_mv AT nisanabilatun effectoftheethanolextractofredokrapodsabelmoschusesculentuslmoenchtoinhibitcervicalcancercellsgrowththroughcellcycleassociatedoncogenes
AT wahyuningsihsripujiastuti effectoftheethanolextractofredokrapodsabelmoschusesculentuslmoenchtoinhibitcervicalcancercellsgrowththroughcellcycleassociatedoncogenes
AT darmantowin effectoftheethanolextractofredokrapodsabelmoschusesculentuslmoenchtoinhibitcervicalcancercellsgrowththroughcellcycleassociatedoncogenes
AT purnamapututrakhmad effectoftheethanolextractofredokrapodsabelmoschusesculentuslmoenchtoinhibitcervicalcancercellsgrowththroughcellcycleassociatedoncogenes
AT dewifirlirahmahprimula effectoftheethanolextractofredokrapodsabelmoschusesculentuslmoenchtoinhibitcervicalcancercellsgrowththroughcellcycleassociatedoncogenes
AT soegiartitipuk effectoftheethanolextractofredokrapodsabelmoschusesculentuslmoenchtoinhibitcervicalcancercellsgrowththroughcellcycleassociatedoncogenes
AT karsarideya effectoftheethanolextractofredokrapodsabelmoschusesculentuslmoenchtoinhibitcervicalcancercellsgrowththroughcellcycleassociatedoncogenes