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Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method
Lipid distribution in intracellular vesicles is different from that in the plasma membrane of eukaryotic cells. The lipid components in the intracellular vesicles are composed of phosphatidylserine and phosphatidylethanolamine in the outer leaflet and phosphatidylcholine and sphingomyelin in the inn...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072116/ https://www.ncbi.nlm.nih.gov/pubmed/35530244 http://dx.doi.org/10.1039/c9ra04622d |
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author | Kamiya, Koki Osaki, Toshihisa Takeuchi, Shoji |
author_facet | Kamiya, Koki Osaki, Toshihisa Takeuchi, Shoji |
author_sort | Kamiya, Koki |
collection | PubMed |
description | Lipid distribution in intracellular vesicles is different from that in the plasma membrane of eukaryotic cells. The lipid components in the intracellular vesicles are composed of phosphatidylserine and phosphatidylethanolamine in the outer leaflet and phosphatidylcholine and sphingomyelin in the inner leaflet. The lipid asymmetricities both in the intracellular vesicle membrane and the plasma membrane contribute to synaptic transmission functions. In this study, we developed a cell-sized asymmetric lipid vesicle system containing small-sized asymmetric lipid vesicles (of diameter 200–1000 nm) (asymmetric vesicles-in-a-vesicle), emulating lipid components in the plasma membrane and intracellular vesicle membrane of eukaryotic cells, using microfluidic technology. We successfully constructed an artificial exocytosis system using the asymmetric vesicles-in-a-vesicle system. This asymmetric vesicles-in-a-vesicle system will be helpful in understanding the mechanisms of vesicle transport, such as neurotransmission and exocytosis. |
format | Online Article Text |
id | pubmed-9072116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90721162022-05-06 Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method Kamiya, Koki Osaki, Toshihisa Takeuchi, Shoji RSC Adv Chemistry Lipid distribution in intracellular vesicles is different from that in the plasma membrane of eukaryotic cells. The lipid components in the intracellular vesicles are composed of phosphatidylserine and phosphatidylethanolamine in the outer leaflet and phosphatidylcholine and sphingomyelin in the inner leaflet. The lipid asymmetricities both in the intracellular vesicle membrane and the plasma membrane contribute to synaptic transmission functions. In this study, we developed a cell-sized asymmetric lipid vesicle system containing small-sized asymmetric lipid vesicles (of diameter 200–1000 nm) (asymmetric vesicles-in-a-vesicle), emulating lipid components in the plasma membrane and intracellular vesicle membrane of eukaryotic cells, using microfluidic technology. We successfully constructed an artificial exocytosis system using the asymmetric vesicles-in-a-vesicle system. This asymmetric vesicles-in-a-vesicle system will be helpful in understanding the mechanisms of vesicle transport, such as neurotransmission and exocytosis. The Royal Society of Chemistry 2019-09-24 /pmc/articles/PMC9072116/ /pubmed/35530244 http://dx.doi.org/10.1039/c9ra04622d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Kamiya, Koki Osaki, Toshihisa Takeuchi, Shoji Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method |
title | Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method |
title_full | Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method |
title_fullStr | Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method |
title_full_unstemmed | Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method |
title_short | Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method |
title_sort | formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072116/ https://www.ncbi.nlm.nih.gov/pubmed/35530244 http://dx.doi.org/10.1039/c9ra04622d |
work_keys_str_mv | AT kamiyakoki formationofvesiclesinavesiclewithasymmetriclipidcomponentsusingapulsedjetflowmethod AT osakitoshihisa formationofvesiclesinavesiclewithasymmetriclipidcomponentsusingapulsedjetflowmethod AT takeuchishoji formationofvesiclesinavesiclewithasymmetriclipidcomponentsusingapulsedjetflowmethod |