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Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method

Lipid distribution in intracellular vesicles is different from that in the plasma membrane of eukaryotic cells. The lipid components in the intracellular vesicles are composed of phosphatidylserine and phosphatidylethanolamine in the outer leaflet and phosphatidylcholine and sphingomyelin in the inn...

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Detalles Bibliográficos
Autores principales: Kamiya, Koki, Osaki, Toshihisa, Takeuchi, Shoji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072116/
https://www.ncbi.nlm.nih.gov/pubmed/35530244
http://dx.doi.org/10.1039/c9ra04622d
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author Kamiya, Koki
Osaki, Toshihisa
Takeuchi, Shoji
author_facet Kamiya, Koki
Osaki, Toshihisa
Takeuchi, Shoji
author_sort Kamiya, Koki
collection PubMed
description Lipid distribution in intracellular vesicles is different from that in the plasma membrane of eukaryotic cells. The lipid components in the intracellular vesicles are composed of phosphatidylserine and phosphatidylethanolamine in the outer leaflet and phosphatidylcholine and sphingomyelin in the inner leaflet. The lipid asymmetricities both in the intracellular vesicle membrane and the plasma membrane contribute to synaptic transmission functions. In this study, we developed a cell-sized asymmetric lipid vesicle system containing small-sized asymmetric lipid vesicles (of diameter 200–1000 nm) (asymmetric vesicles-in-a-vesicle), emulating lipid components in the plasma membrane and intracellular vesicle membrane of eukaryotic cells, using microfluidic technology. We successfully constructed an artificial exocytosis system using the asymmetric vesicles-in-a-vesicle system. This asymmetric vesicles-in-a-vesicle system will be helpful in understanding the mechanisms of vesicle transport, such as neurotransmission and exocytosis.
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spelling pubmed-90721162022-05-06 Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method Kamiya, Koki Osaki, Toshihisa Takeuchi, Shoji RSC Adv Chemistry Lipid distribution in intracellular vesicles is different from that in the plasma membrane of eukaryotic cells. The lipid components in the intracellular vesicles are composed of phosphatidylserine and phosphatidylethanolamine in the outer leaflet and phosphatidylcholine and sphingomyelin in the inner leaflet. The lipid asymmetricities both in the intracellular vesicle membrane and the plasma membrane contribute to synaptic transmission functions. In this study, we developed a cell-sized asymmetric lipid vesicle system containing small-sized asymmetric lipid vesicles (of diameter 200–1000 nm) (asymmetric vesicles-in-a-vesicle), emulating lipid components in the plasma membrane and intracellular vesicle membrane of eukaryotic cells, using microfluidic technology. We successfully constructed an artificial exocytosis system using the asymmetric vesicles-in-a-vesicle system. This asymmetric vesicles-in-a-vesicle system will be helpful in understanding the mechanisms of vesicle transport, such as neurotransmission and exocytosis. The Royal Society of Chemistry 2019-09-24 /pmc/articles/PMC9072116/ /pubmed/35530244 http://dx.doi.org/10.1039/c9ra04622d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Kamiya, Koki
Osaki, Toshihisa
Takeuchi, Shoji
Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method
title Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method
title_full Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method
title_fullStr Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method
title_full_unstemmed Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method
title_short Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method
title_sort formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072116/
https://www.ncbi.nlm.nih.gov/pubmed/35530244
http://dx.doi.org/10.1039/c9ra04622d
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