Improved hydrogen production in the single-chamber microbial electrolysis cell with inhibition of methanogenesis under alkaline conditions

The aim of this study was to investigate hydrogen production enhanced by methanogenesis inhibition in the single-chamber microbial electrolysis cell (MEC) under alkaline conditions. With 50 mM bicarbonate buffer and 1 g L(−1) acetate, the MEC was tested at pH = 8.5, 9.5, 10.5, and 11.2, respectively, within 124 d operation. Effective methanogenesis inhibition in the MEC increased with pH from 8.5 to 11.2. At pH 11.2, Methanobacteriaceae reached the lowest absolute quantity (i.e., biomass and mcrA gene copy number of methanogens) within the microbial community in the cathodic biofilm among the pH values. Under the alkaline conditions, a hydrogen percentage of 85–90% and a methane percentage < 15% were achieved within 25 cycles (50 d) of operation. The maximum current density in the MEC reached 83.7 ± 1.5 A m(−3) with the average electrical recovery of 171 ± 18% and overall energy recovery of 72 ± 3%. The excellent performance of the MEC at pH = 11.2 was attributed to the low abundance of methanogens within the cathodic biofilm (2.23 ± 0.46 copy per cm(2)), low cathodic biomass (0.12 ± 0.01 mg protein per g), and low anode potential (−0.228 mV vs. saturated calomel electrode). Results from this study should be valuable to expand applications of the MEC with methanogenesis inhibition in alkaline wastewater treatment.