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Development of a fused imidazo[1,2-a]pyridine based fluorescent probe for Fe(3+) and Hg(2+) in aqueous media and HeLa cells
A new fluorescent sensor 5 based on a fused imidazopyridine scaffold has been designed and synthesized via cascade cyclization. The reaction features the formation of three different C–N bonds in sequence. Imidazopyridine based fluorescent probe 5 exhibits highly sensitive and selective fluorescent...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072145/ https://www.ncbi.nlm.nih.gov/pubmed/35531502 http://dx.doi.org/10.1039/c9ra04743c |
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author | Srivastava, Suman Thakur, Neha Singh, Ashutosh Shukla, Poonam Maikhuri, Vipin Kumar Garg, Neha Prasad, Ashok Pandey, Rampal |
author_facet | Srivastava, Suman Thakur, Neha Singh, Ashutosh Shukla, Poonam Maikhuri, Vipin Kumar Garg, Neha Prasad, Ashok Pandey, Rampal |
author_sort | Srivastava, Suman |
collection | PubMed |
description | A new fluorescent sensor 5 based on a fused imidazopyridine scaffold has been designed and synthesized via cascade cyclization. The reaction features the formation of three different C–N bonds in sequence. Imidazopyridine based fluorescent probe 5 exhibits highly sensitive and selective fluorescent sensing for Fe(3+)(‘turn-on’) and Hg(2+)(‘turn-off’). The excellent selectivity of imidazopyridine for Fe(3+)/Hg(2+) was not hampered in the presence of any of the competing cations. The limit of detection (LOD) of 5 toward Fe(3+) and Hg(2+) has been estimated to be 4.0 ppb and 1.0 ppb, respectively, with a good linear relationship (R(2) = 0.99). Notably, 5 selectively detects Fe(3+)/Hg(2+) through fluorescence enhancement signalling both in vitro and in HeLa cells. |
format | Online Article Text |
id | pubmed-9072145 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90721452022-05-06 Development of a fused imidazo[1,2-a]pyridine based fluorescent probe for Fe(3+) and Hg(2+) in aqueous media and HeLa cells Srivastava, Suman Thakur, Neha Singh, Ashutosh Shukla, Poonam Maikhuri, Vipin Kumar Garg, Neha Prasad, Ashok Pandey, Rampal RSC Adv Chemistry A new fluorescent sensor 5 based on a fused imidazopyridine scaffold has been designed and synthesized via cascade cyclization. The reaction features the formation of three different C–N bonds in sequence. Imidazopyridine based fluorescent probe 5 exhibits highly sensitive and selective fluorescent sensing for Fe(3+)(‘turn-on’) and Hg(2+)(‘turn-off’). The excellent selectivity of imidazopyridine for Fe(3+)/Hg(2+) was not hampered in the presence of any of the competing cations. The limit of detection (LOD) of 5 toward Fe(3+) and Hg(2+) has been estimated to be 4.0 ppb and 1.0 ppb, respectively, with a good linear relationship (R(2) = 0.99). Notably, 5 selectively detects Fe(3+)/Hg(2+) through fluorescence enhancement signalling both in vitro and in HeLa cells. The Royal Society of Chemistry 2019-09-20 /pmc/articles/PMC9072145/ /pubmed/35531502 http://dx.doi.org/10.1039/c9ra04743c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Srivastava, Suman Thakur, Neha Singh, Ashutosh Shukla, Poonam Maikhuri, Vipin Kumar Garg, Neha Prasad, Ashok Pandey, Rampal Development of a fused imidazo[1,2-a]pyridine based fluorescent probe for Fe(3+) and Hg(2+) in aqueous media and HeLa cells |
title | Development of a fused imidazo[1,2-a]pyridine based fluorescent probe for Fe(3+) and Hg(2+) in aqueous media and HeLa cells |
title_full | Development of a fused imidazo[1,2-a]pyridine based fluorescent probe for Fe(3+) and Hg(2+) in aqueous media and HeLa cells |
title_fullStr | Development of a fused imidazo[1,2-a]pyridine based fluorescent probe for Fe(3+) and Hg(2+) in aqueous media and HeLa cells |
title_full_unstemmed | Development of a fused imidazo[1,2-a]pyridine based fluorescent probe for Fe(3+) and Hg(2+) in aqueous media and HeLa cells |
title_short | Development of a fused imidazo[1,2-a]pyridine based fluorescent probe for Fe(3+) and Hg(2+) in aqueous media and HeLa cells |
title_sort | development of a fused imidazo[1,2-a]pyridine based fluorescent probe for fe(3+) and hg(2+) in aqueous media and hela cells |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072145/ https://www.ncbi.nlm.nih.gov/pubmed/35531502 http://dx.doi.org/10.1039/c9ra04743c |
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