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Microwave assisted antibacterial action of Garcinia nanoparticles on Gram-negative bacteria

Owing to the existence of the outer membrane barrier, most antibacterial agents cannot penetrate Gram-negative bacteria and are ineffective. Here, we report a general method for narrow-spectrum antibacterial Garcinia nanoparticles that can only be effective to kill Gram-positive bacteria, to effecti...

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Detalles Bibliográficos
Autores principales: Qiao, Yuqian, Xu, Yingde, Liu, Xiangmei, Zheng, Yufeng, Li, Bo, Han, Yong, Li, Zhaoyang, Yeung, Kelvin Wai Kwok, Liang, Yanqin, Zhu, Shengli, Cui, Zhenduo, Wu, Shuilin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072325/
https://www.ncbi.nlm.nih.gov/pubmed/35513402
http://dx.doi.org/10.1038/s41467-022-30125-w
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author Qiao, Yuqian
Xu, Yingde
Liu, Xiangmei
Zheng, Yufeng
Li, Bo
Han, Yong
Li, Zhaoyang
Yeung, Kelvin Wai Kwok
Liang, Yanqin
Zhu, Shengli
Cui, Zhenduo
Wu, Shuilin
author_facet Qiao, Yuqian
Xu, Yingde
Liu, Xiangmei
Zheng, Yufeng
Li, Bo
Han, Yong
Li, Zhaoyang
Yeung, Kelvin Wai Kwok
Liang, Yanqin
Zhu, Shengli
Cui, Zhenduo
Wu, Shuilin
author_sort Qiao, Yuqian
collection PubMed
description Owing to the existence of the outer membrane barrier, most antibacterial agents cannot penetrate Gram-negative bacteria and are ineffective. Here, we report a general method for narrow-spectrum antibacterial Garcinia nanoparticles that can only be effective to kill Gram-positive bacteria, to effectively eliminate Gram-negative bacteria by creating transient nanopores in bacterial outer membrane to induce drug entry under microwaves assistance. In vitro, under 15 min of microwaves irradiation, the antibacterial efficiency of Garcinia nanoparticles against Escherichia coli can be enhanced from 6.73% to 99.48%. In vivo, MV-assisted GNs can effectively cure mice with bacterial pneumonia. The combination of molecular dynamics simulation and experimental results reveal that the robust anti-E. coli effectiveness of Garcinia nanoparticles is attributed to the synergy of Garcinia nanoparticles and microwaves. This work presents a strategy for effectively treating both Gram-negative and Gram-positive bacteria co-infected pneumonia using herbal medicine nanoparticles with MV assistance as an exogenous antibacterial auxiliary.
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spelling pubmed-90723252022-05-07 Microwave assisted antibacterial action of Garcinia nanoparticles on Gram-negative bacteria Qiao, Yuqian Xu, Yingde Liu, Xiangmei Zheng, Yufeng Li, Bo Han, Yong Li, Zhaoyang Yeung, Kelvin Wai Kwok Liang, Yanqin Zhu, Shengli Cui, Zhenduo Wu, Shuilin Nat Commun Article Owing to the existence of the outer membrane barrier, most antibacterial agents cannot penetrate Gram-negative bacteria and are ineffective. Here, we report a general method for narrow-spectrum antibacterial Garcinia nanoparticles that can only be effective to kill Gram-positive bacteria, to effectively eliminate Gram-negative bacteria by creating transient nanopores in bacterial outer membrane to induce drug entry under microwaves assistance. In vitro, under 15 min of microwaves irradiation, the antibacterial efficiency of Garcinia nanoparticles against Escherichia coli can be enhanced from 6.73% to 99.48%. In vivo, MV-assisted GNs can effectively cure mice with bacterial pneumonia. The combination of molecular dynamics simulation and experimental results reveal that the robust anti-E. coli effectiveness of Garcinia nanoparticles is attributed to the synergy of Garcinia nanoparticles and microwaves. This work presents a strategy for effectively treating both Gram-negative and Gram-positive bacteria co-infected pneumonia using herbal medicine nanoparticles with MV assistance as an exogenous antibacterial auxiliary. Nature Publishing Group UK 2022-05-05 /pmc/articles/PMC9072325/ /pubmed/35513402 http://dx.doi.org/10.1038/s41467-022-30125-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Qiao, Yuqian
Xu, Yingde
Liu, Xiangmei
Zheng, Yufeng
Li, Bo
Han, Yong
Li, Zhaoyang
Yeung, Kelvin Wai Kwok
Liang, Yanqin
Zhu, Shengli
Cui, Zhenduo
Wu, Shuilin
Microwave assisted antibacterial action of Garcinia nanoparticles on Gram-negative bacteria
title Microwave assisted antibacterial action of Garcinia nanoparticles on Gram-negative bacteria
title_full Microwave assisted antibacterial action of Garcinia nanoparticles on Gram-negative bacteria
title_fullStr Microwave assisted antibacterial action of Garcinia nanoparticles on Gram-negative bacteria
title_full_unstemmed Microwave assisted antibacterial action of Garcinia nanoparticles on Gram-negative bacteria
title_short Microwave assisted antibacterial action of Garcinia nanoparticles on Gram-negative bacteria
title_sort microwave assisted antibacterial action of garcinia nanoparticles on gram-negative bacteria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072325/
https://www.ncbi.nlm.nih.gov/pubmed/35513402
http://dx.doi.org/10.1038/s41467-022-30125-w
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