Cargando…

Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice

Atopic dermatitis (AD) is a common inflammatory skin disease, and its pathogenesis is closely associated with microbial homeostasis in the gut, namely the gut-skin axis. Particularly, recent metagenomics studies revealed that the abundance of two major bacterial species in the gut, Faecalibacterium...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Yoonmi, Byeon, Hye Rim, Jang, Seo-Yul, Hong, Moon-Gi, Kim, Dohak, Lee, Dokyung, Shin, Joo-Hyun, Kim, Yesol, Kang, Seung-Goo, Seo, Jae-Gu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072367/
https://www.ncbi.nlm.nih.gov/pubmed/35513696
http://dx.doi.org/10.1038/s41598-022-11048-4
_version_ 1784701045158445056
author Lee, Yoonmi
Byeon, Hye Rim
Jang, Seo-Yul
Hong, Moon-Gi
Kim, Dohak
Lee, Dokyung
Shin, Joo-Hyun
Kim, Yesol
Kang, Seung-Goo
Seo, Jae-Gu
author_facet Lee, Yoonmi
Byeon, Hye Rim
Jang, Seo-Yul
Hong, Moon-Gi
Kim, Dohak
Lee, Dokyung
Shin, Joo-Hyun
Kim, Yesol
Kang, Seung-Goo
Seo, Jae-Gu
author_sort Lee, Yoonmi
collection PubMed
description Atopic dermatitis (AD) is a common inflammatory skin disease, and its pathogenesis is closely associated with microbial homeostasis in the gut, namely the gut-skin axis. Particularly, recent metagenomics studies revealed that the abundance of two major bacterial species in the gut, Faecalibacterium prausnitzii and Akkermansia muciniphila, may play a critical role in the pathogenesis of AD, but the effect of these species in AD has not yet been elucidated. To evaluate the potential beneficial effect of F. prausnitzii or A. muciniphila in AD, we conducted an animal model study where F. prausnitzii EB-FPDK11 or A. muciniphila EB-AMDK19, isolated from humans, was orally administered to 2,5-dinitrochlorobenzene (DNCB)-induced AD models using NC/Nga mice at a daily dose of 10(8) CFUs/mouse for six weeks. As a result, the administration of each strain of F. prausnitzii and A. muciniphila improved AD-related markers, such as dermatitis score, scratching behavior, and serum immunoglobulin E level. Also, the F. prausnitzii and A. muciniphila treatments decreased the level of thymic stromal lymphopoietin (TSLP), triggering the production of T helper (Th) 2 cytokines, and improved the imbalance between the Th1 and Th2 immune responses induced by DNCB. Meanwhile, the oral administration of the bacteria enhanced the production of filaggrin in the skin and ZO-1 in the gut barrier, leading to the recovery of functions. Taken together, our findings suggest that F. prausnitzii EB-FPDK11 and A. muciniphila EB-AMDK19 have a therapeutic potential in AD, which should be verified in humans.
format Online
Article
Text
id pubmed-9072367
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-90723672022-05-07 Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice Lee, Yoonmi Byeon, Hye Rim Jang, Seo-Yul Hong, Moon-Gi Kim, Dohak Lee, Dokyung Shin, Joo-Hyun Kim, Yesol Kang, Seung-Goo Seo, Jae-Gu Sci Rep Article Atopic dermatitis (AD) is a common inflammatory skin disease, and its pathogenesis is closely associated with microbial homeostasis in the gut, namely the gut-skin axis. Particularly, recent metagenomics studies revealed that the abundance of two major bacterial species in the gut, Faecalibacterium prausnitzii and Akkermansia muciniphila, may play a critical role in the pathogenesis of AD, but the effect of these species in AD has not yet been elucidated. To evaluate the potential beneficial effect of F. prausnitzii or A. muciniphila in AD, we conducted an animal model study where F. prausnitzii EB-FPDK11 or A. muciniphila EB-AMDK19, isolated from humans, was orally administered to 2,5-dinitrochlorobenzene (DNCB)-induced AD models using NC/Nga mice at a daily dose of 10(8) CFUs/mouse for six weeks. As a result, the administration of each strain of F. prausnitzii and A. muciniphila improved AD-related markers, such as dermatitis score, scratching behavior, and serum immunoglobulin E level. Also, the F. prausnitzii and A. muciniphila treatments decreased the level of thymic stromal lymphopoietin (TSLP), triggering the production of T helper (Th) 2 cytokines, and improved the imbalance between the Th1 and Th2 immune responses induced by DNCB. Meanwhile, the oral administration of the bacteria enhanced the production of filaggrin in the skin and ZO-1 in the gut barrier, leading to the recovery of functions. Taken together, our findings suggest that F. prausnitzii EB-FPDK11 and A. muciniphila EB-AMDK19 have a therapeutic potential in AD, which should be verified in humans. Nature Publishing Group UK 2022-05-05 /pmc/articles/PMC9072367/ /pubmed/35513696 http://dx.doi.org/10.1038/s41598-022-11048-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lee, Yoonmi
Byeon, Hye Rim
Jang, Seo-Yul
Hong, Moon-Gi
Kim, Dohak
Lee, Dokyung
Shin, Joo-Hyun
Kim, Yesol
Kang, Seung-Goo
Seo, Jae-Gu
Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice
title Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice
title_full Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice
title_fullStr Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice
title_full_unstemmed Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice
title_short Oral administration of Faecalibacterium prausnitzii and Akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in DNCB induced NC/Nga mice
title_sort oral administration of faecalibacterium prausnitzii and akkermansia muciniphila strains from humans improves atopic dermatitis symptoms in dncb induced nc/nga mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072367/
https://www.ncbi.nlm.nih.gov/pubmed/35513696
http://dx.doi.org/10.1038/s41598-022-11048-4
work_keys_str_mv AT leeyoonmi oraladministrationoffaecalibacteriumprausnitziiandakkermansiamuciniphilastrainsfromhumansimprovesatopicdermatitissymptomsindncbinducedncngamice
AT byeonhyerim oraladministrationoffaecalibacteriumprausnitziiandakkermansiamuciniphilastrainsfromhumansimprovesatopicdermatitissymptomsindncbinducedncngamice
AT jangseoyul oraladministrationoffaecalibacteriumprausnitziiandakkermansiamuciniphilastrainsfromhumansimprovesatopicdermatitissymptomsindncbinducedncngamice
AT hongmoongi oraladministrationoffaecalibacteriumprausnitziiandakkermansiamuciniphilastrainsfromhumansimprovesatopicdermatitissymptomsindncbinducedncngamice
AT kimdohak oraladministrationoffaecalibacteriumprausnitziiandakkermansiamuciniphilastrainsfromhumansimprovesatopicdermatitissymptomsindncbinducedncngamice
AT leedokyung oraladministrationoffaecalibacteriumprausnitziiandakkermansiamuciniphilastrainsfromhumansimprovesatopicdermatitissymptomsindncbinducedncngamice
AT shinjoohyun oraladministrationoffaecalibacteriumprausnitziiandakkermansiamuciniphilastrainsfromhumansimprovesatopicdermatitissymptomsindncbinducedncngamice
AT kimyesol oraladministrationoffaecalibacteriumprausnitziiandakkermansiamuciniphilastrainsfromhumansimprovesatopicdermatitissymptomsindncbinducedncngamice
AT kangseunggoo oraladministrationoffaecalibacteriumprausnitziiandakkermansiamuciniphilastrainsfromhumansimprovesatopicdermatitissymptomsindncbinducedncngamice
AT seojaegu oraladministrationoffaecalibacteriumprausnitziiandakkermansiamuciniphilastrainsfromhumansimprovesatopicdermatitissymptomsindncbinducedncngamice