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Exploration of molecular mechanism of intraspecific cross-incompatibility in sweetpotato by transcriptome and metabolome analysis
Cross-incompatibility, frequently happening in intraspecific varieties, has seriously restricted sweetpotato breeding. However, the mechanism of sweetpotato intraspecific cross-incompatibility (ICI) remains largely unexplored, especially for molecular mechanism. Treatment by inducible reagent develo...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Netherlands
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072463/ https://www.ncbi.nlm.nih.gov/pubmed/35338442 http://dx.doi.org/10.1007/s11103-022-01259-8 |
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author | Yang, Yiling Zhang, Xiongjian Zou, Hongda Chen, Jingyi Wang, Zhangying Luo, Zhongxia Yao, Zhufang Fang, Boping Huang, Lifei |
author_facet | Yang, Yiling Zhang, Xiongjian Zou, Hongda Chen, Jingyi Wang, Zhangying Luo, Zhongxia Yao, Zhufang Fang, Boping Huang, Lifei |
author_sort | Yang, Yiling |
collection | PubMed |
description | Cross-incompatibility, frequently happening in intraspecific varieties, has seriously restricted sweetpotato breeding. However, the mechanism of sweetpotato intraspecific cross-incompatibility (ICI) remains largely unexplored, especially for molecular mechanism. Treatment by inducible reagent developed by our lab provides a method to generate material for mechanism study, which could promote incompatible pollen germination and tube growth in the ICI group. Based on the differential phenotypes between treated and untreated samples, transcriptome and metabolome were employed to explore the molecular mechanism of sweetpotato ICI in this study, taking varieties ‘Guangshu 146’ and ‘Shangshu 19’, a typical incompatible combination, as materials. The results from transcriptome analysis showed oxidation–reduction, cell wall metabolism, plant–pathogen interaction, and plant hormone signal transduction were the essential pathways for sweetpotato ICI regulation. The differentially expressed genes (DEGs) enriched in these pathways were the important candidate genes to response ICI. Metabolome analysis showed that multiple differential metabolites (DMs) involved oxidation–reduction were identified. The most significant DM identified in comparison between compatible and incompatible samples was vitexin-2-O-glucoside, a flavonoid metabolite. Corresponding to it, cytochrome P450s were the most DEGs identified in oxidation–reduction, which were implicated in flavonoid biosynthesis. It further suggested oxidation–reduction play an important role in sweetpotato ICI regulation. To validate function of oxidation–reduction, reactive oxygen species (ROS) was detected in compatible and incompatible samples. The green fluorescence was observed in incompatible but not in compatible samples. It indicated ROS regulated by oxidation–reduction is important pathway to response sweetpotato ICI. The results in this study would provide valuable insights into molecular mechanisms for sweetpotato ICI. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11103-022-01259-8. |
format | Online Article Text |
id | pubmed-9072463 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-90724632022-05-07 Exploration of molecular mechanism of intraspecific cross-incompatibility in sweetpotato by transcriptome and metabolome analysis Yang, Yiling Zhang, Xiongjian Zou, Hongda Chen, Jingyi Wang, Zhangying Luo, Zhongxia Yao, Zhufang Fang, Boping Huang, Lifei Plant Mol Biol Article Cross-incompatibility, frequently happening in intraspecific varieties, has seriously restricted sweetpotato breeding. However, the mechanism of sweetpotato intraspecific cross-incompatibility (ICI) remains largely unexplored, especially for molecular mechanism. Treatment by inducible reagent developed by our lab provides a method to generate material for mechanism study, which could promote incompatible pollen germination and tube growth in the ICI group. Based on the differential phenotypes between treated and untreated samples, transcriptome and metabolome were employed to explore the molecular mechanism of sweetpotato ICI in this study, taking varieties ‘Guangshu 146’ and ‘Shangshu 19’, a typical incompatible combination, as materials. The results from transcriptome analysis showed oxidation–reduction, cell wall metabolism, plant–pathogen interaction, and plant hormone signal transduction were the essential pathways for sweetpotato ICI regulation. The differentially expressed genes (DEGs) enriched in these pathways were the important candidate genes to response ICI. Metabolome analysis showed that multiple differential metabolites (DMs) involved oxidation–reduction were identified. The most significant DM identified in comparison between compatible and incompatible samples was vitexin-2-O-glucoside, a flavonoid metabolite. Corresponding to it, cytochrome P450s were the most DEGs identified in oxidation–reduction, which were implicated in flavonoid biosynthesis. It further suggested oxidation–reduction play an important role in sweetpotato ICI regulation. To validate function of oxidation–reduction, reactive oxygen species (ROS) was detected in compatible and incompatible samples. The green fluorescence was observed in incompatible but not in compatible samples. It indicated ROS regulated by oxidation–reduction is important pathway to response sweetpotato ICI. The results in this study would provide valuable insights into molecular mechanisms for sweetpotato ICI. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11103-022-01259-8. Springer Netherlands 2022-03-25 2022 /pmc/articles/PMC9072463/ /pubmed/35338442 http://dx.doi.org/10.1007/s11103-022-01259-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yang, Yiling Zhang, Xiongjian Zou, Hongda Chen, Jingyi Wang, Zhangying Luo, Zhongxia Yao, Zhufang Fang, Boping Huang, Lifei Exploration of molecular mechanism of intraspecific cross-incompatibility in sweetpotato by transcriptome and metabolome analysis |
title | Exploration of molecular mechanism of intraspecific cross-incompatibility in sweetpotato by transcriptome and metabolome analysis |
title_full | Exploration of molecular mechanism of intraspecific cross-incompatibility in sweetpotato by transcriptome and metabolome analysis |
title_fullStr | Exploration of molecular mechanism of intraspecific cross-incompatibility in sweetpotato by transcriptome and metabolome analysis |
title_full_unstemmed | Exploration of molecular mechanism of intraspecific cross-incompatibility in sweetpotato by transcriptome and metabolome analysis |
title_short | Exploration of molecular mechanism of intraspecific cross-incompatibility in sweetpotato by transcriptome and metabolome analysis |
title_sort | exploration of molecular mechanism of intraspecific cross-incompatibility in sweetpotato by transcriptome and metabolome analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9072463/ https://www.ncbi.nlm.nih.gov/pubmed/35338442 http://dx.doi.org/10.1007/s11103-022-01259-8 |
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