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Isolation and characterization of a highly effective bacterium Bacillus cereus b-525k for hexavalent chromium detoxification

The chromate resistant Gram-positive Bacillus cereus strain b-525k was isolated from tannery effluents, demonstrating optimal propagation at 37 °C and pH 8. The minimum inhibitory concentration (MIC) test showed that B. cereus b-525k can tolerate up to 32 mM Cr(6+), and also exhibit the ability to r...

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Autores principales: Elahi, Amina, Rehman, Abdul, Zajif Hussain, Syed, Zulfiqar, Soumble, Shakoori, Abdul Rauf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073032/
https://www.ncbi.nlm.nih.gov/pubmed/35531181
http://dx.doi.org/10.1016/j.sjbs.2022.01.027
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author Elahi, Amina
Rehman, Abdul
Zajif Hussain, Syed
Zulfiqar, Soumble
Shakoori, Abdul Rauf
author_facet Elahi, Amina
Rehman, Abdul
Zajif Hussain, Syed
Zulfiqar, Soumble
Shakoori, Abdul Rauf
author_sort Elahi, Amina
collection PubMed
description The chromate resistant Gram-positive Bacillus cereus strain b-525k was isolated from tannery effluents, demonstrating optimal propagation at 37 °C and pH 8. The minimum inhibitory concentration (MIC) test showed that B. cereus b-525k can tolerate up to 32 mM Cr(6+), and also exhibit the ability to resist other toxic metal ions including Pb(2+) (23 mM), As(3+) (21 mM), Zn(2+) (17 mM), Cd(2+) (5 mM), Cu(2+) (2 mM), and Ni(2+) (3 mM) with the resistance order as Cr (6+) > Pb(2+) > As(3+) >Zn(2+) >Cd(2+) >Ni(2+) >Cu(2+). B. cereus b-525k showed maximum biosorption efficiency (q) of 51 mM Cr(6+)/g after 6 days. Chromate stress elicited pronounced production of antioxidant enzymes such as catalase (CAT) 191%, glutathione transferase (GST) 192%, superoxide dismutase (SOD) 161%, peroxidase (POX) 199%, and ascorbate peroxidase (APOX) (154%). Within B. cereus b-525k, the influence of Cr(6+) stress (2 mM) did stimulate rise in levels of GSH (907%) and non-protein thiols (541%) was measured as compared to the control (without any Cr(6+) stress) which markedly nullifies Cr(6+) generated oxidative stress. The pilot scale experiments utilizing original tannery effluent showed that B. cereus b-525k could remove 99% Cr(6+) in 6 days, thus, it could be a potential candidate to reclaim the chromate contaminated sites.
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spelling pubmed-90730322022-05-07 Isolation and characterization of a highly effective bacterium Bacillus cereus b-525k for hexavalent chromium detoxification Elahi, Amina Rehman, Abdul Zajif Hussain, Syed Zulfiqar, Soumble Shakoori, Abdul Rauf Saudi J Biol Sci Original Article The chromate resistant Gram-positive Bacillus cereus strain b-525k was isolated from tannery effluents, demonstrating optimal propagation at 37 °C and pH 8. The minimum inhibitory concentration (MIC) test showed that B. cereus b-525k can tolerate up to 32 mM Cr(6+), and also exhibit the ability to resist other toxic metal ions including Pb(2+) (23 mM), As(3+) (21 mM), Zn(2+) (17 mM), Cd(2+) (5 mM), Cu(2+) (2 mM), and Ni(2+) (3 mM) with the resistance order as Cr (6+) > Pb(2+) > As(3+) >Zn(2+) >Cd(2+) >Ni(2+) >Cu(2+). B. cereus b-525k showed maximum biosorption efficiency (q) of 51 mM Cr(6+)/g after 6 days. Chromate stress elicited pronounced production of antioxidant enzymes such as catalase (CAT) 191%, glutathione transferase (GST) 192%, superoxide dismutase (SOD) 161%, peroxidase (POX) 199%, and ascorbate peroxidase (APOX) (154%). Within B. cereus b-525k, the influence of Cr(6+) stress (2 mM) did stimulate rise in levels of GSH (907%) and non-protein thiols (541%) was measured as compared to the control (without any Cr(6+) stress) which markedly nullifies Cr(6+) generated oxidative stress. The pilot scale experiments utilizing original tannery effluent showed that B. cereus b-525k could remove 99% Cr(6+) in 6 days, thus, it could be a potential candidate to reclaim the chromate contaminated sites. Elsevier 2022-04 2022-01-13 /pmc/articles/PMC9073032/ /pubmed/35531181 http://dx.doi.org/10.1016/j.sjbs.2022.01.027 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Elahi, Amina
Rehman, Abdul
Zajif Hussain, Syed
Zulfiqar, Soumble
Shakoori, Abdul Rauf
Isolation and characterization of a highly effective bacterium Bacillus cereus b-525k for hexavalent chromium detoxification
title Isolation and characterization of a highly effective bacterium Bacillus cereus b-525k for hexavalent chromium detoxification
title_full Isolation and characterization of a highly effective bacterium Bacillus cereus b-525k for hexavalent chromium detoxification
title_fullStr Isolation and characterization of a highly effective bacterium Bacillus cereus b-525k for hexavalent chromium detoxification
title_full_unstemmed Isolation and characterization of a highly effective bacterium Bacillus cereus b-525k for hexavalent chromium detoxification
title_short Isolation and characterization of a highly effective bacterium Bacillus cereus b-525k for hexavalent chromium detoxification
title_sort isolation and characterization of a highly effective bacterium bacillus cereus b-525k for hexavalent chromium detoxification
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073032/
https://www.ncbi.nlm.nih.gov/pubmed/35531181
http://dx.doi.org/10.1016/j.sjbs.2022.01.027
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