Cargando…

Ecogenotoxicological studies for an early toxicity screening and monitoring in Epinephalus chlorostigma and Scamberomorus commerson

The study was planned to investigate DNA fragmentation in fish to screen aquatic toxicity and in Epinephalus chlorostigma and Scamberomorus commerson collected from Red sea near Jizan, Saudi Arabia from three locations “(Corniche North park: “16.92161, 42.54631; Jizan Port: 16.874, 42.54952” N and J...

Descripción completa

Detalles Bibliográficos
Autores principales: Mahboob, Shahid, Ahmed, Zubair, Farooq Khan, Muhammad, Saho, Changwei, Virik, Promy, Al-Mulhm, N., Baabbad, Almohannad A.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073060/
https://www.ncbi.nlm.nih.gov/pubmed/35531145
http://dx.doi.org/10.1016/j.sjbs.2021.12.064
_version_ 1784701201998151680
author Mahboob, Shahid
Ahmed, Zubair
Farooq Khan, Muhammad
Saho, Changwei
Virik, Promy
Al-Mulhm, N.
Baabbad, Almohannad A.A.
author_facet Mahboob, Shahid
Ahmed, Zubair
Farooq Khan, Muhammad
Saho, Changwei
Virik, Promy
Al-Mulhm, N.
Baabbad, Almohannad A.A.
author_sort Mahboob, Shahid
collection PubMed
description The study was planned to investigate DNA fragmentation in fish to screen aquatic toxicity and in Epinephalus chlorostigma and Scamberomorus commerson collected from Red sea near Jizan, Saudi Arabia from three locations “(Corniche North park: “16.92161, 42.54631; Jizan Port: 16.874, 42.54952” N and Jizan Economic City: 17.26589, 42.34738“ ”)“ were used as a case study for the application of comet assay. The study area of the Red Sea is polluted due to anthropogenic activities and the disposal of wastes from multiple sources. Comet and micronucleus assays were used to detect genotoxicity in these fish species harvested from three sites. The concentration of Pb, Cr, Zn, Mn, Cu, Cd, Sn, and Hg was higher in the water samples collected from the polluted site compared to the non-polluted site of the Red sea. Comet assay for S. commerson showed significant (p < 0.05) genetic damage about 44.33 ± 3.03% DNA in comet tail at site S1. It was subsequently reduced to 31.71 ± 3.52% and 22.11 ± 2.52% at sites S2 and S3. E. chlorostigma also showed significant DNA in comet tail as 17.34 ± 2.19%, 11.87 ± 3.01%, and 36.41 ± 3.98% at site S1-S3, respectively. Significant (p < 0.05) DNA damage was observed in the fishes procured from non-polluted locations and upstream locations. The micronucleus induction in E. chlorostigma was recorded as 23.20 ± 4.19 and 2.20 ± 0.58%, respectively, non-polluted and polluted sites. S. commerson exhibited significant differences between polluted and non-polluted sites (44.80 ± 3.73 and 8.20 ± 2.20‰) polluted and upstream (44.80 ± 3.73 and 20.60 ± 4.02‰), respectively. A significant difference was obtained between E. chlorostigma and S. commerson for nuclear abnormalities S. commerson showed higher frequencies for nuclear deformities than E. chlorostigma. S. commerson showed substantial micronucleus induction frequencies collected from an area of low pollution intensity (upstream). This study showed that E. clorostigma and S. commerson could be successfully used as a bioindicator to determine the health of the Red Sea through the most specific assays such as comet and micronucleus tests as an early warning and to devise the monitoring strategies to ensure a safe supply of fish for human consumption.
format Online
Article
Text
id pubmed-9073060
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-90730602022-05-07 Ecogenotoxicological studies for an early toxicity screening and monitoring in Epinephalus chlorostigma and Scamberomorus commerson Mahboob, Shahid Ahmed, Zubair Farooq Khan, Muhammad Saho, Changwei Virik, Promy Al-Mulhm, N. Baabbad, Almohannad A.A. Saudi J Biol Sci Original Article The study was planned to investigate DNA fragmentation in fish to screen aquatic toxicity and in Epinephalus chlorostigma and Scamberomorus commerson collected from Red sea near Jizan, Saudi Arabia from three locations “(Corniche North park: “16.92161, 42.54631; Jizan Port: 16.874, 42.54952” N and Jizan Economic City: 17.26589, 42.34738“ ”)“ were used as a case study for the application of comet assay. The study area of the Red Sea is polluted due to anthropogenic activities and the disposal of wastes from multiple sources. Comet and micronucleus assays were used to detect genotoxicity in these fish species harvested from three sites. The concentration of Pb, Cr, Zn, Mn, Cu, Cd, Sn, and Hg was higher in the water samples collected from the polluted site compared to the non-polluted site of the Red sea. Comet assay for S. commerson showed significant (p < 0.05) genetic damage about 44.33 ± 3.03% DNA in comet tail at site S1. It was subsequently reduced to 31.71 ± 3.52% and 22.11 ± 2.52% at sites S2 and S3. E. chlorostigma also showed significant DNA in comet tail as 17.34 ± 2.19%, 11.87 ± 3.01%, and 36.41 ± 3.98% at site S1-S3, respectively. Significant (p < 0.05) DNA damage was observed in the fishes procured from non-polluted locations and upstream locations. The micronucleus induction in E. chlorostigma was recorded as 23.20 ± 4.19 and 2.20 ± 0.58%, respectively, non-polluted and polluted sites. S. commerson exhibited significant differences between polluted and non-polluted sites (44.80 ± 3.73 and 8.20 ± 2.20‰) polluted and upstream (44.80 ± 3.73 and 20.60 ± 4.02‰), respectively. A significant difference was obtained between E. chlorostigma and S. commerson for nuclear abnormalities S. commerson showed higher frequencies for nuclear deformities than E. chlorostigma. S. commerson showed substantial micronucleus induction frequencies collected from an area of low pollution intensity (upstream). This study showed that E. clorostigma and S. commerson could be successfully used as a bioindicator to determine the health of the Red Sea through the most specific assays such as comet and micronucleus tests as an early warning and to devise the monitoring strategies to ensure a safe supply of fish for human consumption. Elsevier 2022-04 2022-01-04 /pmc/articles/PMC9073060/ /pubmed/35531145 http://dx.doi.org/10.1016/j.sjbs.2021.12.064 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
Mahboob, Shahid
Ahmed, Zubair
Farooq Khan, Muhammad
Saho, Changwei
Virik, Promy
Al-Mulhm, N.
Baabbad, Almohannad A.A.
Ecogenotoxicological studies for an early toxicity screening and monitoring in Epinephalus chlorostigma and Scamberomorus commerson
title Ecogenotoxicological studies for an early toxicity screening and monitoring in Epinephalus chlorostigma and Scamberomorus commerson
title_full Ecogenotoxicological studies for an early toxicity screening and monitoring in Epinephalus chlorostigma and Scamberomorus commerson
title_fullStr Ecogenotoxicological studies for an early toxicity screening and monitoring in Epinephalus chlorostigma and Scamberomorus commerson
title_full_unstemmed Ecogenotoxicological studies for an early toxicity screening and monitoring in Epinephalus chlorostigma and Scamberomorus commerson
title_short Ecogenotoxicological studies for an early toxicity screening and monitoring in Epinephalus chlorostigma and Scamberomorus commerson
title_sort ecogenotoxicological studies for an early toxicity screening and monitoring in epinephalus chlorostigma and scamberomorus commerson
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073060/
https://www.ncbi.nlm.nih.gov/pubmed/35531145
http://dx.doi.org/10.1016/j.sjbs.2021.12.064
work_keys_str_mv AT mahboobshahid ecogenotoxicologicalstudiesforanearlytoxicityscreeningandmonitoringinepinephaluschlorostigmaandscamberomoruscommerson
AT ahmedzubair ecogenotoxicologicalstudiesforanearlytoxicityscreeningandmonitoringinepinephaluschlorostigmaandscamberomoruscommerson
AT farooqkhanmuhammad ecogenotoxicologicalstudiesforanearlytoxicityscreeningandmonitoringinepinephaluschlorostigmaandscamberomoruscommerson
AT sahochangwei ecogenotoxicologicalstudiesforanearlytoxicityscreeningandmonitoringinepinephaluschlorostigmaandscamberomoruscommerson
AT virikpromy ecogenotoxicologicalstudiesforanearlytoxicityscreeningandmonitoringinepinephaluschlorostigmaandscamberomoruscommerson
AT almulhmn ecogenotoxicologicalstudiesforanearlytoxicityscreeningandmonitoringinepinephaluschlorostigmaandscamberomoruscommerson
AT baabbadalmohannadaa ecogenotoxicologicalstudiesforanearlytoxicityscreeningandmonitoringinepinephaluschlorostigmaandscamberomoruscommerson