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Early selective strategies for higher yielding bio-economic Indian ginseng based on genotypic study through metabolic and molecular markers

Applying biotechnological tools to the selection of higher-yielding bioeconomic crops is a promising and remarkable means of reducing the burden on production on a global scale. In the present study, 25 germplasms of Indian ginseng (Withania somnifera (L.) Dunal) were examined for their genetic dive...

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Autores principales: Chauhan, Surya, Mandliya, Trapti, Jain, Devendra, Joshi, Arunabh, Khatik, Champa Lal, Singh, Abhijeet, Upadhyay, Sudhir K., Jain, Rohit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073062/
https://www.ncbi.nlm.nih.gov/pubmed/35531148
http://dx.doi.org/10.1016/j.sjbs.2022.01.030
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author Chauhan, Surya
Mandliya, Trapti
Jain, Devendra
Joshi, Arunabh
Khatik, Champa Lal
Singh, Abhijeet
Upadhyay, Sudhir K.
Jain, Rohit
author_facet Chauhan, Surya
Mandliya, Trapti
Jain, Devendra
Joshi, Arunabh
Khatik, Champa Lal
Singh, Abhijeet
Upadhyay, Sudhir K.
Jain, Rohit
author_sort Chauhan, Surya
collection PubMed
description Applying biotechnological tools to the selection of higher-yielding bioeconomic crops is a promising and remarkable means of reducing the burden on production on a global scale. In the present study, 25 germplasms of Indian ginseng (Withania somnifera (L.) Dunal) were examined for their genetic diversity by using morphological, biochemical, and molecular markers for twenty plant growth traits. The properties of plant growth differed significantly in the maximum genotypes of Indian ginseng, the markers of randomly amplified polymorphic DNA (RAPD), and inter simple sequence repeat (ISSR) showed considerable diversity between the genotypes. The combined unweighted pair group technique with arithmetic mean (UPGMA) dendrogram of morphological, biochemical, and molecular markers grouped all 25 genotypes into two main clusters at 0.61 coefficient value. In addition to this, secondary metabolite profiling by high-performance liquid chromatography (HPLC), there were high variations for withanolide B (WL-B), withanoside-V (WS-V), wedelolactone (WDL), withanoside-IV (WS-IV), and withaferin A (WF-A) content between different genotypes. For the total alkaloid and withanolide concentration in the roots and leaves, high heritability with an increased genetic gain was observed, indicating that selection based on these traits could be an effective method in breeding programs. Furthermore, the path coefficient analysis showed a direct positive impact of the total root fiber, WL-B (leaves), WF-A (leaves), WS-IV (roots), WDL (roots), and the total alkaloid content on the dry root yield. High content of WDL, a high-quality bioactive withanolide, was also described for the first time in the genotype UWS23. These properties can further be exploited to improve the dry root yield in W. somnifera genotypes. The outcomes of the present study also provide an essential foundation for the selection of high-yielding bioeconomic varieties that could be utilized to improve Ashwagandha breeding programs.
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spelling pubmed-90730622022-05-07 Early selective strategies for higher yielding bio-economic Indian ginseng based on genotypic study through metabolic and molecular markers Chauhan, Surya Mandliya, Trapti Jain, Devendra Joshi, Arunabh Khatik, Champa Lal Singh, Abhijeet Upadhyay, Sudhir K. Jain, Rohit Saudi J Biol Sci Original Article Applying biotechnological tools to the selection of higher-yielding bioeconomic crops is a promising and remarkable means of reducing the burden on production on a global scale. In the present study, 25 germplasms of Indian ginseng (Withania somnifera (L.) Dunal) were examined for their genetic diversity by using morphological, biochemical, and molecular markers for twenty plant growth traits. The properties of plant growth differed significantly in the maximum genotypes of Indian ginseng, the markers of randomly amplified polymorphic DNA (RAPD), and inter simple sequence repeat (ISSR) showed considerable diversity between the genotypes. The combined unweighted pair group technique with arithmetic mean (UPGMA) dendrogram of morphological, biochemical, and molecular markers grouped all 25 genotypes into two main clusters at 0.61 coefficient value. In addition to this, secondary metabolite profiling by high-performance liquid chromatography (HPLC), there were high variations for withanolide B (WL-B), withanoside-V (WS-V), wedelolactone (WDL), withanoside-IV (WS-IV), and withaferin A (WF-A) content between different genotypes. For the total alkaloid and withanolide concentration in the roots and leaves, high heritability with an increased genetic gain was observed, indicating that selection based on these traits could be an effective method in breeding programs. Furthermore, the path coefficient analysis showed a direct positive impact of the total root fiber, WL-B (leaves), WF-A (leaves), WS-IV (roots), WDL (roots), and the total alkaloid content on the dry root yield. High content of WDL, a high-quality bioactive withanolide, was also described for the first time in the genotype UWS23. These properties can further be exploited to improve the dry root yield in W. somnifera genotypes. The outcomes of the present study also provide an essential foundation for the selection of high-yielding bioeconomic varieties that could be utilized to improve Ashwagandha breeding programs. Elsevier 2022-04 2022-01-20 /pmc/articles/PMC9073062/ /pubmed/35531148 http://dx.doi.org/10.1016/j.sjbs.2022.01.030 Text en © 2022 The Authors. Published by Elsevier B.V. on behalf of King Saud University. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Chauhan, Surya
Mandliya, Trapti
Jain, Devendra
Joshi, Arunabh
Khatik, Champa Lal
Singh, Abhijeet
Upadhyay, Sudhir K.
Jain, Rohit
Early selective strategies for higher yielding bio-economic Indian ginseng based on genotypic study through metabolic and molecular markers
title Early selective strategies for higher yielding bio-economic Indian ginseng based on genotypic study through metabolic and molecular markers
title_full Early selective strategies for higher yielding bio-economic Indian ginseng based on genotypic study through metabolic and molecular markers
title_fullStr Early selective strategies for higher yielding bio-economic Indian ginseng based on genotypic study through metabolic and molecular markers
title_full_unstemmed Early selective strategies for higher yielding bio-economic Indian ginseng based on genotypic study through metabolic and molecular markers
title_short Early selective strategies for higher yielding bio-economic Indian ginseng based on genotypic study through metabolic and molecular markers
title_sort early selective strategies for higher yielding bio-economic indian ginseng based on genotypic study through metabolic and molecular markers
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073062/
https://www.ncbi.nlm.nih.gov/pubmed/35531148
http://dx.doi.org/10.1016/j.sjbs.2022.01.030
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