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Theranostic system for ratiometric fluorescence monitoring of peptide-guided targeted drug delivery
Conjugation of an anticancer drug with a cancer-specific carrier and a fluorescent dye to form a theranostic system enables real time monitoring of targeted drug delivery (TDD). However, the fluorescence signal from the dye is affected by the light absorption and scattering in the body, photobleachi...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073098/ https://www.ncbi.nlm.nih.gov/pubmed/35529716 http://dx.doi.org/10.1039/c9ra06334j |
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author | Rozovsky, Alex Ebaston, T. M. Zaporozhets, Alisa Bazylevich, Andrii Tuchinsky, Helena Patsenker, Leonid Gellerman, Gary |
author_facet | Rozovsky, Alex Ebaston, T. M. Zaporozhets, Alisa Bazylevich, Andrii Tuchinsky, Helena Patsenker, Leonid Gellerman, Gary |
author_sort | Rozovsky, Alex |
collection | PubMed |
description | Conjugation of an anticancer drug with a cancer-specific carrier and a fluorescent dye to form a theranostic system enables real time monitoring of targeted drug delivery (TDD). However, the fluorescence signal from the dye is affected by the light absorption and scattering in the body, photobleaching, and instrumental parameters. Ratiometric measurements utilizing two fluorescence signals of different wavelengths are known to improve sensitivity, reliability and quantitation of fluorescence measurements in biological media. Herein, a novel theranostic system comprising the anticancer drug chlorambucil (CLB), cancer-specific peptide octreotide amide (OctA), and a long-wavelength dual fluorescent cyanine dye IRD enabling ratiometric monitoring of drug delivery was developed and evaluated on the cancer cell line PANC-1. |
format | Online Article Text |
id | pubmed-9073098 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90730982022-05-06 Theranostic system for ratiometric fluorescence monitoring of peptide-guided targeted drug delivery Rozovsky, Alex Ebaston, T. M. Zaporozhets, Alisa Bazylevich, Andrii Tuchinsky, Helena Patsenker, Leonid Gellerman, Gary RSC Adv Chemistry Conjugation of an anticancer drug with a cancer-specific carrier and a fluorescent dye to form a theranostic system enables real time monitoring of targeted drug delivery (TDD). However, the fluorescence signal from the dye is affected by the light absorption and scattering in the body, photobleaching, and instrumental parameters. Ratiometric measurements utilizing two fluorescence signals of different wavelengths are known to improve sensitivity, reliability and quantitation of fluorescence measurements in biological media. Herein, a novel theranostic system comprising the anticancer drug chlorambucil (CLB), cancer-specific peptide octreotide amide (OctA), and a long-wavelength dual fluorescent cyanine dye IRD enabling ratiometric monitoring of drug delivery was developed and evaluated on the cancer cell line PANC-1. The Royal Society of Chemistry 2019-10-14 /pmc/articles/PMC9073098/ /pubmed/35529716 http://dx.doi.org/10.1039/c9ra06334j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Rozovsky, Alex Ebaston, T. M. Zaporozhets, Alisa Bazylevich, Andrii Tuchinsky, Helena Patsenker, Leonid Gellerman, Gary Theranostic system for ratiometric fluorescence monitoring of peptide-guided targeted drug delivery |
title | Theranostic system for ratiometric fluorescence monitoring of peptide-guided targeted drug delivery |
title_full | Theranostic system for ratiometric fluorescence monitoring of peptide-guided targeted drug delivery |
title_fullStr | Theranostic system for ratiometric fluorescence monitoring of peptide-guided targeted drug delivery |
title_full_unstemmed | Theranostic system for ratiometric fluorescence monitoring of peptide-guided targeted drug delivery |
title_short | Theranostic system for ratiometric fluorescence monitoring of peptide-guided targeted drug delivery |
title_sort | theranostic system for ratiometric fluorescence monitoring of peptide-guided targeted drug delivery |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073098/ https://www.ncbi.nlm.nih.gov/pubmed/35529716 http://dx.doi.org/10.1039/c9ra06334j |
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