Cargando…

DEK modulates both expression and alternative splicing of cancer-related genes

DEK is known to be a potential proto-oncogene and is highly expressed in gastric cancer (GC); thus, DEK is considered to contribute to the malignant progression of GC. DEK is an RNA-binding protein involved in transcription, DNA repair, and selection of splicing sites during mRNA processing; however...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Bin, Sun, Yuanlin, Zhang, Yang, Xing, Yanpeng, Suo, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073418/
https://www.ncbi.nlm.nih.gov/pubmed/35475534
http://dx.doi.org/10.3892/or.2022.8322
_version_ 1784701281154105344
author Liu, Bin
Sun, Yuanlin
Zhang, Yang
Xing, Yanpeng
Suo, Jian
author_facet Liu, Bin
Sun, Yuanlin
Zhang, Yang
Xing, Yanpeng
Suo, Jian
author_sort Liu, Bin
collection PubMed
description DEK is known to be a potential proto-oncogene and is highly expressed in gastric cancer (GC); thus, DEK is considered to contribute to the malignant progression of GC. DEK is an RNA-binding protein involved in transcription, DNA repair, and selection of splicing sites during mRNA processing; however, its precise function remains elusive due to the lack of clarification of the overall profiles of gene transcription and post-transcriptional splicing that are regulated by DEK. We performed our original whole-genomic RNA-Seq data to analyze the global transcription and alternative splicing profiles in a human GC cell line by comparing DEK siRNA-treated and control conditions, dissecting both differential gene expression and potential alternative splicing events regulated by DEK. The siRNA-mediated knockdown of DEK in a GC cell line led to significant changes in gene expression of multiple cancer-related genes including both oncogenes and tumor suppressors. Moreover, it was revealed that DEK regulated a number of alternative splicing in genes which were significantly enriched in various cancer-related pathways including apoptosis and cell cycle processes. This study clarified for the first time that DEK has a regulatory effect on the alternative splicing, as well as on the expression, of numerous cancer-related genes, which is consistent with the role of DEK as a possible oncogene. Our results further expand the importance and feasibility of DEK as a clinical therapeutic target for human malignancies including GC.
format Online
Article
Text
id pubmed-9073418
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-90734182022-05-07 DEK modulates both expression and alternative splicing of cancer-related genes Liu, Bin Sun, Yuanlin Zhang, Yang Xing, Yanpeng Suo, Jian Oncol Rep Articles DEK is known to be a potential proto-oncogene and is highly expressed in gastric cancer (GC); thus, DEK is considered to contribute to the malignant progression of GC. DEK is an RNA-binding protein involved in transcription, DNA repair, and selection of splicing sites during mRNA processing; however, its precise function remains elusive due to the lack of clarification of the overall profiles of gene transcription and post-transcriptional splicing that are regulated by DEK. We performed our original whole-genomic RNA-Seq data to analyze the global transcription and alternative splicing profiles in a human GC cell line by comparing DEK siRNA-treated and control conditions, dissecting both differential gene expression and potential alternative splicing events regulated by DEK. The siRNA-mediated knockdown of DEK in a GC cell line led to significant changes in gene expression of multiple cancer-related genes including both oncogenes and tumor suppressors. Moreover, it was revealed that DEK regulated a number of alternative splicing in genes which were significantly enriched in various cancer-related pathways including apoptosis and cell cycle processes. This study clarified for the first time that DEK has a regulatory effect on the alternative splicing, as well as on the expression, of numerous cancer-related genes, which is consistent with the role of DEK as a possible oncogene. Our results further expand the importance and feasibility of DEK as a clinical therapeutic target for human malignancies including GC. D.A. Spandidos 2022-06 2022-04-21 /pmc/articles/PMC9073418/ /pubmed/35475534 http://dx.doi.org/10.3892/or.2022.8322 Text en Copyright: © Liu et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Liu, Bin
Sun, Yuanlin
Zhang, Yang
Xing, Yanpeng
Suo, Jian
DEK modulates both expression and alternative splicing of cancer-related genes
title DEK modulates both expression and alternative splicing of cancer-related genes
title_full DEK modulates both expression and alternative splicing of cancer-related genes
title_fullStr DEK modulates both expression and alternative splicing of cancer-related genes
title_full_unstemmed DEK modulates both expression and alternative splicing of cancer-related genes
title_short DEK modulates both expression and alternative splicing of cancer-related genes
title_sort dek modulates both expression and alternative splicing of cancer-related genes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073418/
https://www.ncbi.nlm.nih.gov/pubmed/35475534
http://dx.doi.org/10.3892/or.2022.8322
work_keys_str_mv AT liubin dekmodulatesbothexpressionandalternativesplicingofcancerrelatedgenes
AT sunyuanlin dekmodulatesbothexpressionandalternativesplicingofcancerrelatedgenes
AT zhangyang dekmodulatesbothexpressionandalternativesplicingofcancerrelatedgenes
AT xingyanpeng dekmodulatesbothexpressionandalternativesplicingofcancerrelatedgenes
AT suojian dekmodulatesbothexpressionandalternativesplicingofcancerrelatedgenes