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DEK modulates both expression and alternative splicing of cancer-related genes
DEK is known to be a potential proto-oncogene and is highly expressed in gastric cancer (GC); thus, DEK is considered to contribute to the malignant progression of GC. DEK is an RNA-binding protein involved in transcription, DNA repair, and selection of splicing sites during mRNA processing; however...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073418/ https://www.ncbi.nlm.nih.gov/pubmed/35475534 http://dx.doi.org/10.3892/or.2022.8322 |
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author | Liu, Bin Sun, Yuanlin Zhang, Yang Xing, Yanpeng Suo, Jian |
author_facet | Liu, Bin Sun, Yuanlin Zhang, Yang Xing, Yanpeng Suo, Jian |
author_sort | Liu, Bin |
collection | PubMed |
description | DEK is known to be a potential proto-oncogene and is highly expressed in gastric cancer (GC); thus, DEK is considered to contribute to the malignant progression of GC. DEK is an RNA-binding protein involved in transcription, DNA repair, and selection of splicing sites during mRNA processing; however, its precise function remains elusive due to the lack of clarification of the overall profiles of gene transcription and post-transcriptional splicing that are regulated by DEK. We performed our original whole-genomic RNA-Seq data to analyze the global transcription and alternative splicing profiles in a human GC cell line by comparing DEK siRNA-treated and control conditions, dissecting both differential gene expression and potential alternative splicing events regulated by DEK. The siRNA-mediated knockdown of DEK in a GC cell line led to significant changes in gene expression of multiple cancer-related genes including both oncogenes and tumor suppressors. Moreover, it was revealed that DEK regulated a number of alternative splicing in genes which were significantly enriched in various cancer-related pathways including apoptosis and cell cycle processes. This study clarified for the first time that DEK has a regulatory effect on the alternative splicing, as well as on the expression, of numerous cancer-related genes, which is consistent with the role of DEK as a possible oncogene. Our results further expand the importance and feasibility of DEK as a clinical therapeutic target for human malignancies including GC. |
format | Online Article Text |
id | pubmed-9073418 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-90734182022-05-07 DEK modulates both expression and alternative splicing of cancer-related genes Liu, Bin Sun, Yuanlin Zhang, Yang Xing, Yanpeng Suo, Jian Oncol Rep Articles DEK is known to be a potential proto-oncogene and is highly expressed in gastric cancer (GC); thus, DEK is considered to contribute to the malignant progression of GC. DEK is an RNA-binding protein involved in transcription, DNA repair, and selection of splicing sites during mRNA processing; however, its precise function remains elusive due to the lack of clarification of the overall profiles of gene transcription and post-transcriptional splicing that are regulated by DEK. We performed our original whole-genomic RNA-Seq data to analyze the global transcription and alternative splicing profiles in a human GC cell line by comparing DEK siRNA-treated and control conditions, dissecting both differential gene expression and potential alternative splicing events regulated by DEK. The siRNA-mediated knockdown of DEK in a GC cell line led to significant changes in gene expression of multiple cancer-related genes including both oncogenes and tumor suppressors. Moreover, it was revealed that DEK regulated a number of alternative splicing in genes which were significantly enriched in various cancer-related pathways including apoptosis and cell cycle processes. This study clarified for the first time that DEK has a regulatory effect on the alternative splicing, as well as on the expression, of numerous cancer-related genes, which is consistent with the role of DEK as a possible oncogene. Our results further expand the importance and feasibility of DEK as a clinical therapeutic target for human malignancies including GC. D.A. Spandidos 2022-06 2022-04-21 /pmc/articles/PMC9073418/ /pubmed/35475534 http://dx.doi.org/10.3892/or.2022.8322 Text en Copyright: © Liu et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Liu, Bin Sun, Yuanlin Zhang, Yang Xing, Yanpeng Suo, Jian DEK modulates both expression and alternative splicing of cancer-related genes |
title | DEK modulates both expression and alternative splicing of cancer-related genes |
title_full | DEK modulates both expression and alternative splicing of cancer-related genes |
title_fullStr | DEK modulates both expression and alternative splicing of cancer-related genes |
title_full_unstemmed | DEK modulates both expression and alternative splicing of cancer-related genes |
title_short | DEK modulates both expression and alternative splicing of cancer-related genes |
title_sort | dek modulates both expression and alternative splicing of cancer-related genes |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073418/ https://www.ncbi.nlm.nih.gov/pubmed/35475534 http://dx.doi.org/10.3892/or.2022.8322 |
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