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Molecular imprinting on PtPd nanoflowers for selective recognition and determination of hydrogen peroxide and glucose
PtPd nanoflowers (PtPd NFs) exhibit intrinsic peroxidase-like activity as nanozymes, but the nanozymes lack substrate specificity and have low catalytic activity. Herein, a molecularly imprinted nanogel on PtPd NFs was prepared by using 3,3′,5,5′-tetramethylbenzidine (TMB) as the template through th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073546/ https://www.ncbi.nlm.nih.gov/pubmed/35528877 http://dx.doi.org/10.1039/c9ra05677g |
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author | Fan, Caini Liu, Junjia Zhao, Haiying Li, Ling Liu, Min Gao, Jing Ma, Li |
author_facet | Fan, Caini Liu, Junjia Zhao, Haiying Li, Ling Liu, Min Gao, Jing Ma, Li |
author_sort | Fan, Caini |
collection | PubMed |
description | PtPd nanoflowers (PtPd NFs) exhibit intrinsic peroxidase-like activity as nanozymes, but the nanozymes lack substrate specificity and have low catalytic activity. Herein, a molecularly imprinted nanogel on PtPd NFs was prepared by using 3,3′,5,5′-tetramethylbenzidine (TMB) as the template through the aqueous precipitation polymerization method. After the TMB was washed out, many substrate binding pockets were retained in the PtPd NFs. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and powder X-ray diffraction (XRD) were employed to characterize the molecularly imprinted polymer (MIP) PtPd nanoflowers (T-MIP-PtPd NFs). The obtained T-MIP-PtPd NFs exhibited enhanced catalytic activity and specific recognition for TMB. Compared with PtPd NFs, T-MIP-PtPd NFs showed a linear range from 0.01–5000 μM and a detection limit of 0.005 μM toward the detection of H(2)O(2). Glucose can also be sensitively detected through cascade reaction by the T-MIP-PtPd NFs and glucose oxidase. Therefore, molecular imprinting on nanozymes technology shows promising application in biocatalysis and sensing fields. |
format | Online Article Text |
id | pubmed-9073546 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90735462022-05-06 Molecular imprinting on PtPd nanoflowers for selective recognition and determination of hydrogen peroxide and glucose Fan, Caini Liu, Junjia Zhao, Haiying Li, Ling Liu, Min Gao, Jing Ma, Li RSC Adv Chemistry PtPd nanoflowers (PtPd NFs) exhibit intrinsic peroxidase-like activity as nanozymes, but the nanozymes lack substrate specificity and have low catalytic activity. Herein, a molecularly imprinted nanogel on PtPd NFs was prepared by using 3,3′,5,5′-tetramethylbenzidine (TMB) as the template through the aqueous precipitation polymerization method. After the TMB was washed out, many substrate binding pockets were retained in the PtPd NFs. Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and powder X-ray diffraction (XRD) were employed to characterize the molecularly imprinted polymer (MIP) PtPd nanoflowers (T-MIP-PtPd NFs). The obtained T-MIP-PtPd NFs exhibited enhanced catalytic activity and specific recognition for TMB. Compared with PtPd NFs, T-MIP-PtPd NFs showed a linear range from 0.01–5000 μM and a detection limit of 0.005 μM toward the detection of H(2)O(2). Glucose can also be sensitively detected through cascade reaction by the T-MIP-PtPd NFs and glucose oxidase. Therefore, molecular imprinting on nanozymes technology shows promising application in biocatalysis and sensing fields. The Royal Society of Chemistry 2019-10-21 /pmc/articles/PMC9073546/ /pubmed/35528877 http://dx.doi.org/10.1039/c9ra05677g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Fan, Caini Liu, Junjia Zhao, Haiying Li, Ling Liu, Min Gao, Jing Ma, Li Molecular imprinting on PtPd nanoflowers for selective recognition and determination of hydrogen peroxide and glucose |
title | Molecular imprinting on PtPd nanoflowers for selective recognition and determination of hydrogen peroxide and glucose |
title_full | Molecular imprinting on PtPd nanoflowers for selective recognition and determination of hydrogen peroxide and glucose |
title_fullStr | Molecular imprinting on PtPd nanoflowers for selective recognition and determination of hydrogen peroxide and glucose |
title_full_unstemmed | Molecular imprinting on PtPd nanoflowers for selective recognition and determination of hydrogen peroxide and glucose |
title_short | Molecular imprinting on PtPd nanoflowers for selective recognition and determination of hydrogen peroxide and glucose |
title_sort | molecular imprinting on ptpd nanoflowers for selective recognition and determination of hydrogen peroxide and glucose |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073546/ https://www.ncbi.nlm.nih.gov/pubmed/35528877 http://dx.doi.org/10.1039/c9ra05677g |
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