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Identification of novel proteins in the Dictyostelium discoideum chemorepulsion pathway using REMI
Chemorepulsion, the biased migration of a cell away from a signal, is essential for many biological processes and the ability to manipulate chemorepulsion could lead to new therapeutics for a variety of diseases. However, little is known about eukaryotic cell chemorepulsion. Utilizing the model orga...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073555/ https://www.ncbi.nlm.nih.gov/pubmed/35622529 http://dx.doi.org/10.17912/micropub.biology.000557 |
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author | Kirolos, Sara A Procaccia, Shiri Groover, Kyra E Das, Rheeturaag Rijal, Ramesh Gomer, Richard H |
author_facet | Kirolos, Sara A Procaccia, Shiri Groover, Kyra E Das, Rheeturaag Rijal, Ramesh Gomer, Richard H |
author_sort | Kirolos, Sara A |
collection | PubMed |
description | Chemorepulsion, the biased migration of a cell away from a signal, is essential for many biological processes and the ability to manipulate chemorepulsion could lead to new therapeutics for a variety of diseases. However, little is known about eukaryotic cell chemorepulsion. Utilizing the model organism Dictyostelium discoideum, we previously identified an endogenous chemorepellent protein secreted by D. discoideum cells called AprA, and proteins involved in the AprA-induced chemorepulsion pathway including the G protein-coupled receptor GrlH, G beta and G protein alpha 8 protein subunits, protein kinase A, components of the mammalian target of rapamycin complex 2 (mTORC2), phospholipase A, PTEN and a PTEN-like phosphatase (CnrN), a retinoblastoma orthologue (RblA), extracellular signal-regulated kinase 1 (Erk1), p-21 activated protein kinase D (PakD), and the Ras proteins RasC and RasG. In this report, we used a genetic screen to identify 17 additional proteins involved in the AprA-induced chemorepulsion pathway . |
format | Online Article Text |
id | pubmed-9073555 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-90735552022-05-07 Identification of novel proteins in the Dictyostelium discoideum chemorepulsion pathway using REMI Kirolos, Sara A Procaccia, Shiri Groover, Kyra E Das, Rheeturaag Rijal, Ramesh Gomer, Richard H MicroPubl Biol New Finding Chemorepulsion, the biased migration of a cell away from a signal, is essential for many biological processes and the ability to manipulate chemorepulsion could lead to new therapeutics for a variety of diseases. However, little is known about eukaryotic cell chemorepulsion. Utilizing the model organism Dictyostelium discoideum, we previously identified an endogenous chemorepellent protein secreted by D. discoideum cells called AprA, and proteins involved in the AprA-induced chemorepulsion pathway including the G protein-coupled receptor GrlH, G beta and G protein alpha 8 protein subunits, protein kinase A, components of the mammalian target of rapamycin complex 2 (mTORC2), phospholipase A, PTEN and a PTEN-like phosphatase (CnrN), a retinoblastoma orthologue (RblA), extracellular signal-regulated kinase 1 (Erk1), p-21 activated protein kinase D (PakD), and the Ras proteins RasC and RasG. In this report, we used a genetic screen to identify 17 additional proteins involved in the AprA-induced chemorepulsion pathway . Caltech Library 2022-05-05 /pmc/articles/PMC9073555/ /pubmed/35622529 http://dx.doi.org/10.17912/micropub.biology.000557 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Finding Kirolos, Sara A Procaccia, Shiri Groover, Kyra E Das, Rheeturaag Rijal, Ramesh Gomer, Richard H Identification of novel proteins in the Dictyostelium discoideum chemorepulsion pathway using REMI |
title |
Identification of novel proteins in the
Dictyostelium discoideum
chemorepulsion pathway using REMI
|
title_full |
Identification of novel proteins in the
Dictyostelium discoideum
chemorepulsion pathway using REMI
|
title_fullStr |
Identification of novel proteins in the
Dictyostelium discoideum
chemorepulsion pathway using REMI
|
title_full_unstemmed |
Identification of novel proteins in the
Dictyostelium discoideum
chemorepulsion pathway using REMI
|
title_short |
Identification of novel proteins in the
Dictyostelium discoideum
chemorepulsion pathway using REMI
|
title_sort | identification of novel proteins in the
dictyostelium discoideum
chemorepulsion pathway using remi |
topic | New Finding |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073555/ https://www.ncbi.nlm.nih.gov/pubmed/35622529 http://dx.doi.org/10.17912/micropub.biology.000557 |
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