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Whole-genome sequencing identifies I-SceI-mediated transgene integration sites in Xenopus tropicalis snai2:eGFP line

Transgenesis with the meganuclease I-SceI is a safe and efficient method, but the underlying mechanisms remain unclear due to the lack of information on transgene localization. Using I-SceI, we previously developed a transgenic Xenopus tropicalis line expressing enhanced green fluorescent protein dr...

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Detalles Bibliográficos
Autores principales: Wang, Jian, Lu, Congyu, Wei, Shuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073676/
https://www.ncbi.nlm.nih.gov/pubmed/35171990
http://dx.doi.org/10.1093/g3journal/jkac037