Cargando…
Whole-genome sequencing identifies I-SceI-mediated transgene integration sites in Xenopus tropicalis snai2:eGFP line
Transgenesis with the meganuclease I-SceI is a safe and efficient method, but the underlying mechanisms remain unclear due to the lack of information on transgene localization. Using I-SceI, we previously developed a transgenic Xenopus tropicalis line expressing enhanced green fluorescent protein dr...
Autores principales: | Wang, Jian, Lu, Congyu, Wei, Shuo |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073676/ https://www.ncbi.nlm.nih.gov/pubmed/35171990 http://dx.doi.org/10.1093/g3journal/jkac037 |
Ejemplares similares
-
Structural stability and endonuclease activity of a PI-SceI GFP-fusion protein
por: Senejani, Alireza G., et al.
Publicado: (2007) -
The Meganuclease I-SceI Containing Nuclear Localization Signal (NLS-I-SceI) Efficiently Mediated Mammalian Germline Transgenesis via Embryo Cytoplasmic Microinjection
por: Wang, Yong, et al.
Publicado: (2014) -
Novel reporter systems for facile evaluation of I-SceI-mediated genome editing
por: Muñoz, Nina M., et al.
Publicado: (2012) -
Use of the Meganuclease I-SceI of Saccharomyces
cerevisiae to select for gene deletions in actinomycetes
por: Fernández-Martínez, Lorena T., et al.
Publicado: (2014) -
Utility of I-SceI and CCR5-ZFN nucleases in excising selectable marker genes from transgenic plants
por: Pathak, Bhuvan P., et al.
Publicado: (2019)