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Enemy or ally: a genomic approach to elucidate the lifestyle of Phyllosticta citrichinaensis
Members of the fungal genus Phyllosticta can colonize a variety of plant hosts, including several Citrus species such as Citrus sinensis (orange), Citrus limon (lemon), and Citrus maxima (pomelo). Some Phyllosticta species have the capacity to cause disease, such as Citrus Black Spot, while others h...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073689/ https://www.ncbi.nlm.nih.gov/pubmed/35311955 http://dx.doi.org/10.1093/g3journal/jkac061 |
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author | Buijs, Valerie A Groenewald, Johannes Z Haridas, Sajeet LaButti, Kurt M Lipzen, Anna Martin, Francis M Barry, Kerrie Grigoriev, Igor V Crous, Pedro W Seidl, Michael F |
author_facet | Buijs, Valerie A Groenewald, Johannes Z Haridas, Sajeet LaButti, Kurt M Lipzen, Anna Martin, Francis M Barry, Kerrie Grigoriev, Igor V Crous, Pedro W Seidl, Michael F |
author_sort | Buijs, Valerie A |
collection | PubMed |
description | Members of the fungal genus Phyllosticta can colonize a variety of plant hosts, including several Citrus species such as Citrus sinensis (orange), Citrus limon (lemon), and Citrus maxima (pomelo). Some Phyllosticta species have the capacity to cause disease, such as Citrus Black Spot, while others have only been observed as endophytes. Thus far, genomic differences underlying lifestyle adaptations of Phyllosticta species have not yet been studied. Furthermore, the lifestyle of Phyllosticta citrichinaensis is ambiguous, as it has been described as a weak pathogen but Koch’s postulates may not have been established and the presence of this species was never reported to cause any crop or economic losses. Here, we examined the genomic differences between pathogenic and endophytic Phyllosticta spp. colonizing Citrus and specifically aimed to elucidate the lifestyle of Phyllosticta citrichinaensis. We found several genomic differences between species of different lifestyles, including groups of genes that were only present in pathogens or endophytes. We also observed that species, based on their carbohydrate active enzymes, group independent of their phylogenetic association, and this clustering correlated with trophy prediction. Phyllosticta citrichinaensis shows an intermediate lifestyle, sharing genomic and phenotypic attributes of both pathogens and endophytes. We thus present the first genomic comparison of multiple citrus-colonizing pathogens and endophytes of the genus Phyllosticta, and therefore provide the basis for further comparative studies into the lifestyle adaptations within this genus. |
format | Online Article Text |
id | pubmed-9073689 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-90736892022-05-06 Enemy or ally: a genomic approach to elucidate the lifestyle of Phyllosticta citrichinaensis Buijs, Valerie A Groenewald, Johannes Z Haridas, Sajeet LaButti, Kurt M Lipzen, Anna Martin, Francis M Barry, Kerrie Grigoriev, Igor V Crous, Pedro W Seidl, Michael F G3 (Bethesda) Investigation Members of the fungal genus Phyllosticta can colonize a variety of plant hosts, including several Citrus species such as Citrus sinensis (orange), Citrus limon (lemon), and Citrus maxima (pomelo). Some Phyllosticta species have the capacity to cause disease, such as Citrus Black Spot, while others have only been observed as endophytes. Thus far, genomic differences underlying lifestyle adaptations of Phyllosticta species have not yet been studied. Furthermore, the lifestyle of Phyllosticta citrichinaensis is ambiguous, as it has been described as a weak pathogen but Koch’s postulates may not have been established and the presence of this species was never reported to cause any crop or economic losses. Here, we examined the genomic differences between pathogenic and endophytic Phyllosticta spp. colonizing Citrus and specifically aimed to elucidate the lifestyle of Phyllosticta citrichinaensis. We found several genomic differences between species of different lifestyles, including groups of genes that were only present in pathogens or endophytes. We also observed that species, based on their carbohydrate active enzymes, group independent of their phylogenetic association, and this clustering correlated with trophy prediction. Phyllosticta citrichinaensis shows an intermediate lifestyle, sharing genomic and phenotypic attributes of both pathogens and endophytes. We thus present the first genomic comparison of multiple citrus-colonizing pathogens and endophytes of the genus Phyllosticta, and therefore provide the basis for further comparative studies into the lifestyle adaptations within this genus. Oxford University Press 2022-03-21 /pmc/articles/PMC9073689/ /pubmed/35311955 http://dx.doi.org/10.1093/g3journal/jkac061 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Genetics Society of America. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Investigation Buijs, Valerie A Groenewald, Johannes Z Haridas, Sajeet LaButti, Kurt M Lipzen, Anna Martin, Francis M Barry, Kerrie Grigoriev, Igor V Crous, Pedro W Seidl, Michael F Enemy or ally: a genomic approach to elucidate the lifestyle of Phyllosticta citrichinaensis |
title | Enemy or ally: a genomic approach to elucidate the lifestyle of Phyllosticta citrichinaensis |
title_full | Enemy or ally: a genomic approach to elucidate the lifestyle of Phyllosticta citrichinaensis |
title_fullStr | Enemy or ally: a genomic approach to elucidate the lifestyle of Phyllosticta citrichinaensis |
title_full_unstemmed | Enemy or ally: a genomic approach to elucidate the lifestyle of Phyllosticta citrichinaensis |
title_short | Enemy or ally: a genomic approach to elucidate the lifestyle of Phyllosticta citrichinaensis |
title_sort | enemy or ally: a genomic approach to elucidate the lifestyle of phyllosticta citrichinaensis |
topic | Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9073689/ https://www.ncbi.nlm.nih.gov/pubmed/35311955 http://dx.doi.org/10.1093/g3journal/jkac061 |
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