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Probing relaxation dynamics of a cationic lipid based non-viral carrier: a time-resolved fluorescence study

Lipid vesicles composed of cationic dioctadecyldimethylammonium bromide (DODAB) and neutral 1-monooleoyl-rac-glycerol (MO) are promising non-viral carriers of nucleic acids for delivery into cells. Among them, higher cell transfection efficiency was displayed by DODAB-rich vesicles than those enrich...

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Autores principales: Singh, Priya, Mukherjee, Dipanjan, Singha, Subhankar, Sharma, V. K., Althagafi, Ismail I., Ahmed, Saleh A., Mukhopadhyay, R., Das, Ranjan, Pal, Samir Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074709/
https://www.ncbi.nlm.nih.gov/pubmed/35528090
http://dx.doi.org/10.1039/c9ra06824d
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author Singh, Priya
Mukherjee, Dipanjan
Singha, Subhankar
Sharma, V. K.
Althagafi, Ismail I.
Ahmed, Saleh A.
Mukhopadhyay, R.
Das, Ranjan
Pal, Samir Kumar
author_facet Singh, Priya
Mukherjee, Dipanjan
Singha, Subhankar
Sharma, V. K.
Althagafi, Ismail I.
Ahmed, Saleh A.
Mukhopadhyay, R.
Das, Ranjan
Pal, Samir Kumar
author_sort Singh, Priya
collection PubMed
description Lipid vesicles composed of cationic dioctadecyldimethylammonium bromide (DODAB) and neutral 1-monooleoyl-rac-glycerol (MO) are promising non-viral carriers of nucleic acids for delivery into cells. Among them, higher cell transfection efficiency was displayed by DODAB-rich vesicles than those enriched with MO. Structural relaxation of these mixed lipid vesicles plays a key role in their cell transfection efficiency because structural organization of the DODAB-rich vesicles are different from that of the MO-rich vesicles. Polarization-gated anisotropy in conjunction with picosecond resolved emission transients of a novel fluorophore 6-acetyl-(2-((4-hydroxycyclohexyl)(methyl)amino)naphthalene) (ACYMAN) has been employed to probe relaxation dynamics in pure DODAB vesicles, and in mixed vesicles of DODAB with varying content of MO. Both orientational relaxation of ACYMAN and relaxation dynamics of its local environment are retarded significantly in mixed lipid vesicles with increasing MO content, from a mole fraction (χ(MO)) of 0.2 to that of 0.8 which is consistent with increased rigidity of the MO-rich (χ(MO) > 0.5) vesicles relative to the DODAB-rich (χ(MO) < 0.5) vesicles. Therefore, higher structural rigidity of the MO-rich vesicles (χ(MO) > 0.5) gives rise to their lower cell transfection efficiency than the more flexible DODAB-rich (χ(MO) < 0.5) vesicles as observed in previous in vivo studies (Biochim. Biophys. Acta, Biomembr., 2014, 1838, 2555–2567).
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spelling pubmed-90747092022-05-06 Probing relaxation dynamics of a cationic lipid based non-viral carrier: a time-resolved fluorescence study Singh, Priya Mukherjee, Dipanjan Singha, Subhankar Sharma, V. K. Althagafi, Ismail I. Ahmed, Saleh A. Mukhopadhyay, R. Das, Ranjan Pal, Samir Kumar RSC Adv Chemistry Lipid vesicles composed of cationic dioctadecyldimethylammonium bromide (DODAB) and neutral 1-monooleoyl-rac-glycerol (MO) are promising non-viral carriers of nucleic acids for delivery into cells. Among them, higher cell transfection efficiency was displayed by DODAB-rich vesicles than those enriched with MO. Structural relaxation of these mixed lipid vesicles plays a key role in their cell transfection efficiency because structural organization of the DODAB-rich vesicles are different from that of the MO-rich vesicles. Polarization-gated anisotropy in conjunction with picosecond resolved emission transients of a novel fluorophore 6-acetyl-(2-((4-hydroxycyclohexyl)(methyl)amino)naphthalene) (ACYMAN) has been employed to probe relaxation dynamics in pure DODAB vesicles, and in mixed vesicles of DODAB with varying content of MO. Both orientational relaxation of ACYMAN and relaxation dynamics of its local environment are retarded significantly in mixed lipid vesicles with increasing MO content, from a mole fraction (χ(MO)) of 0.2 to that of 0.8 which is consistent with increased rigidity of the MO-rich (χ(MO) > 0.5) vesicles relative to the DODAB-rich (χ(MO) < 0.5) vesicles. Therefore, higher structural rigidity of the MO-rich vesicles (χ(MO) > 0.5) gives rise to their lower cell transfection efficiency than the more flexible DODAB-rich (χ(MO) < 0.5) vesicles as observed in previous in vivo studies (Biochim. Biophys. Acta, Biomembr., 2014, 1838, 2555–2567). The Royal Society of Chemistry 2019-11-01 /pmc/articles/PMC9074709/ /pubmed/35528090 http://dx.doi.org/10.1039/c9ra06824d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Singh, Priya
Mukherjee, Dipanjan
Singha, Subhankar
Sharma, V. K.
Althagafi, Ismail I.
Ahmed, Saleh A.
Mukhopadhyay, R.
Das, Ranjan
Pal, Samir Kumar
Probing relaxation dynamics of a cationic lipid based non-viral carrier: a time-resolved fluorescence study
title Probing relaxation dynamics of a cationic lipid based non-viral carrier: a time-resolved fluorescence study
title_full Probing relaxation dynamics of a cationic lipid based non-viral carrier: a time-resolved fluorescence study
title_fullStr Probing relaxation dynamics of a cationic lipid based non-viral carrier: a time-resolved fluorescence study
title_full_unstemmed Probing relaxation dynamics of a cationic lipid based non-viral carrier: a time-resolved fluorescence study
title_short Probing relaxation dynamics of a cationic lipid based non-viral carrier: a time-resolved fluorescence study
title_sort probing relaxation dynamics of a cationic lipid based non-viral carrier: a time-resolved fluorescence study
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074709/
https://www.ncbi.nlm.nih.gov/pubmed/35528090
http://dx.doi.org/10.1039/c9ra06824d
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